Green sugar suitable for hyperglycemia patients and preparation method thereof

Abstract

The invention provides green sugar capable of effectively treating hyperglycemia and a preparation method of the green sugar. The green sugar comprises 10-20 parts of cyclocarya paliurus leaves, 5-8 parts of compatible medicines, 2-5 parts of a synergist, 5-7 parts of grape seeds and 1-2 parts of bitter gourds. Through reasonable compatibility, the green sugar is prepared, the green sugar has a very good effect of treating hyperglycemia, meanwhile, the raw materials are few, preparation is easy, storage is easy, taking is easy and convenient, hyperglycemia complications are effectively reduced, long-term taking can be achieved, mixed extraction of the cyclocarya paliurus leaves and compatible medicines can change the pH value, viscosity and the like in the mixed extraction process, and the green sugar can be used for treating hyperglycemia. The dissolution of effective components in the cyclocarya paliurus leaves is improved, the extraction efficiency is improved, meanwhile, the spleen tonifying effect of the Chinese yam can promote the absorption of the effective components in the cyclocarya paliurus leaves by human bodies, the defect of deficiency-cold in spleen and stomach possibly caused by the burdock roots is relieved, and meanwhile, the generation links and cost are reduced through mixed extraction.

Description

Technical Field

[0001] The invention relates to the field of traditional Chinese medicine combinations, in particular to green sugar suitable for patients with hyperglycemia and a preparation method thereof. Background Art

[0002] With the continuous improvement of people's living standards and changes in dietary structure, the number of patients with high blood sugar has increased year by year. Diabetes has become the "third killer" threatening humans after cardiovascular diseases and tumors. The prevention and treatment of diabetes has become a global problem. At present, there are many clinically effective drugs for lowering blood sugar, but they have large side effects on the human body. For example, bipolar drugs can cause intestinal discomfort and affect the absorption of vitamin B12; sulfonylureas can cause hypoglycemia and secondary failure; glinides need to be taken multiple times a day and are expensive; α-glucosidase inhibitors need to be taken with meals and are prone to intestinal discomfort; thiazolidinediones are prone to weight gain, edema and heart failure; DDP-4 is prone to headaches, upper respiratory tract infections and is expensive; LGP-1 receptor agonists need to be injected subcutaneously, which is inconvenient to use and is prone to intestinal discomfort; insulin needs to be injected subcutaneously, which is complicated to store and use, and there is a risk of hypoglycemia when used.

[0003] Since the above treatment options have various complications or are expensive and inconvenient to use when treating hyperglycemia, traditional Chinese medicine is becoming increasingly popular in treating hyperglycemia. Summary of the invention

[0004] In view of the problems existing in the prior art, the present invention discloses a Chinese medicine combination and a preparation method, specifically a green sugar suitable for patients with hyperglycemia and a preparation method, which can well control and lower blood sugar, and has low cost, easy storage, convenient use, few adverse reactions, can be taken for a long time, and is suitable for clinical promotion and application.

[0005] To achieve the above purpose, the technical solution provided by the present invention is as follows:

[0006] On the one hand, the present application provides a green sugar suitable for patients with hyperglycemia. The raw materials are composed of the following components by weight ratio: 10-20 parts of green willow leaves, 5-8 parts of compatible drugs, 2-5 parts of enhancers, 5-7 parts of grape seeds, and 1-2 parts of bitter melon.

[0007] As an improvement of the above technical solution, the combined medicine includes wolfberry, burdock root, yam and ginseng, and the weight ratio of wolfberry, burdock root, yam and ginseng is (7-9): (10-12): (8-12): (5-8).

[0008] As an improvement of the above technical solution, the synergist includes astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza, and the weight ratio of astragalus, kudzu root, coptis root, raw rehmannia and chuanxiong is (6-8): (6-8): (1-2): (4-6): (4-6).

[0009] In a second aspect, the present invention provides a method for preparing the green sugar, comprising the following steps:

[0010] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0011] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0012] S3: Weighing astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza according to weight ratio to prepare a synergist;

[0013] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0014] S5: adding grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mixing evenly, and pressing into sheets or blocks.

[0015] As an improvement of the above technical solution, the preparation method of grape seed extract in step S1 is as follows:

[0016] S11: Weigh high-quality grape seeds according to weight ratio;

[0017] S12: placing grape seeds into an extraction kettle;

[0018] S13: Introduce carbon dioxide gas into the equipment, adjust the pressure to 10-40Mpa, and the temperature to 30-60°C;

[0019] S14: stirring the carbon dioxide and grape seeds in the reaction kettle to allow them to fully contact and extract for 1-3 hours;

[0020] S15: the extracted extract enters a separation kettle along with the supercritical fluid, and the pressure and temperature are changed to convert the carbon dioxide into gas, thereby separating the grape seed extract;

[0021] S16: opening the separation kettle to collect the grape seed extract;

[0022] S17: Analyze the extract using high performance liquid chromatography to ensure that the extract meets the quality standards;

[0023] S18: Drying and collecting the grape seed extract.

[0024] As an improvement of the above technical solution, the preparation method of the bitter melon extract in step S2 is as follows:

[0025] S21: Weigh fresh bitter melon by weight, wash and slice;

[0026] S22: taking a 50-70% ethanol aqueous solution and placing it in an extraction container, and adding bitter melon slices, wherein the liquid-to-material ratio of the ethanol aqueous solution to the bitter melon slices is 5:1-20:1;

[0027] S23: The temperature is controlled at 40-80°C and the container is placed in an ultrasonic environment for 0.5-2 hours;

[0028] S24: centrifugation and filtration to separate the filtrate from the residue;

[0029] S25: The filtrate is subjected to chromatographic separation and purification, and concentrated to obtain the bitter melon extract.

[0030] As an improvement of the above technical solution, the preparation method of the synergist in step S3 is as follows:

[0031] S31: Weigh astragalus, kudzu root, coptis root, raw rehmannia root, salvia miltiorrhiza according to weight ratio, wash, dry, mix and grind;

[0032] S32: adding phosphate buffer to a container, adding crushed medicinal materials, wherein the ratio of phosphate buffer to medicinal materials is 5:1-10:1, stirring to prepare a medicinal material suspension;

[0033] S33: controlling the temperature at 40-60° C., adding cellulase, wherein the weight ratio of the cellulase to the medicinal material is 0.5-2%, stirring, and performing enzymolysis for 1-3 hours;

[0034] S34: reflux extraction of the enzymatically hydrolyzed medicinal material with 60-80% ethanol;

[0035] S35: filtering the medicinal residue to obtain an extract;

[0036] S36: spray-drying the extract to prepare powder to obtain a synergist.

[0037] As an improvement of the above technical solution, the preparation method of the mixed extract in step S4 is as follows:

[0038] S41: weighing cyclocarya paliurus leaves, wolfberry, burdock root, yam and ginseng according to weight ratio, washing, drying, mixing and crushing;

[0039] S42: adding water to a container, adding crushed medicinal materials, with a water to medicinal material ratio of 5:1-10:1, and soaking for 1-2 hours;

[0040] S43: After boiling, control the temperature to 80-100° C., stir, and extract for 1-2 hours;

[0041] S44: filtering the medicinal residue to obtain an extract;

[0042] S46: spray-drying the extract to make powder to obtain the mixed extract.

[0043] As an improvement of the above technical solution, the flake or block green sugar prepared in step S6 is stored in a cool and dry environment.

[0044] The invention provides a green sugar capable of effectively treating hyperglycemia. The green sugar can lower blood sugar and fundamentally solve the symptoms of patients with hyperglycemia. Meanwhile, the green sugar has few raw materials, low price, simple preparation process, simple and convenient medication for patients, and can be taken for a long time. The green poplar leaf of the invention has the effects of clearing away heat, relieving thirst and detoxifying, and contains multiple biologically active ingredients (flavonoids, triterpenes and polysaccharide compounds), has the effects of lowering blood sugar and blood lipid, and is therefore reused. Bitter melon, coptis root and burdock root can clear away heat and dampness, purge fire and detoxify, and have the effects of lowering blood sugar, and can strengthen the effects of clearing heat and lowering blood sugar together with the green poplar leaf. Lycium barbarum can nourish the liver and kidney; kudzu root can produce body fluid and quench thirst, relieve muscles and reduce fever; raw rehmannia root can clear away heat and cool blood, nourish yin and produce body fluid; astragalus and ginseng can replenish qi and raise yang, and enhance the body's immunity; yam can invigorate the spleen and stomach, and improve the digestive function and nutrient absorption of patients with hyperglycemia; salvia miltiorrhiza can promote blood circulation and remove blood stasis, promote menstruation and relieve pain, and can introduce drugs into the blood and harmonize various drugs; grape seeds can resist oxidation and protect the cardiovascular system. The combined use of various drugs, scientific compatibility, and synergistic effects can effectively lower blood sugar and reduce complications; and they use less raw materials, are low in price, have a simple preparation process, are easy to store, and are convenient to use.

[0045] The synergist in the present invention has a good synergistic effect with the leaves of Cyclocarya paliurus, and the combination of Pueraria root and Astragalus membranaceus can promote the dissolution of effective ingredients during mixed extraction, and at the same time, the hypoglycemic effect of Coptis chinensis is mutually enhanced, the yin-nourishing effect of Rehmannia root can relieve the dryness and heat that may be caused by other drugs, and Salvia miltiorrhiza plays a unique role in improving blood circulation, and together they provide multi-faceted support for the treatment of hyperglycemia.

[0046] The mixed extraction of the Cyclocarya paliurus leaves and the compatible drugs in the present invention can change the pH value, viscosity and the like during the mixed extraction, thereby improving the dissolution of the effective components in the Cyclocarya paliurus leaves and the extraction efficiency; meanwhile, the spleen-strengthening effect of yam can promote the absorption of the effective components in the Cyclocarya paliurus leaves by the human body, thereby alleviating the disadvantages of spleen and stomach deficiency and coldness that may be caused by burdock roots; meanwhile, the mixed extraction reduces the production links and costs.

[0047] The green sugar provided by the invention has a sweet taste, is not easy to decompose and deteriorate, has low calories, has no side effects, and can replace sugar for use by patients with high blood sugar.

[0048] The present invention is described below by means of specific examples to make the technical solution of the present invention easier to understand and grasp. The experimental methods described in the following examples are conventional methods unless otherwise specified; the reagents and materials can be obtained from commercial channels unless otherwise specified; and different sources have no significant effect on product performance.

[0049] The leaves of Salix palynophora contain a variety of biologically active ingredients, such as flavonoids, triterpenes, etc. These ingredients may play a hypoglycemic role by promoting insulin secretion, enhancing insulin sensitivity, inhibiting gluconeogenesis, etc. However, it cannot replace drugs for treatment, and there are problems such as gastrointestinal discomfort when used. In view of this, the inventors made the present invention through the principle of monarch, minister, assistant and envoy in traditional Chinese medicine and multiple experimental comparisons.

[0050] The following are specific embodiments

[0051] Example 1

[0052] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0053] 10g of Cyclocarya paliurus leaves, 8g of compatible medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 2g of synergist (astragalus, kudzu root, coptis root, rehmannia root and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 7g of grape seeds and 1g of bitter melon.

[0054] The method for preparing green sugar comprises the following steps:

[0055] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0056] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0057] S3: Weighing astragalus, kudzu root, coptis root, rehmannia root and salvia miltiorrhiza according to weight ratio to prepare a synergist;

[0058] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0059] S5: adding grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mixing evenly, and pressing into sheets or blocks.

[0060] Example 2

[0061] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0062] 13g of Cyclocarya paliurus leaf, 8g of compatible medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 2g of synergist (astragalus, kudzu root, coptis root, rehmannia root and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 7g of grape seed and 1g of bitter melon.

[0063] The method for preparing green sugar comprises the following steps:

[0064] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0065] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0066] S3: Weighing astragalus, kudzu root, coptis root, rehmannia root and salvia miltiorrhiza according to weight ratio to prepare a synergist;

[0067] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0068] S5: adding grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mixing evenly, and pressing into sheets or blocks.

[0069] Example 3

[0070] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0071] 17g of Cyclocarya paliurus leaf, 5g of compatible medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 5g of synergist (astragalus, kudzu root, coptis root, rehmannia root and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 5g of grape seed and 2g of bitter melon.

[0072] The method for preparing green sugar comprises the following steps:

[0073] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0074] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0075] S3: Weighing astragalus, kudzu root, coptis root, rehmannia root and salvia miltiorrhiza according to weight ratio to prepare a synergist;

[0076] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0077] S5: adding grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mixing evenly, and pressing into sheets or blocks.

[0078] Example 4

[0079] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0080] 20g of Cyclocarya paliurus leaf, 5g of combined medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 5g of synergist (astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 5g of grape seed and 2g of bitter melon

[0081] The method for preparing green sugar comprises the following steps:

[0082] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0083] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0084] S3: Weigh astragalus, kudzu root, coptis root, rehmannia root, salvia miltiorrhiza and synergist according to weight ratio;

[0085] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0086] S5: adding grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mixing evenly, and pressing into sheets or blocks.

[0087] Comparative Example 1

[0088] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0089] 7g of Cyclocarya paliurus leaf, 10g of combined medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 1g of synergist (astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 10g of grape seed and 0.2g of bitter melon

[0090] The method for preparing green sugar comprises the following steps:

[0091] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0092] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0093] S3: Weigh astragalus, kudzu root, coptis root, rehmannia root, salvia miltiorrhiza and synergist according to weight ratio;

[0094] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0095] S5: Add grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mix well, and press into green sugar in the form of sheets or blocks.

[0096] Comparative Example 2

[0097] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0098] 25g of Cyclocarya paliurus leaf, 3g of combined medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 8g of synergist (astragalus, kudzu root, coptis root, raw rehmannia root and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 2g of grape seed and 4g of bitter melon

[0099] The method for preparing green sugar comprises the following steps:

[0100] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0101] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0102] S3: Weigh astragalus, kudzu root, coptis root, rehmannia root, salvia miltiorrhiza and synergist according to weight ratio;

[0103] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0104] S5: Add grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mix well, and press into green sugar in the form of sheets or blocks.

[0105] Comparative Example 3

[0106] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0107] 25g of Cyclocarya paliurus leaf, 6g of combined medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 5g of synergist (astragalus, kudzu root, coptis root, raw rehmannia root and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 7g of grape seed and 2g of bitter melon

[0108] The method for preparing green sugar comprises the following steps:

[0109] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0110] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0111] S3: Weigh astragalus, kudzu root, coptis root, rehmannia root, salvia miltiorrhiza and synergist according to weight ratio;

[0112] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0113] S5: Add grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mix well, and press into green sugar in the form of sheets or blocks.

[0114] Comparative Example 4

[0115] A green sugar capable of effectively treating hyperglycemia, the raw materials comprising the following components:

[0116] 25g of Cyclocarya paliurus leaf, 6g of combined medicine (wolfberry, burdock root, yam and ginseng in a weight ratio of 8:11:10:6), 5g of synergist (astragalus, kudzu root, coptis root, raw rehmannia root and salvia miltiorrhiza in a weight ratio of 6:6:1:4:4), 7g of grape seed and 2g of bitter melon

[0117] The method for preparing green sugar comprises the following steps:

[0118] S1: weighing grape seeds according to a weight ratio to prepare grape seed extract;

[0119] S2: Weighing bitter melon according to weight ratio to prepare bitter melon extract;

[0120] S3: Weigh astragalus, kudzu root, coptis root, rehmannia root, salvia miltiorrhiza and synergist according to weight ratio;

[0121] S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract;

[0122] S5: Add grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mix well, and press into green sugar in the form of sheets or blocks.

[0123] Experiment on the effect of hyperglycemia treatment:

[0124] (1) Experimental animals: SD rats aged 8-12 weeks, weighing 200±20 g.

[0125] (2) Test material configuration:

[0126] High-sugar and high-fat feed formula: 55% rat maintenance feed + 20% sucrose + 17% lard + 1.5% cholesterol + 6.5% egg yolk powder.

[0127] Preparation of 2% streptozotocin (STZ) solution: Dissolve STZ in sodium citrate buffer with a pH value between 4.2 and 4.5 to prepare a 2% streptozotocin solution.

[0128] (3) Modeling grouping: Rats were fed with practical maintenance feed for one week under the conditions of an ambient temperature of 22 (±2)°C, a relative humidity of 50%-60%, and a light cycle of 12 hours light / 12 hours dark, and then the following operations were performed:

[0129] Rats were fed with a high-sugar and high-fat diet for 4 weeks, fasted for 12 hours, and injected with 2% streptozotocin solution at 35 mg / kg into the tail vein to prepare a rat hyperglycemia model; after continuing to be fed with a high-sugar and high-fat diet for 1 week after injection, blood was collected between the tails to measure fasting blood sugar, and those with blood sugar >10mmol / L were considered to have successfully established the model.

[0130] Ten rats with successful modeling were randomly selected as one group, namely, Example 1-4 group, model group, and positive drug control group, with 10 rats in each group.

[0131] Ten unmodeled rats were fed with maintenance diet for 4 weeks at an ambient temperature of 22 (± 2)°C, a relative humidity of 50%-60%, and a photoperiod of 12 hours light / 12 hours dark, as a normal control group.

[0132] (4) Drug intervention treatment: The Example 1-4 group and the Comparative Example 1-4 group were given the green sugar prepared in the respective examples, with an oral administration dose of 100 mg / kg; the positive drug group was given 100 mg / kg of metformin by oral administration; the model group and the normal control group were given 200 mg / kg of normal saline, respectively, and the drug intervention lasted for 2 weeks; during the intervention period, the model group, the positive drug group, the Example 1-4 group and the Comparative Example 1-4 group continued to be fed with a high-sugar and high-fat feed, and the normal control group continued to be fed with a maintenance feed.

[0133] (5) The rats were fasted for 12 hours on the day before the drug intervention period and on the last day of the intervention period, and blood was collected from the tail. The fasting blood glucose levels were measured using a blood glucose analyzer. The changes in blood glucose levels of the rats in each group before and after drug intervention are shown in Table 1 below:

[0134]

[0135]

[0136] Table 1

[0137] Through the test results in Table 1, combined with Example 1-4 and Comparative Example 1-2 and the positive drug group, we can see that when the constituent raw materials are counted by weight, the green sugar prepared by the present invention has a good therapeutic effect on hyperglycemia, with 10-20 parts of Cyclocarya paliurus leaves, 5-8 parts of the compatibility drug, 2-5 parts of the synergist, 5-7 parts of grape seeds and 1-2 parts of bitter melon.

[0138] At the same time, the poor therapeutic effect of Comparative Examples 3 and 4 is due to the excessive proportion of Salix palynoides in the green sugar, which destroys the balance of the medicine and worsens the efficacy. The inventors reasonably speculate that the efficacy can be further improved by changing the weight ratio of each medicinal material in the combination of medicines. Therefore, in order to verify the weight ratio of the combination of medicines, the inventors selected Example 3 as the basis, and made Example 5-8 and Comparative Examples 3-4 by adjusting the weight ratio of each medicinal material in the combination of medicines.

[0139] Six groups of rats were selected after successful modeling and given the green sugar prepared according to the weight ratio of the respective combinations of drugs in the embodiment and the comparative example, with an oral administration dose of 100 mg / kg. The drug intervention lasted for 2 weeks. The rats were fasted but not watered for 12 hours on the day before the drug intervention period and on the last day of the intervention period, and blood was collected from the tail. The fasting blood glucose level was measured with a blood glucose analyzer, and the test results were recorded as shown in Table 2 below.

[0140]

[0141]

[0142] Table 2

[0143] Through the test results in Table 2, combined with the data of Example 5-8 and Comparative Example 5-6, it can be seen that when wolfberry, burdock root, yam and ginseng are used in combination, and the weight ratio of wolfberry, burdock root, yam and ginseng is (7-9): (10-12): (8-12): (5-8), the green sugar obtained has a better blood sugar lowering effect. The reason is analyzed as follows: the burdock root in the weight ratio can clear away heat and dampness, purge fire and detoxify, and has the effect of lowering blood sugar, and together with the Cyclocarya paliurus leaves, it strengthens the effect of clearing heat and lowering blood sugar; the yam in the weight ratio can strengthen the spleen, promote the body's absorption of the effective ingredients in the Cyclocarya paliurus leaves, and alleviate the disadvantages of spleen and stomach deficiency and cold that may be caused by the burdock root; the ginseng in the weight ratio can nourish the spleen and lungs, promote the production of fluid and quench thirst, promote the body's absorption and utilization of nutrients, and is also effective for polydipsia caused by hyperglycemia; the wolfberry in the weight ratio can promote the production of fluid and quench thirst, and nourish the liver and kidneys; the combined drugs in the weight ratio can act synergistically to provide multi-faceted support for the treatment of hyperglycemia, and the mixed extraction of the Cyclocarya paliurus leaves and the combined drugs can change the pH, viscosity, etc. during the mixed extraction, improve the dissolution of the effective ingredients in the medicinal materials, and improve the extraction rate of the effective ingredients.

[0144] Similarly, the inventors also speculated that the weight ratio of the drugs in the synergist would also affect the efficacy of the green sugar obtained. Based on the Example 8 group, the Example 8-11 groups and the Comparative Examples 7-8 groups were prepared by adjusting the weight ratio of each medicinal material in the synergist.

[0145] Six groups of rats with successful modeling were given the green sugar prepared according to the weight ratio of the respective combinations of drugs in the embodiment and the comparative example, with an oral administration dose of 100 mg / kg. The drug intervention lasted for 2 weeks. The rats were fasted but not watered for 12 hours on the day before the drug intervention period and on the last day of the intervention period, and blood was collected from the tail to measure their fasting blood glucose levels using a blood glucose analyzer.

[0146] Record the test results, see Table 3 below.

[0147]

[0148] Table 3

[0149] According to the test results of Table 3, combined with the data of Example 9-11 and Comparative Example 7-8, when the synergist contains astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza, the weight ratio of astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza is (6-8): (6-8): (1-2): (4-6): (4-6), the green sugar obtained has a good hypoglycemic effect. The reason for the analysis is that when the weight ratio of astragalus, kudzu root, coptis root, raw rehmannia and salvia miltiorrhiza is mixed and extracted, the combination of kudzu root and astragalus can promote the dissolution of effective ingredients, and at the same time, it can enhance the hypoglycemic effect of coptis root. The nourishing effect of rehmannia can relieve the dryness and heat that other drugs may bring, and salvia miltiorrhiza plays a unique role in improving blood circulation. As a synergist, they provide multi-faceted support for the treatment of hyperglycemia.

[0150] Clinical trial results:

[0151] In the spirit of being responsible to patients, clinical trials only test green sugar that has shown therapeutic effects in rat hyperglycemia tests.

[0152] 120 high-glycemic volunteers with prediabetes (fasting blood sugar value between 6.1mmol / L-7.0mmol / L; blood sugar between 7.8mmol / L-11.1mmol / L two hours after a meal) without drug intervention were selected, half of them were male and half were female. The volunteers had symptoms of high blood sugar for more than 3 months and were between 45 and 55 years old. The volunteers were randomly divided into 12 groups, 10 people in each group (half male and half female), and their original living, eating and working habits were not changed. Among them, 11 groups took 1 dose of green sugar prepared by Examples 1-11 of the present invention every day, and the remaining group took metformin as a control group. They took it for 1 month, and measured blood sugar before and after treatment. The results are shown in Table 4:

[0153]

[0154]

[0155] Table 4

[0156] It can be seen from the above data that the green sugar provided by the present invention has a good therapeutic effect on hyperglycemia, and clinical observations also show that the green sugar prepared by the present invention has a good therapeutic effect on polydipsia, polyuria, blurred vision, and numbness of hands and feet caused by hyperglycemia, has no other adverse reactions, and can be taken for a long time.

[0157] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent changes made using the specification of the present invention are also included in the patent protection scope of the present invention.

Claims

1. A green sugar suitable for patients with hyperglycemia, characterized in that: According to the weight ratio, the raw materials are composed of the following components: The content of Cyclocarya paliurus leaves is 10-20 parts, the content of the compatibility drug is 5-8 parts, the content of the synergist is 2-5 parts, the content of the grape seeds is 5-7 parts, and the content of the bitter melon is 1-2 parts.

2. The green sugar according to claim 1, characterized in that The combination of medicines includes wolfberry, burdock root, yam and ginseng, and the weight ratio of wolfberry, burdock root, yam and ginseng is (7-9): (10-12): (8-12): (5-8).

3. The green sugar according to claim 1, characterized in that The synergist comprises astragalus, kudzu root, coptis root, raw rehmannia root and salvia miltiorrhiza, and the weight ratio of astragalus, kudzu root, coptis root, raw rehmannia root and salvia miltiorrhiza is (6-8): (6-8): (1-2): (4-6): (4-6).

4. A method for preparing the green sugar as claimed in any one of claims 2 to 3, characterized in that: The following steps are involved: S1: weighing grape seeds according to a weight ratio to prepare grape seed extract; S2: Weigh bitter melon according to weight ratio to prepare bitter melon extract; S3: Weighing astragalus, kudzu root, coptis root, raw rehmannia root and ligusticum chuanxiong according to weight ratio to prepare a synergist; S4: Weigh the Cyclocarya paliurus leaves and the drugs according to the weight ratio to prepare a mixed extract; S5: adding grape seed extract, bitter melon extract and enhancer to the mixed extract in sequence, mixing evenly, and pressing into tablets or blocks.

5. The method for preparing green sugar according to claim 4, characterized in that: The preparation method of the grape seed extract in step S1 is as follows: S11: Weigh high-quality grape seeds according to weight ratio; S12: placing grape seeds into an extraction kettle; S13: Introduce carbon dioxide gas into the equipment, adjust the pressure to 10-40Mpa, and the temperature to 30-60°C; S14: stirring the carbon dioxide and grape seeds in the reaction kettle to allow them to fully contact and extract for 1-3 hours; S15: the extracted extract enters a separation kettle along with the supercritical fluid, and the pressure and temperature are changed to convert the carbon dioxide into gas, thereby separating the grape seed extract; S16: opening the separation kettle to collect the grape seed extract; S17: Analyze the extract using high performance liquid chromatography to ensure that the extract meets the quality standards; S18: Drying and collecting the grape seed extract.

6. The method for preparing green sugar according to claim 4, characterized in that: The preparation method of the bitter melon extract in step S2 is as follows: S21: Weigh fresh bitter melon by weight, wash and slice; S22: taking a 50-70% ethanol aqueous solution and placing it in an extraction container, and adding bitter melon slices, wherein the liquid-to-material ratio of the ethanol aqueous solution to the bitter melon slices is 5:1-20:1; S23: The temperature is controlled at 40-80°C and the container is placed in an ultrasonic environment for 0.5-2 hours; S24: centrifugation and filtration to separate the filtrate from the residue; S25: The filtrate is subjected to chromatographic separation and purification, and concentrated to obtain the bitter melon extract.

7. The method for preparing green sugar according to claim 4, characterized in that: The preparation method of the synergist in step S3 is as follows: S31: Weigh astragalus, kudzu root, coptis root, raw rehmannia root, salvia miltiorrhiza according to weight ratio, wash, dry, mix and grind; S32: adding phosphate buffer to a container, adding crushed medicinal materials, wherein the ratio of phosphate buffer to medicinal materials is 5:1-10:1, stirring to prepare a medicinal material suspension; S33: controlling the temperature at 40-60° C., adding cellulase, wherein the weight ratio of the cellulase to the medicinal material is 0.5-2%, stirring, and performing enzymolysis for 1-3 hours; S34: reflux extraction of the enzymatically hydrolyzed medicinal material using 60-80% ethanol; S35: filtering the medicinal residue to obtain an extract, S36: spray-drying the extract to make powder to obtain the extract 1.

8. The method for preparing green sugar according to claim 4, characterized in that: The preparation method of the mixed extract in step S4 is as follows: S41: Weigh the leaves of Cyclocarya paliurus by weight, weigh the drugs to be used in combination by weight, wash, dry, mix and grind; S42: adding water to a container, adding crushed medicinal materials, with a water to medicinal material ratio of 5:1-10:1, and soaking for 1-2 hours; S43: After boiling, control the temperature to 80-100° C., stir, and extract for 1-2 hours; S44: filtering the medicinal residue to obtain an extract; S46: spray-drying the extract to make powder to obtain the mixed extract.

9. The method for preparing green sugar according to claim 3, characterized in that: The flake or block green sugar prepared in step S5 is stored in a cool and dry environment.

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