Mineralization guided tissue regeneration membrane and preparation method and application thereof

A technology to guide tissue regeneration and mineralize collagen, applied in medical science, prosthesis, etc., can solve problems such as poor regeneration function, and achieve good tissue compatibility, moderate thickness, and low immunogenicity

Active Publication Date: 2017-03-15
BEIJING ALLGENS MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The technical problem to be solved by the present invention is to provide a mineralization-guiding tissue regeneration membrane and its preparation method and application for the defect that the pure collagen membrane has a weak function of guiding tissue regeneration

Method used

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  • Mineralization guided tissue regeneration membrane and preparation method and application thereof
  • Mineralization guided tissue regeneration membrane and preparation method and application thereof
  • Mineralization guided tissue regeneration membrane and preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0055] see figure 1 , is a flowchart of a method for preparing a mineralization-guided tissue regeneration membrane according to a preferred embodiment of the present invention. Such as figure 1 Shown, this preparation method comprises the following steps:

[0056] Step S1, preparation of mineralized collagen particles, specifically includes:

[0057] Step S1-1, dissolving collagen in any one of hydrochloric acid, nitric acid or acetic acid to prepare an acid solution of collagen, wherein the concentration of collagen is 0.01-0.5 g / ml (for example, 0.01, 0.1, 0.2 or 0.5 g / ml) . The collagens used in the present invention are all type I collagens.

[0058] Step S1-2, adding calcium salt solution dropwise to the collagen acid solution, wherein the amount of calcium ions added is 0.1-2 mol (eg 0.1, 0.5, 1, 1.5 or 2 mol) of calcium ions per gram of collagen. More preferably, 1-2 mol of calcium ions are added per gram of collagen.

[0059] Step S1-3, adding phosphoric acid so...

Embodiment 1

[0092] (1) Dissolving type Ⅰ collagen in acetic acid with a concentration of 0.5M to form an acid solution of collagen, wherein the concentration of type Ⅰ collagen is 0.01g / ml;

[0093] (2) adding a calcium salt solution dropwise to the acid solution of the collagen, wherein the amount of calcium ions added is 1 mol of calcium ions corresponding to each gram of collagen;

[0094] (3) Add phosphoric acid solution dropwise in the solution obtained in step 2, wherein the molar ratio of the amount of phosphate ions added to the amount of calcium ions added in step S1-2 is Ca / P=1.5 / 1;

[0095] (4) NaOH solution is added dropwise in the solution obtained in step 3 to form a mixed solution, and the pH value is adjusted to be 8;

[0096] (5) After the mixed solution obtained in step 4 was left to stand for 4 hours, the precipitate was centrifuged at a speed of 3000 r / min and then air-dried at 50° C. for 24 hours to obtain mineralized collagen particles.

[0097] (6) dissolving colla...

Embodiment 2 to 14

[0105] Except for the contents of Table 1 and Table 2 below, it was carried out in substantially the same manner as in Example 1.

[0106]

[0107]

[0108]

[0109] Note: The collagen concentration in the second collagen aqueous solution represents the mass concentration of collagen.

[0110] SEM result observation

[0111] The mineralization-guided tissue regeneration membrane prepared in Example 1 was observed by a scanning electron microscope (SEM). see image 3 is the SEM result map of the loose layer, Figure 4 is the SEM result map of the dense layer, Figure 5 It is the SEM result picture of the section of the mineralization-guided tissue regeneration membrane. As shown in the figure, the loose layer has a porous structure, while the dense layer has a smooth surface. From the cross-sectional views of the two, it can be seen that the dense layer is composed of type I collagen fiber bundles closely arranged, and the loose layer is compounded with nano-hydrox...

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Abstract

The invention relates to a mineralization guided tissue regeneration membrane and a preparation method and application thereof, wherein the mineralization guided tissue regeneration membrane comprises: a loose layer formed by compositing type I collagen and nano hydroxyapatite; a compact layer positioned on the loose layer and formed from type collagen. The double-layer mineralization guided tissue regeneration membrane is constructed herein, wherein the loose layer is consistent to autogenous bone in terms of composition, and the membrane may be attached to diseased oral bone defect surface to induce the generation of new bones; the surface of the compact layer is smooth, a diseased area may be isolated from surrounding tissues by making good use of physical barrier function of the membrane, regenerating capacity of a specific tissue is given to maximum play, and the prepared mineralization guided tissue regeneration membrane has high tensile strength and elasticity.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a mineralization-guided tissue regeneration membrane, a preparation method and application thereof. Background technique [0002] Periodontal disease is one of the three major non-communicable diseases that endanger human health. It refers to diseases that occur in the tooth supporting tissue (periodontal tissue), including gingival disease that only involves gingival tissue and deep periodontal tissue (periodontal ligament). , alveolar bone, cementum) of periodontitis two categories. The treatment of periodontal disease includes three aspects: elimination of inflammation, prevention of recurrence of infection and reconstruction of normal structure and function of periodontal tissue. Traditional treatment methods can only eliminate inflammation and control the development of lesions, but the damaged periodontal tissue is difficult to restore to normal. Studies have found tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/24A61L27/12
CPCA61L27/12A61L27/24
Inventor 胡艳丽宋天喜张华崔云仇志烨崔菡崔福斋
Owner BEIJING ALLGENS MEDICAL SCI & TECH
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