Systems and methods using magnetically-responsive sensors for determining a genetic characteristic

A genetically characterized and responsive technology, applied in chemical instruments and methods, biochemical equipment and methods, and laboratory containers, can solve complex, bulky, expensive and other problems

Active Publication Date: 2018-06-08
ILLUMINA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Currently, next-generation sequencing (NGS) systems based on sequencing-by-synthesis (SBS) are complex, expensive, and cumbersome

Method used

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  • Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
  • Systems and methods using magnetically-responsive sensors for determining a genetic characteristic
  • Systems and methods using magnetically-responsive sensors for determining a genetic characteristic

Examples

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Embodiment Construction

[0308] The methods described herein can be used in conjunction with various biological or chemical analysis techniques including nucleic acid sequencing techniques. Embodiments may be used to determine the genetic characteristics of a sample based on the change in electrical resistance that occurs as a strand of nucleic acid grows. Particularly suitable techniques are those in which biological or chemical samples are located in designated positions such that their relative positions do not change during analysis. For example, nucleic acids can be attached to fixed locations along the substrate surface during a given protocol in which the array is repeatedly scanned. Embodiments are particularly suitable where different lanes are used to obtain imprints, for example, to coincide with different labels used to distinguish one nucleotide base type from another. In some embodiments, the process of determining the nucleotide sequence of a target nucleic acid can be an automated pro...

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Abstract

Sequencing-by-synthesis (SBS) method is provided that includes providing a detection apparatus that includes an array of magnetically-responsive sensors. Each of the magnetically-responsive sensors islocated proximate to a respective designated space to detect a magnetic property therefrom. The detection apparatus also includes a plurality of nucleic acid template strands located within corresponding designated spaces. The method also includes conducting a plurality of SBS events to grow a complementary strand by incorporating nucleotides along each template strand. At least some of the nucleotides are attached to corresponding magnetic particles having respective magnetic properties. Each of the plurality of SBS events includes detecting changes in electrical resistance at the magnetically-responsive sensors caused by the respective magnetic properties of the magnetic particles. The method also includes determining genetic characteristics of the complementary strands based on the detected changes in electrical resistance.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of identically titled US Provisional Application No. 62 / 205,336, filed August 14, 2015, which is hereby incorporated by reference in its entirety. Background of the invention [0003] Currently, next-generation sequencing (NGS) systems based on sequencing-by-synthesis (SBS) are complex, expensive, and cumbersome. Therefore, the SBS instrument expects new detection methods. Summary of the invention [0004] In an embodiment, there is provided a sequencing by synthesis (SBS) method comprising providing a detection device comprising an array of magnetically responsive sensors. Each magnetically responsive sensor is positioned adjacent to a corresponding designated space to detect magnetic properties from that space. The detection device also includes a plurality of nucleic acid template strands located in corresponding designated spaces. The method also includes performing multiple S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869C12Q1/6874G01R33/09G01N33/543G01N27/00
CPCC12Q1/6869C12Q1/6874G01N27/3276G01N33/543G01R33/093G01R33/098G01R33/1276G11C11/161B01L3/502761C12Q2563/143C12Q2565/607
Inventor J.G.曼德尔L.夸克
Owner ILLUMINA INC
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