Composition with sleep aiding and skin repairing effects as well as preparation method and application thereof

Abstract

The invention relates to a composition with sleep aiding and skin repairing effects and a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a composition with sleep aiding and skin repairing effects. The composition is prepared from the following components in parts by weight: 72 to 114 parts of compound plant essential oil, 3 to 5 parts of ceramide NP and 1 to 3 parts of sodium hyaluronate, the compound plant essential oil is prepared from lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil and rhodiola rosea essential oil; the rhodiola rosea essential oil is obtained by fermenting a mixed strain composed of kluyveromyces lactis and trichoderma asperellum and then extracting essential oil. The research shows that the mixed strain fermentation can synergistically improve the extraction rate of the rhodiola rosea essential oil. In addition, the compound plant essential oil provided by the invention can also synergistically improve the anti-inflammatory, moisturizing and anti-allergy repair capabilities of the composition and has the effect of improving sleep disorders, so that the composition has better sleep aiding and skin repairing effects at the same time.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a composition with sleep-aiding and skin repairing effects, and a preparation method and application thereof. BACKGROUND

[0002] Due to the increasing pressure from various aspects, more and more modern people are troubled by sleep disorders. Long-term sleep disorders can easily lead to problems such as reduced immune function, fatigue, and memory loss. At the same time, sleep disorders and skin sub-health status are also significantly related. Declining sleep quality can lead to weakened skin barrier function and exacerbated inflammatory reactions, and skin discomfort can further affect sleep stability.

[0003] Currently, the use of drugs to treat sleep disorders often has various side effects. Therefore, the conditioning method of sleep-aiding essential oil health products, i.e., essential oil therapy, has attracted much attention. Compared with drug therapy, this therapy can effectively avoid the drawbacks of addiction and dependence. Essential oil therapy is considered an effective intervention in improving sleep problems, and plant essential oils commonly used for sleep-aiding include lavender essential oil, sweet orange essential oil, and chamomile essential oil. In addition, plant essential oils have obvious advantages in improving skin conditions due to their natural and mild properties. In the prior art, sleep-aiding cosmetics mostly rely on a single essential oil component, and the addition amount is too high (often more than 5%), which can easily cause skin irritation. The additives of skin repairing cosmetics lack systematic regulation of the nerve-skin axis, and cannot meet the dual needs of sleep-aiding and skin repairing. Therefore, there is still a lack of a product with low addition amount and dual effects of sleep-aiding and skin repairing.

[0004] Rhodiola rosea L. is a plant of the genus Rhodiola in the family Crassulaceae, which has various pharmacological activities. Its extract has been widely used in the field of cosmetics, and has skin care effects such as antioxidant, moisturizing, and repairing. However, the application of Rhodiola rosea essential oil is rarely seen in the prior art, and the extraction rate of Rhodiola rosea essential oil is low, which is one of the reasons limiting its application. Therefore, it is of great significance to provide a method for improving the extraction yield of Rhodiola rosea essential oil. SUMMARY

[0005] The present application relates to the field of cosmetics, in particular to a composition with sleep-aiding and skin repairing effects, and a preparation method and application thereof.

[0006] To achieve the above-mentioned purposes, the technical solutions adopted by the present application are as follows: In a first aspect, the present application provides a composition with sleep-aiding and skin repairing effects, comprising the following components in parts by weight: compound plant essential oil 72-114 parts, ceramide NP 3-5 parts, and sodium hyaluronate 1-3 parts; the compound plant essential oil is composed of lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil, and rhodiola essential oil in a mass ratio of (20-35):(16-25):(20-30):(6-9):(10-15); the rhodiola essential oil is obtained by inoculating mixed strains on rhodiola as raw material for fermentation and extracting essential oil from the rhodiola fermentation product; the mixed strains are Kluyveromyces lactis ( Kluyveromyces lactis ) and Trichoderma asperellum ( Trichoderma asperellum ).

[0007] Firstly, the present application finds that the mixed fermentation of specific Kluyveromyces lactis and Trichoderma asperellum helps to improve the extraction rate of rhodiola essential oil, which is increased by 59.30%-68.60% compared with the unfermented rhodiola essential oil. Secondly, the present application compounds the rhodiola essential oil extracted after fermentation with lavender essential oil, sweet orange essential oil, chamomile essential oil, and rosemary essential oil to obtain a compound plant essential oil, and then combines it with ceramide NP and sodium hyaluronate as an efficacy composition. The anti-inflammatory, skin repairing, and sleep-aiding effects of the efficacy composition are determined and compared with those of efficacy compositions with different component compositions. The experimental results show that the compound plant essential oil, ceramide NP, and sodium hyaluronate in the composition provided by the present application can significantly improve the anti-inflammatory effect, skin moisturizing ability, and anti-allergic ability of the composition. In addition, the composition provided by the present application also has a significant ability to improve sleep disorders, and the plant essential oil components in the compound plant essential oil produce a synergistic effect in improving the sleep of subjects with sleep disorders. Therefore, the composition provided by the present application has good sleep-aiding and skin repairing effects.

[0008] Further, the composition comprises the following components in parts by weight: compound plant essential oil 93-114 parts, ceramide NP 4-5 parts, and sodium hyaluronate 2-3 parts.

[0009] Preferably, the composition comprises the following components in parts by weight: compound plant essential oil 100 parts, ceramide NP 4 parts, and sodium hyaluronate 2 parts.

[0010] Further, the compound plant essential oil is composed of lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil, and rhodiola essential oil in a mass ratio of (28-35):(20-25):(25-30):(7.5-9):(12.5-15).

[0011] Preferably, the compound plant essential oil is composed of lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil, and rhodiola essential oil in a mass ratio of 30:22:28:8:12.

[0012] Further, the fermentation conditions are that 1-2% of a Kluyveromyces lactis liquid with a viable cell count of 10 7 -10 9 CFU / mL and 3-5% of a Trichoderma spinosum liquid with a spore count of 10 6 -10 7 CFU / mL are inoculated and fermented at 25-30℃ for 3-5 days.

[0013] Preferably, the fermentation conditions are that 2% of a Kluyveromyces lactis liquid with a viable cell count of 10 8 CFU / mL and 4% of a Trichoderma spinosum liquid with a spore count of 10 7 CFU / mL are inoculated and fermented at 28℃ for 4 days.

[0014] Further, the red sage oil is extracted by a supercritical fluid extraction method.

[0015] Further, the lavender oil, chamomile oil and rosemary oil are respectively extracted by a steam distillation extraction method using lavender, chamomile and rosemary as raw materials.

[0016] Preferably, the chamomile is Roman chamomile.

[0017] Preferably, the Kluyveromyces lactis is Kluyveromyces lactis JNXR-2101, which is preserved in the China Center for Type Culture Collection (CCTCC) with a preservation number of CCTCC NO: M 20211628.

[0018] Preferably, the Trichoderma spinosum is Trichoderma spinosum SFC-3, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC No.16097.

[0019] In a second aspect, the present application provides use of any of the above-mentioned compositions with sleep-aiding and skin-protection effects in the preparation of sleep-aiding and skin-protection cosmetics.

[0020] Further, the mass percentage of the composition with sleep-aiding and skin-protection effects is 0.1-3%.

[0021] Further, the dosage form of the cosmetic includes any one of cream, water agent, emulsion and oil agent.

[0022] In a third aspect, the present application provides a sleep-aiding and skin-protection cosmetic containing 0.1-3% of any of the above-mentioned compositions with sleep-aiding and skin-protection effects.

[0023] Further, it also contains adjuvants acceptable in the field of cosmetics.

[0024] Preferably, the adjuvant includes any one or several of emulsifiers, humectants, emollients, pH adjusters, penetration enhancers, preservatives, deionized water.

[0025] Compared with the prior art, the present application has the following beneficial effects: The present application provides a composition with sleep-aiding and skin repairing effects, comprising the following components in parts by weight: compound plant essential oil 72-114 parts, ceramide NP 3-5 parts, and sodium hyaluronate 1-3 parts; the compound plant essential oil is composed of lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil and rhodiola essential oil in a mass ratio of (20-35):(16-25):(20-30):(6-9):(10-15); the rhodiola essential oil is obtained by fermenting rhodiola with a mixed strain consisting of Kluyveromyces lactis and Trichoderma asperellum and extracting essential oil from the rhodiola fermentation product. The research of the present application shows that the mixed strain fermentation can synergistically improve the extraction rate of rhodiola essential oil. In addition, the compound plant essential oil provided by the present application can also synergistically improve the anti-inflammatory, moisturizing and anti-allergic repair abilities of the composition and has the effect of improving sleep disorders, so that the composition has good sleep-aiding and skin repairing effects. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 The extraction rate of rhodiola essential oil in Examples 1-2 and Comparative Examples 1-5. DETAILED DESCRIPTION

[0027] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0028] The Kluyveromyces lactis used in Examples 1-2, Comparative Example 2 and Comparative Example 5 is Kluyveromyces lactis Kluyveromyces lactis JNXR-2101, preserved in China Center for Type Culture Collection, with the preservation number CCTCC NO: M 20211628.

[0029] The Trichoderma asperellum used in Examples 1-2 and Comparative Examples 3-4 is Trichoderma asperellum Trichoderma asperellum SFC-3, preserved in China General Microbiological Culture Collection Center, with the preservation number CGMCC No. 16097.

[0030] Ceramide NP, purchased from Hangzhou Weipulai Biological Technology Co., Ltd., product number: VBL-NP-077; Sodium hyaluronate, purchased from Yixing Biological Technology Co., Ltd., product number: 61006ES03.

[0031] Other materials, reagents used in the examples are commercially available unless otherwise stated.

[0032] Preparation of raw materials: Preparation of lavender essential oil: Take 200g of lavender, wash and dry it, then crush it, and soak it in water at 40°C for 3 hours. After soaking, add 15 times the weight of the lavender of deionized water to it for distillation, and use 8.5% of NaCl solid equivalent to the weight of the lavender used as an aid, control the distillation temperature at 110°C, and the distillation time for 5 hours. Collect the distillate after condensation. Add petroleum ether (0.5 times the volume of the distillate) to the collected distillate, and place the mixture in an ultrasonic shaker at 60°C for 10 minutes. After the shaking is completed, let the mixture stand and separate, collect the upper layer solution, and repeat the ultrasonic shaking operation for the lower layer solution twice. Finally, combine the upper layer solutions obtained in the three operations, and perform rotary evaporation concentration at 45°C until no liquid is evaporated, and lavender essential oil is obtained.

[0033] Preparation of chamomile essential oil: Take 200g of Roman chamomile, wash and dry it, then crush it, and soak it in water at 40°C for 3 hours. After soaking, add 15 times the weight of the chamomile of deionized water to it for distillation, and use 8.5% of NaCl solid equivalent to the weight of the chamomile used as an aid, control the distillation temperature at 110°C, and the distillation time for 5 hours. Collect the distillate after condensation. Add petroleum ether (0.5 times the volume of the distillate) to the collected distillate, and place the mixture in an ultrasonic shaker at 60°C for 10 minutes. After the shaking is completed, let the mixture stand and separate, collect the upper layer solution, and repeat the ultrasonic shaking operation for the lower layer solution twice. Finally, combine the upper layer solutions obtained in the three operations, and perform rotary evaporation concentration at 45°C until no liquid is evaporated, and chamomile essential oil is obtained.

[0034] Rosemary essential oil preparation method: 200 g of rosemary was washed, dried and crushed, and then sieved through a 100 mesh sieve. The sieved rosemary was soaked in water at 40°C for 3 hours. After soaking, 15 times the weight of the rosemary in deionized water was added to the rosemary for distillation. NaCl solid was used as an aid, which was 8.5% of the weight of the rosemary. The distillation temperature was controlled at 110°C, and the distillation time was 5 hours. After condensation, the distillate was collected. Petroleum ether was added to the collected distillate (the volume of petroleum ether was 0.5 times the volume of the distillate), and the mixture was placed in an ultrasonic shaker and shaken at 60°C for 10 minutes. After shaking, the mixture was allowed to stand and separate into layers. The upper layer was collected, and the lower layer was subjected to the above ultrasonic shaking operation twice. Finally, the upper layers obtained in the three operations were combined and concentrated by rotary evaporation at 45°C until no liquid was evaporated. Rosemary essential oil was obtained.

[0035] Sweet orange essential oil preparation method: fresh orange peel was soaked in 0.5 g / 100 mL calcium chloride solution for 2 h, wherein the ratio of orange peel to calcium chloride solution was 1 g:10 mL. The soaked orange peel was washed with clean water, placed in a three-type press, and then the obtained mixture was allowed to stand and separate into layers for 20 min. The upper layer mixture was centrifuged at 6 000 r / min for 20 min, and the upper layer essential oil was collected and dried with anhydrous sodium sulfate. The obtained essential oil was stored in a 4°C refrigerator in the dark.

[0036] Example 1 Preparation of Rhodiolae Crenulatae Essential Oil 500 g of washed and dried Rhodiolae Crenulatae roots and stems were crushed and sieved through an 80 mesh sieve to obtain Rhodiolae Crenulatae powder. The Rhodiolae Crenulatae powder was mixed with deionized water at a mass ratio of 1:1, sterilized at 121°C for 20 min, and then removed after cooling to obtain a solid-state fermentation material. A Kluyveromyces lactis liquid with a viable bacterial count of 1×10 8 CFU / mL and a Trichoderma spinosum liquid with a spore count of 1×10 7 CFU / mL were mixed at 2wt% and 4wt% of the mass of the solid-state fermentation material, respectively, and uniformly sprayed and inoculated into the Rhodiolae Crenulatae solid-state fermentation material, which was then fermented at 28°C for 4 days. After the fermentation was completed, the fermentation product was crushed and sieved through an 80 mesh sieve, and then subjected to supercritical fluid extraction in a supercritical fluid extraction kettle using 90% ethanol as a carrier (30% of the amount of the original material) at a pressure of 30 MPa, a temperature of 50°C, and a time of 2 h. After the extraction was completed, the oil and fat part was separated at a separation pressure of 5.2 MPa, and then concentrated at a reduced pressure at 40°C until no alcohol smell remained. The Rhodiolae Crenulatae essential oil obtained was labeled as Rhodiolae Crenulatae essential oil No. 1.

[0037] Example 2 Preparation of Rhodiolae Crenulatae Essential Oil Take 500 g of washed and dried rhodiola root and stem, crush it through an 80 mesh sieve to obtain rhodiola powder; mix the rhodiola powder with deionized water at a mass ratio of 1:1, sterilize at 121℃ for 20 min, and then take it out after cooling to obtain a solid-state fermentation material; mix 1wt% of the solid-state fermentation material with a Kluyveromyces lactis bacterial liquid with a viable bacterial count of 1×10 7 CFU / mL and 3wt% of Trichoderma asperellum spore liquid with a spore count of 1×10 6 CFU / mL, evenly spray and inoculate into the rhodiola solid-state fermentation material, and ferment at 28℃ for 3 days. After the fermentation is completed, crush the fermentation product and pass it through an 80 mesh sieve, and then place it in a supercritical fluid extraction kettle to perform supercritical fluid extraction with 90% ethanol as the entraining agent (the amount of which is 30% of the amount of the raw material). The extraction conditions are: pressure 30 MPa, temperature 50℃, and time 2 h. After the extraction is completed, separate the oil part, the separation pressure is 5.2 MPa, and the oil part is concentrated under reduced pressure at 40℃ until no alcohol smell remains. The rhodiola essential oil obtained by preparation is marked as rhodiola essential oil No. 2.

[0038] Application Examples 1-5 Using the plant essential oils prepared as described above, cosmetic samples are prepared according to the formulations in Table 1, and the sample preparation method includes the following steps: S1. According to Table 1, weigh the corresponding weight fractions of lavender essential oil, sweet orange essential oil, rosemary essential oil, rhodiola essential oil, ceramide NP, and sodium hyaluronate; S2. Homogeneously mix the lavender essential oil, sweet orange essential oil, rosemary essential oil, rhodiola essential oil, and 2wt% of PEG-40 hydrogenated castor oil based on the total amount of the cosmetic sample to obtain an oil phase; S3. Slowly add the sodium hyaluronate to the remaining deionized water, and homogeneously mix while heating and stirring to obtain a sodium hyaluronate solution; S4. After the sodium hyaluronate solution cools to room temperature, add the oil phase obtained in step S1, in which the PEG-40 hydrogenated castor oil enables the essential oil components and ceramide NP to be stably mixed with the water phase, and homogeneously mix the above components to prepare the cosmetic sample.

[0039] Table 1: Component amounts for preparing cosmetic samples

[0040] Note: “-” represents no addition; and the total mass of the efficacy composition is the same in different application examples.

[0041] Comparative Example 1 and Comparative Application Example 1 Preparation of the rhodiola essential oil: 500 g of washed and dried rhodiola root stems were pulverized through an 80-mesh sieve to obtain rhodiola powder; the rhodiola powder was placed in a supercritical fluid extraction kettle, and 90% ethanol was used as the entraining agent (30% of the amount of the raw material) to perform supercritical fluid extraction. The extraction conditions were: pressure 30 MPa, temperature 50°C, and time 2 h. After the extraction was completed, the oil and fat part was separated at a separation pressure of 5.2 MPa, and the oil and fat part was concentrated under reduced pressure at 40°C until no alcohol smell remained. The rhodiola essential oil obtained by the preparation was named Comparative Example 1.

[0042] A cosmetic sample was obtained according to the formula and preparation method of Application Example 2, and was named Comparative Application Example 1.

[0043] Comparative Example 2 and Comparative Application Example 2 The difference from Example 1 is that 6wt% of the active bacteria number of 1×10 8 CFU / mL of Kluyveromyces lactis liquid was uniformly sprayed and inoculated in the solid-state fermentation material to perform fermentation after the extraction of the essential oil, and the remaining steps and parameters were the same as the preparation method of the rhodiola essential oil of Example 1. The rhodiola essential oil obtained by the preparation was named Comparative Example 2.

[0044] A cosmetic sample was obtained according to the formula and preparation method of Application Example 2, and was named Comparative Application Example 2.

[0045] Comparative Example 3 and Comparative Application Example 3 The difference from Example 1 is that 6wt% of the active bacteria number of 1×10 7 CFU / mL of Trichoderma asperellum liquid was uniformly sprayed and inoculated in the solid-state fermentation material to perform fermentation after the extraction of the essential oil, and the remaining steps and parameters were the same as the preparation method of the rhodiola essential oil of Example 1. The rhodiola essential oil obtained by the preparation was named Comparative Example 3.

[0046] A cosmetic sample was obtained according to the formula and preparation method of Application Example 2, and was named Comparative Application Example 3.

[0047] Comparative Example 4 and Comparative Application Example 4 The difference from Example 1 is that 2wt% of the active bacteria number of 1×10 8 CFU / mL of Kluyveromyces lactis liquid (purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number: B336121) and 4wt% of the spore number of 1×10 7 CFU / mL of Trichoderma asperellum liquid were uniformly mixed and inoculated in the rhodiola solid-state fermentation material to perform fermentation after the extraction of the essential oil, and the remaining steps and parameters were the same as the preparation method of the rhodiola essential oil of Example 1. The rhodiola essential oil obtained by the preparation was named Comparative Example 4.

[0048] A cosmetic sample was obtained based on the formulation and preparation method of Application Example 2 and named Comparative Application Example 4.

[0049] Comparative Example 5 and Comparative Application Example 5 The difference from Example 1 is that: 2 wt% of the solid fermentation material contains 1 × 10⁻⁶ viable bacteria. 8 The cFU / mL Kluyveromyces lactis culture and the spore count of 4 wt% of the solid fermentation substrate were 1×10⁻⁶. 7 A solution of *Trichoderma echinosporum* (purchased from Ningbo Mingzhou Biotechnology Co., Ltd., product number: B94847) was mixed evenly and sprayed uniformly onto the *Rhodiola rosea* solid fermentation substrate for fermentation and extraction of essential oil. The remaining steps and parameters were the same as those in Example 1 for preparing *Rhodiola rosea* essential oil. The obtained *Rhodiola rosea* essential oil was named Comparative Example 5.

[0050] A cosmetic sample was obtained based on the formulation and preparation method of Application Example 2 and named Comparative Application Example 5.

[0051] Calculation of Rhodiola Rosea essential oil extraction rate: The extraction rates of Rhodiola rosea essential oil in Examples 1-2 and Comparative Examples 1-5 were calculated using the formula: (essential oil mass (g) / raw material mass (g)) × 100%. The extraction rates of Rhodiola rosea essential oil are as follows: Figure 1 As shown, the results indicate that the extraction rate of Rhodiola rosea essential oil was increased by 59.30%-68.60% after mixed fermentation with Kluyveromyces lactis and Trichoderma echinococcus, as specified in the specific accession number of this invention, compared with direct extraction without fermentation. Furthermore, a comparison of Example 1 with Comparative Examples 2-5 shows that mixed fermentation and fermentation with specific strains are more beneficial for improving the extraction rate of Rhodiola rosea essential oil. Example 1 showed an increase of 28.32%-57.61% compared to Comparative Examples 2-5, indicating that mixed fermentation of Rhodiola rosea with Kluyveromyces lactis (accession number CCTCC NO: M 20211628) and Trichoderma echinococcus (accession number CGMCC No. 16097) has a synergistic effect on improving the extraction rate of Rhodiola rosea essential oil.

[0052] Compare and contrast with example 6-12 Using the lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil obtained in the aforementioned preparations of this invention, and the rhodiola essential oil prepared in Example 1, cosmetic samples were prepared according to Table 2. The preparation methods are the same as in Application Example 2. Centella asiatica essential oil and sandalwood essential oil were both commercially available.

[0053] Table 2. Component dosage of comparative cosmetic samples

[0054] Note: "-" represents no addition; and the total mass of the efficacy composition in different comparative application examples is the same.

[0055] Test Example 1 Anti-inflammatory effect test The cosmetic samples prepared using application examples 1-5 and comparative application examples 1-12 were used as test samples, and their effects on the inflammatory factor TNF-α of human keratinocytes (HaCaT cells) were determined, and the experimental method was as follows: The HaCaT cells were cultured at 37°C, 5% CO2 environment with DMEM cell culture medium, and after 48h, the culture medium was replaced, and the cells were cultured until the cell confluence reached more than 80%, and then they were divided into the following groups: (1) Blank group: remove the culture medium, add culture buffer DPBS, and place in an environment with a UVB radiation dose of 0 J / cm2; after completion, remove DPBS and replace with DMEM culture medium, and culture in an incubator (37°C, 5% CO2) for 24h; (2) Model group: remove the culture medium, add culture buffer DPBS, and receive a total dose of 60mJ / cm2 UVB irradiation; after completion, remove DPBS and replace with DMEM culture medium, and culture in an incubator (37°C, 5% CO2) for 24h; (3) Experimental group: remove the culture medium, add culture buffer DPBS, and receive a total dose of 60mJ / cm2 UVB irradiation, and after completion, remove DPBS and replace with DMEM culture medium containing 500μg / mL of the corresponding test sample, and culture in an incubator (37°C, 5% CO2) for 24h; (4) Positive control group: remove the culture medium, add culture buffer DPBS, and receive a total dose of 60mJ / cm2 UVB irradiation, and after completion, remove DPBS and replace with culture medium containing 10μg / mL dexamethasone, and culture in an incubator (37°C, 5% CO2) for 24h. After the culture was completed, the supernatant was taken, and the content of inflammatory factor TNF-α in the supernatant was detected using an ELISA kit (purchased from Shengong Bioengineering (Shanghai) Co., Ltd.), and the content of TNF-α was calculated according to the kit method, and 3 replicate wells were set for each group.

[0056]

[0057] ​​​​​​​​​The results are shown in Table 3. Compared with the control group, the TNF-α content in the model group was significantly increased, indicating that UVB radiation caused an inflammatory response in HaCaT cells. The data from the examples show that the cosmetic sample provided by this invention can significantly reduce the TNF-α content in HaCaT cells, indicating that the efficacy composition provided by this invention has a good anti-inflammatory effect and a good repair effect on skin damage caused by photoaging. 1) The results of comparing application examples 1-5 with application example 2 show that Rhodiola rosea essential oil fermented by microorganisms can improve the anti-inflammatory effect of the composition, while Rhodiola rosea essential oil fermented with specific strains can further improve its anti-inflammatory effect. It is evident that even with the same strain, different strains used in mixed fermentation techniques have different effects. 2) Comparison of Application Examples 6-8 and 11-12 with Application Example 2 shows that omitting Rhodiola rosea essential oil or replacing it with Centella asiatica and sandalwood essential oils, which also have anti-inflammatory and repairing abilities, significantly reduces the anti-inflammatory efficacy of the composition. This indicates that in the composition described in this application, Rhodiola rosea essential oil can produce a synergistic effect with lavender, sweet orange, chamomile, and rosemary essential oils, and the ratio of different components also affects the anti-inflammatory effect of the composition. 3) Comparison of Application Examples 9-10 with Application Example 2 shows that the synergistic combination of ceramide NP and sodium hyaluronate also enhances the anti-inflammatory effect of the composition. The above results indicate that the composition provided by this invention, using a specific combination of compound plant essential oils, ceramide NP, and sodium hyaluronate, can significantly enhance the anti-inflammatory effect of the composition.

[0058] Table 3. TNF-α content in HaCaT cells Group TNF-α content (ng / L) Group TNF-α content (ng / L) Blank group 41.53±1.25 Comparative application example 1 59.92 ± 0.89 #* ]] Model group 65.81 ± 2.01 ▲ ]] Comparative application example 2 58.84 ± 1.23 #* ]] Application example 1 50.06 ± 1.28 # ]] Comparative application example 3 57.42 ± 1.26 #* ]] Application example 2 49.40 ± 1.01 # ]] Comparative application example 4 58.26 ± 1.18 #* ]] Application example 3 50.69 ± 1.03 # ]] Comparative application example 5 55.68 ± 1.35 #* ]] Application example 4 52.26 ± 0.68 # ]] Comparative application example 6 62.70 ± 0.69 #* ]] Application example 5 51.13 ± 0.95 # ]] Comparative application example 7 61.73 ± 1.54 #* ]] Positive control group 46.31 ± 1.59 # ]]> Comparative application example 8 60.01 ± 1.67 #* ]] Comparative application example 9 56.17 ± 1.12 #* ]] Comparative application example 10 57.48 ± 0.65 #* ]] Comparative application example 11 58.29 ± 0.89 #* ]] Comparative application example 12 59.88 ± 1.46 #* ]]

[0059] Note: In Table 3, ▲ This indicates that compared to the control group, p < 0.05; # This indicates that, compared with the model group, each application case and positive control group showed p < 0.05; * This indicates that, compared to Application Example 2, p < 0.05.

[0060] Test Example 2: Skin Irritation Test The cosmetic samples prepared using Application Examples 1-5 and Comparative Application Examples 1-12 were used as test samples. 170 people aged 16-65 years were randomly selected for the experiment. The test samples were subjected to a closed patch test for 0.5 hours according to the "Cosmetic Safety Technical Specifications (2015 Edition)". After removing the patch for 0.5 hours, the irritation was judged according to Table 4. The total score was the sum of individual scores / number of people.

[0061] Table 4. Grading Criteria for Skin Reaction in Occlusive Patch Tests Reaction degree Score level Skin reaction - 0 Negative reaction ± 1 Suspected reaction, only weak erythema + 2 Weak positive reaction, erythema, infiltration, edema, and papules can be present ++ 3 Strong positive reaction, erythema, infiltration, edema, papules, and vesicular reactions can exceed the test area +++ 4 Very strong positive reaction; obvious erythema, severe infiltration, edema, and confluent vesicles; reactions exceeding the test area

[0062] The skin reactions of all the subjects were negative after the test, and thus the results showed that the samples provided by the present application were all mild to the skin and safe to use.

[0063] Test Example 3: Test of skin repair efficacy and sleep aid effect Test sample preparation: equal amounts of the cosmetic samples prepared in Application Examples 1-5 and Comparative Application Examples 1-12 were taken, and 0.5% preservative (phenoxyethanol) was added based on the quality of the cosmetic to prepare test samples that could be used for short-term stability.

[0064] Volunteer selection: 170 women aged 28-50 years old who had difficulty falling asleep every day, or had dreamy sleep and were easily awakened, and whose skin had barrier damage were selected as volunteers for the test, and were divided into 17 groups, 10 people in each group, and were tested using the test samples prepared in the above application examples or comparative application examples.

[0065] (1) Skin repair effect determination: the volunteers applied 0.5 mL of the above test sample to the face before sleep and after cleansing in the morning every day for 4 weeks, and no other skin care products were used in the test area during the test period; and the following indexes were determined: a) Trans-epidermal water loss: trans-epidermal water loss (TEWL) is a commonly used index to reflect the barrier function of the stratum corneum, and can be used to evaluate the skin barrier function. The higher the TEWL value, the more water is lost through the skin, and the stratum corneum has poorer barrier function. Test method: the trans-epidermal water loss TEWL of the cheek test area before use and after 4 weeks of use was tested using a TEWL trans-epidermal water loss meter (Germany, Tewameter® TM 300), and the improvement rate of skin trans-epidermal water loss was calculated, and the calculation formula was: TEWL improvement rate (%) = [(TEWL measurement value of the test skin area before use-TEWL measurement value of the test skin area after 4 weeks of use) / TEWL measurement value of the test skin area before use] x 100%.

[0066] b) Lactic acid pricking score: Lactic acid pricking can be used to evaluate the sensitive state of the skin, and the improvement degree of the sensitive state of the skin can reflect the repair effect of the skin barrier function. Determination method: Before and after 4 weeks of use, the subjects clean the face and wipe it dry, then sit in a constant temperature and humidity chamber for 30 minutes, then apply 50 μL of 10% lactic acid aqueous solution to the nasal labial fold on one side of the cheek, and evaluate the itching, pricking, and burning discomfort of the test site by the subjects at 30 s, 2.5 min, and 5 min, respectively. The evaluation method is as follows: 0 points for no feeling, 1 point for mild, 2 points for moderate, and 3 points for severe. The evaluation scores at 0.5 min, 2.5 min, and 5 min are accumulated to obtain the lactic acid pricking score. The lactic acid pricking improvement rate is calculated, and the calculation formula is: lactic acid pricking improvement rate (%) = [(lactic acid pricking score before use - lactic acid pricking score after 4 weeks of use) / lactic acid pricking score before use] x 100%.

[0067] (2) Sleep aid effect determination: The subjective sleep quality of each test sample was evaluated according to the Pittsburgh Sleep Quality Index (PSQI) scale: 19 self-evaluation items and 5 other-evaluation items, of which the 19th self-evaluation item and the 5 other-evaluation items were not scored. The remaining 18 self-evaluation items were scored, which consisted of 7 dimensions: subjective sleep quality, sleep onset time, sleep time, sleep efficiency, sleep disorder, sleep aid drug use, and daytime dysfunction. Each dimension was scored on a 0-3 scale, and the cumulative score of each dimension was the PSQI total score, which was in the range of 0-21, and the higher the score, the worse the sleep quality. Specifically, a score > 7 indicates poor sleep quality, and a score ≤ 7 indicates good sleep quality. When the score decreases by ≥ 3 before and after testing, it is considered that the subjective sleep has improved.

[0068] Test results: (1) Skin repair effect: The determination results are shown in Table 5. From the transdermal water loss improvement rate and lactic acid pricking improvement rate results in the application examples, it can be known that the cosmetic prepared by the composition provided by the application has a significant moisturizing and repairing skin barrier effect. By comparing the transdermal water loss improvement rate and lactic acid pricking improvement rate results of the cosmetic samples of the comparative application examples 1-12 with those of the application examples, it can be known that the components in the efficacy composition provided by the application have a synergistic effect, which can synergistically improve the skin moisturizing and repairing effect of the efficacy composition. At the same time, by comparing the application example 2 and the comparative application examples 1-5, it can be known that the rhodiolae essential oil fermented by the mixed strains can improve the skin moisturizing and repairing effect of the efficacy composition, and the rhodiolae essential oil fermented by the specific mixed strains can further improve the skin moisturizing and repairing effect of the efficacy composition.

[0069] Table 5 Transdermal water loss improvement rate and lactic acid pricking improvement rate results Group Improvement rate of transdermal water loss Improvement rate of lactic acid stinging Application example 1 24.31% 53.18% Application example 2 25.57% 55.62% Application example 3 24.68% 51.34% Application example 4 23.19% 50.42% Application example 5 24.91% 53.39% Comparative application example 1 16.51% 33.41% Comparative application example 2 18.05% 39.16% Comparative application example 3 17.53% 37.24% Comparative application example 4 19.08% 40.58% Comparative application example 5 20.19% 42.30% Comparative application example 6 14.81% 28.96% Comparative application example 7 18.35% 37.38% Comparative application example 8 17.87% 36.57% Comparative application example 9 19.56% 40.69% Comparative application example 10 17.85% 35.98% Comparative application example 11 18.93% 38.83% Comparative application example 12 18.64% 36.47%

[0070] (2) Sleep aid effect: The test results are shown in Table 6, and the results show that, from the results of the examples, the cosmetic prepared from the composition provided by the application has good sleep aid effect and can significantly improve the sleep of the subjects.

[0071] Table 6 Sleep improvement test results Group PSQI score before use QSPI score after 4 weeks of use Score decrease Application example 1 12.8 8.0 4.8 Application example 2 12.6 7.5 5.1 Application example 3 13.0 8.4 4.6 Application example 4 12.5 8.2 4.3 Application example 5 12.0 7.1 4.9 Comparative application example 1 13.0 10.5 2.5 Comparative application example 2 13.5 10.7 2.8 Comparative application example 3 14.1 11.2 2.9 Comparative application example 4 13.3 10.6 2.7 Comparative application example 5 12.7 9.4 3.3 Comparative application example 6 12.9 10.5 2.4 Comparative application example 7 13.1 10.1 3.0 Comparative application example 8 14.0 10.8 3.2 Comparative application example 9 13.4 10.5 2.9 Comparative application example 10 13.7 10.7 3.0 Comparative application example 11 12.8 10.3 2.5 Comparative application example 12 13.6 11.0 2.6

[0072] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present application and not to limit the scope of protection of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present application.

Claims

1. A composition having sleep-aiding and skin-soothing efficacy, characterized in that, The composition comprises the following components in parts by weight: compound plant essential oil 72-114 parts, ceramide NP 3-5 parts, and sodium hyaluronate 1-3 parts; the compound plant essential oil is composed of lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil, and rhodiola essential oil in a mass ratio of (20-35):(16-25):(20-30):(6-9):(10-15); the rhodiola essential oil is obtained by fermenting rhodiola with mixed bacteria and extracting essential oil from the rhodiola fermentation product; the mixed bacteria are Kluyveromyces lactis and Trichoderma asperellum.

2. The composition of claim 1, wherein, The composition comprises the following components in parts by weight: compound plant essential oil 93-114 parts, ceramide NP 4-5 parts, and sodium hyaluronate 2-3 parts.

3. The composition of claim 1, wherein The compound plant essential oil is composed of lavender essential oil, sweet orange essential oil, chamomile essential oil, rosemary essential oil, and rhodiola essential oil in a mass ratio of (28-35):(20-25):(25-30):(7.5-9):(12.5-15).

4. The composition of claim 1, wherein The fermentation conditions are: inoculating 1-2% of a lactic acid Kluyveromyces liquid with a viable bacterial count of 10 7 -10 9 CFU / mL and 3-5% of a Trichoderma spinosum liquid with a spore count of 10 6 -10 7 CFU / mL at 25-30°C for 3-5 days.

5. Use of the composition with sleep-aiding and skin-protection effects according to any one of claims 1-4 in the preparation of sleep-aiding and skin-protection cosmetics.

6. The use according to claim 5, characterized in that, The mass percentage of the composition with sleep-aiding and skin-protection effects is 0.1-3%.

7. The use according to claim 5, wherein the compound is ###00003### 5 The dosage form of the cosmetic includes any one of cream, lotion, emulsion, and oil.

8. A sleep-aiding, restorative cosmetic product, characterized by, The mass percentage of the composition with sleep-aiding and skin-protection effects is 0.1-3%.

9. The sleep-aiding repair cosmetic product according to claim 8, wherein The cosmetic also contains adjuvants acceptable in the cosmetic field.

10. The sleep-aiding repair cosmetic product according to claim 9, wherein The adjuvant includes any one or several of emulsifiers, humectants, emollients, pH regulators, penetration enhancers, preservatives, and deionized water. The adjuvant includes any one or several of emulsifiers, humectants, emollients, pH regulators, penetration enhancers, preservatives, and deionized water.

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