Method for simultaneous determination of 92 antibacterial drug residues in water environment

A technology for antibacterial drugs and detection methods, which is applied in the field of detection of antibacterial drug residues in water environments, can solve problems such as time-consuming, high detection costs, and cumbersome methods, and achieve the effects of simple operation, high sensitivity, and improved detection efficiency

Active Publication Date: 2017-08-08
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no relevant reports on detection methods for 8 categories of 92 antimicrobial drug residues
At the same time, the existing methods are cumbersome, time-consuming, and the detection cost is relatively high.

Method used

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  • Method for simultaneous determination of 92 antibacterial drug residues in water environment
  • Method for simultaneous determination of 92 antibacterial drug residues in water environment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the mensuration of 92 kinds of antibacterial drugs content in water environment

[0036] (1) Water sample pretreatment

[0037] The water sample is filtered with a 0.45 μm filter membrane, and 100 mL of water sample is taken, and the chelating agent Na 2 EDTA, adjust pH to 8.0.

[0038] (2) Enrichment and purification by solid phase extraction

[0039] Use 3mL methanol and 3mL water to activate the solid-phase extraction column, add the water sample to be enriched, control the flow rate of the water sample (1.0mL / min), use water to rinse the solid-phase extraction column, and use 6mL methanol:water=90:10 , The solid-phase extraction column was eluted with the elution solvent in V / V, and dried with nitrogen at 40°C. On the basis of V / V, the residue was dissolved with methanol: water = 10:90, and the volume was adjusted to 1 mL, and detected by liquid chromatography-tandem mass spectrometry.

[0040] (3) Liquid chromatography-tandem mass spectrometry det...

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Abstract

The invention belongs to the technical field of determination of antibacterial drug residues in water environment, and in particular relates to a method for simultaneous determination of 92 antibacterial drug residues in the water environment. The method includes an improved solid phase extraction-liquid chromatography-tandem mass spectrometry determination method, and in particular includes (1) water sample pretreatment; (2) use of solid phase extraction for enrichment of various antibacterial drugs in a water sample; and (3) liquid chromatography-tandem mass spectrometry determination method establishment. The sample is fed by one time, can simultaneously determine 92 antibacterial drugs, reduces the detection cost, and improves detection efficiency, and is suitable for the detection of antibacterial drug residues in high flux water environment.

Description

technical field [0001] The invention belongs to the technical field of antibacterial drug residue detection in water environment. It specifically relates to a method for simultaneously detecting 92 kinds of antimicrobial drug residues in a water environment, and is related to the technical field of drug residue analysis. Background technique [0002] As the material basis for the development of modern animal husbandry, veterinary medicine has played an important role in reducing the morbidity and mortality of livestock and poultry, promoting animal growth, improving animal product quality and increasing feed utilization. Antimicrobial drugs are the most widely used veterinary drugs in my country, including antibiotics such as β-lactams, tetracyclines, chloramphenicol, macrolides, lincosamides, polypeptides and tiamulin and other antibiotics , Chemically synthesized antibacterial drugs such as sulfonamides and their synergists, quinolones, quinoxalines and nitroimidazoles and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 袁宗辉陈冬梅高金芳陶燕飞潘源虎谢书宇黄玲利彭大鹏刘振利
Owner HUAZHONG AGRI UNIV
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