Method for promoting cellulolytic enzymes synthesis of trichoderma reesei by using dilute sulfuric acid treated pulp as carbon source

Abstract

The invention relates to a method for taking paper pulp as raw materials and as a carbon source which is needed for synthesizing cellulose enzyme by trichoderma reesei after the paper pulp is processed with dilute sulfuric acid. Compared with a method for taking pure cellulose as the carbon source, the filter paper enzyme activity can be increased by 40% through using the method for preparing thecellulose enzyme, and the problems that somatic cell is lack of the ability to utilize cellulose and the activity of the cellulose enzyme is not high when the paper pulp and dextrose are taken as thecarbon source to prepare culture medium are solved.

Description

Dilute sulfuric acid treatment of pulp as a carbon source to promote the synthesis of cellulase by Trichoderma reesei 1. Technical field The invention belongs to the technical field of microbial cultivation in biochemistry, and particularly relates to a method for treating pulp with dilute sulfuric acid as a carbon source to promote the synthesis of cellulase by Trichoderma reesei. 2. Background technology Prior art: cellulase is a general term for a group of enzymes used to degrade cellulose, the main components are endoglucanase (endoglucanase), exoglucanase (exoglucanase) and β-glucose Glucosidase (β-glucosidase). The mechanism of cellulase degrading cellulose is: endoglucanase randomly cuts cellulose macromolecules into short-chain cellulose, and exoglucanase cuts short-chain cellulose ends to form cellobiose or oligosaccharides, β - Glucosidase acts on cellobiose and oligosaccharides to form glucose. The strains that can be used to produce cellulase mainly include T...

AI Technical Summary

Problems solved by technology

General lignocellulosic raw materials (such as pulp) are difficult to meet the requirements of providing both components

Monosaccharides such as glucose are often added in production to promote the growth of bacterial cells, but bacterial cells grown with glucose as a carbon source lack the ability to utilize cellulose, resulting in low cellulase activity

So far, no lower cost, equivalent or better alternative carbon source to promote the synthesis of cellulase by Trichoderma reesei has been found

Images