High-throughput gene sequencing dynamic dispatching control method and system device

A technology of gene sequencing and dynamic scheduling, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of unsatisfactory sequencing results, high precision requirements, and high control requirements, and achieve the The effect of a large temperature-controlled reaction rate

Inactive Publication Date: 2015-09-09
XI AN JIAOTONG UNIV
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Problems solved by technology

The entire sequencing process requires high precision, and any slight error may lead to unsatisfactory sequencing results, and requires coordination and cooperation between various components, including the amount and type of reagents, reaction temperature, time, cleanliness, nanoscale displacement, focusing Adjustment, luminous intensity, optical path adjustment, exposure time calculation, image capture and other aspects of control require high requirements and are difficult

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  • High-throughput gene sequencing dynamic dispatching control method and system device
  • High-throughput gene sequencing dynamic dispatching control method and system device
  • High-throughput gene sequencing dynamic dispatching control method and system device

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Embodiment Construction

[0048] The implementation of the present invention will be described in detail below in conjunction with the drawings and examples.

[0049] The present invention uses a second-generation high-throughput gene sequencer, which can be divided into four modules: a flow cell carrier module; a bridge PCR module; a sequencing-by-synthesis module and a software-synthesis-sequencing module. The integrated control of the environment refers to the integration of the temperature and reagent control of the bridge PCR module and the reaction chamber of the sequencing-by-synthesis module.

[0050] The flow cell module is a place for storing DNA fragments to be tested and DNA synthesis and sequencing reactions in high-throughput gene sequencing. According to different models, flow cells can be divided into 2, 4, and 8-channel flow cells. This patent uses 2-channel flow groove. The inner surface of each channel can randomly immobilize single-stranded DNA fragments with adapters in the form o...

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Abstract

The invention relates to a high-throughput gene sequencing dynamic dispatching control method and system device. The system device comprises a temperature control system, a reagent control system, a laser trigger control system, a galvanometer scanning system and an optical acquisition system, wherein bridge-type PCR amplification is carried out for an interrupted to-be-tested fragment in a flow channel by virtue of the temperature and reagent control systems; the sequencing by synthesis (SBS) reaction is enabled to be successfully carried out by virtue of the temperature and reagent control systems; a fluorescent signal is captured and data is stored by virtue of laser triggering, galvanometer scanning and optical acquisition, so that an integrated control system of a high-throughput gene sequencing instrument is realized.

Description

technical field [0001] The invention belongs to the technical field of high-throughput gene sequencing, and in particular relates to a dynamic scheduling control method and system device for high-throughput gene sequencing. Background technique [0002] High-throughput gene sequencing technology involves interdisciplinary subjects such as mechanics, electronic communications, biology, chemistry, and optics. The entire sequencing process requires high precision, and any slight error may lead to unsatisfactory sequencing results, and requires coordination and cooperation between various components, including the amount and type of reagents, reaction temperature, time, cleanliness, nanoscale displacement, focusing Adjustment, luminous intensity, optical path adjustment, exposure time calculation, image capture and other aspects of control require high requirements and are difficult. Contents of the invention [0003] In order to overcome the above-mentioned shortcomings of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12Q3/00
CPCC12Q1/6869C12Q2563/107C12Q2535/122
Inventor 韩九强张朋刘俊吕红强钟德星
Owner XI AN JIAOTONG UNIV
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