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Injectable nano-short fibers loaded with anti-cancer medicine as well as preparation method and application thereof

An anticancer drug and short fiber technology, applied in the field of medicine, can solve problems such as low utilization rate, low drug efficacy, affecting research value and use value, and achieve easy operation, safe treatment, good blood compatibility and cell compatibility. capacitive effect

Active Publication Date: 2017-07-07
泉州威可赛机械科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, the drug-loaded fiber membranes with composite properties prepared by electrospinning technology are mostly used as medical materials for external application, with low drug efficacy and low utilization rate, which greatly affects their broad research value and use value.

Method used

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  • Injectable nano-short fibers loaded with anti-cancer medicine as well as preparation method and application thereof
  • Injectable nano-short fibers loaded with anti-cancer medicine as well as preparation method and application thereof
  • Injectable nano-short fibers loaded with anti-cancer medicine as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0046] This embodiment provides a method for preparing injectable short nanofibers loaded with anticancer drugs, which includes the following steps:

[0047] (1) Weigh DOX and MWNTs according to the mass ratio of 1:1, mix them fully and uniformly under ultrasonic conditions, then adjust the pH to 9.5, and fully react for 24 hours under stirring conditions to obtain the reaction product. First use sodium hydroxide with a pH of 9.5 The aqueous solution was washed 4 times, centrifuged at 8500rpm for 30min, and finally freeze-dried at -80°C for 72h to obtain DOX-loaded MWNTs powder; after testing, the drug-loading capacity of the DOX-loaded MWNTs powder was 99.6wt%, encapsulation rate 49.9wt%;

[0048] Wherein, the MWNTs are prepared by the following method: using LaNiO 3 Disperse it into a porcelain boat as a catalyst, put the porcelain boat in a quartz tube, and place it in the constant temperature zone of the tube furnace, vacuumize, feed hydrogen, the pressure is normal press...

Embodiment 2

[0053] This embodiment provides a method for preparing injectable short nanofibers loaded with anticancer drugs, which includes the following steps:

[0054] (1) Weigh DOX and MWNTs respectively according to the mass ratio of 1:2, fully and uniformly mix under ultrasonic conditions, then adjust the pH to 10, and fully react for 48 hours under stirring conditions to obtain the reaction product. First use sodium hydroxide with a pH of 10 The aqueous solution was washed 3 times, centrifuged at 8000rpm for 25min, and finally freeze-dried at -75°C for 48h to obtain DOX-loaded MWNTs powder; after testing, the drug-loading capacity of the DOX-loaded MWNTs powder was 95.4wt%, encapsulation rate 48.2wt%;

[0055] Wherein, the MWNTs are prepared by the following method: using LaNiO 3 Disperse it into a porcelain boat as a catalyst, put the porcelain boat in a quartz tube, and place it in the constant temperature zone of the tube furnace, vacuumize, feed hydrogen, the pressure is normal...

Embodiment 3

[0060] This embodiment provides a method for preparing injectable short nanofibers loaded with anticancer drugs, which includes the following steps:

[0061] (1) Weigh DOX and MWNTs respectively according to the mass ratio of 2:1, carry out sufficient and uniform mixing under ultrasonic conditions, then adjust the pH to 9.5, and carry out a full reaction for 36 hours under stirring conditions, to obtain the reaction product, first use a NaOH aqueous solution with a pH of 9.5 ( Prepared by mixing 1mol / mL HCL and NaOH) and washed 5 times, then centrifuged at 8500rpm for 20min, and finally freeze-dried at -80°C for 72h to obtain DOX-loaded MWNTs powder; after testing, the The drug loading of the DOX-loaded MWNTs powder is 96.1wt%, and the encapsulation rate is 48.9wt%;

[0062] Wherein, the MWNTs are prepared by the following method: using LaNiO 3 Disperse it into a porcelain boat as a catalyst, put the porcelain boat in a quartz tube, and place it in the constant temperature zo...

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Abstract

The invention relates to a preparation method of injectable nano-short fibers loaded with an anti-cancer medicine. The preparation method comprises the following steps: loading the anti-cancer medicine DOX (Doxycycline) on MWNTs (multi-walled carbon nanotubes) with a hollow structure to obtain MWNTs powder loaded with the DOX; carrying out electrostatic spinning on the MWNTs powder loaded with the DOX and biodegradable macromolecular PLGA (poly(lactic-co-glycolic acid)) under a specific condition to obtain a compound nano-fiber membrane; finally, carrying out crushing and homogenization treatment on the compound nano-fiber membrane to prepare the injectable nano-short fibers loaded with the anti-cancer medicine. The injectable nano-short fibers loaded with the anti-cancer medicine have a good releasing performance, can be used for injecting, has high medicine utilization rate and high stability and can be used for more accurately, effectively and safely treating tumor parts; the injectable nano-short fibers loaded with the anti-cancer medicine have good blood compatibility and cell compatibility, can be used for killing cancer cells and do not cause injuries to normal tissue cells of human bodies; the injectable nano-short fibers can emit two types of fluorescent light, are swallowed by human body cells and can be used for researching a cancer cell treatment mechanism.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to an injectable nano-short fiber DOX / MWNTs / PLGA loaded with anticancer drugs, a preparation method and application thereof. Background technique [0002] With the aggravation of environmental pollution and the development of people's bad habits, the incidence of cancer is getting higher and higher. At present, the treatment methods for cancer are still based on surgery, radiotherapy and chemotherapy. Surgery and radiotherapy are local treatments with certain limitations. Chemotherapy can treat the whole body through the release of drugs, and at the same time, it can also have a good effect on the potential metastasis and recurrence of cancer cells and advanced cancer cells. However, most chemotherapeutic drugs currently used clinically for cancer treatment show problems such as low water solubility, limited stability, rapid blood clearance, and lack of selectivity. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K47/02A61K47/34A61K31/704A61P35/00D01F6/92D01F1/10D01D5/00
CPCA61K9/0002A61K9/0019A61K9/0092A61K31/704A61K47/02A61K47/34D01D5/0015D01F1/103D01F6/92
Inventor 史向阳魏延传张振文李鑫尹迪徐刚伟王梦媛
Owner 泉州威可赛机械科技有限公司
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