The invention relates to a vacuum pumping device for an interlayer of a cryogenic insulation cylinder, which belongs to the technical field of cryogenic insulation vacuum. The vacuum pumping device is mainly technically characterized by comprising the cryogenic insulation cylinder, a heating oven, an internal air circulating fan, a combustion machine, a heating branch pipe, an exhaust branch pipe, an internal heating main pipe, an external exhaust main pipe, a nitrogen heating device, a nitrogen displacement valve, a pneumatic flapper valve I, a pneumatic flapper valve II, a diffusion pump, a pneumatic flapper valve III, a rotary vane pump and a Roots pump, wherein the internal air circulating fan is used for introducing heated high-temperature air into the interlayer of the cylinder. The two surfaces of an outer cylinder body and an inner liner are heated simultaneously, so that hydrogen, helium and other low molecular weight gas molecules, water molecules and the like, which are adsorbed in a heat insulation material in the interlayer, can be activated, the gas release speed of the interlayer is enhanced, and the vacuum pumping speed of the interlayer is effectively improved. The vacuum pumping device has the advantages of reasonable structure, safety and convenience in use, high vacuum pumping degree, no re-bounce of vacuum degree, long holding time and the like.