Homotypic cysteine measuring method and its reagent

Abstract

The invention relates to an enzymology measuring method and reagent with circular increment technology, applied to measure the content of homeotypic cysteine in the measured sample. The character is: with a circular increment technology, increases the measurement sensitivity, thus it can carries on automatic measurement to the homeotypic cysteine in the sample liquid like normal enzymology diagnosing agent. The homeotypic cysteine in the sample liquid reacts with the S-adenosine-L-methionine repeatedly and circularly under the effect of homeotypic cysteine methyl kinase and adenosine homeotypic cysteine, generates the adenosine, the speed of the adenosine generation is in direct proportion to the homeotypic cysteine in the sample, through the generation speed, the aim to measure the content of the homeotypic cysteine in sample can be achieved.

Description

Technical field: [0001] The invention relates to a method for determining homocysteine ​​and its reagents Background technique: [0002] Homocysteine ​​(Homocysteine) is a sulfur-containing amino acid that is produced in cells by demethylation of methionine. Recent studies have shown that homocysteine ​​produces superoxide and peroxide damages vascular endothelial cells, changes the function of coagulation factors, increases the tendency of thrombosis, promotes atherosclerosis and thrombosis, and increases the incidence of cardiovascular diseases and Mortality increases, so measuring the concentration of homocysteine ​​in blood is of great clinical significance. When homocysteine ​​accumulates in cells and enters the blood circulation, most of it exists in the oxidized form and binds to proteins, while the reduced form of homocysteine ​​accounts for only 1% in the blood. To determine the content of total homocysteine ​​in blood, it is generally necessary to use a reducing ...

AI Technical Summary

Problems solved by technology

The common features of the above methods are that special measuring instruments are required, the operation is complicated, the test takes a long time, it cannot be applied to the automatic analysis of clinical large flow, and it is expensive

[0004] Due to the low content of homocysteine ​​in the sample, conventional clinical enzymology assay methods cannot achieve the required assay sensitivity, which limits the adoption of conventional methods

In terms of technology for the determination of homocysteine ​​by enzymatic methods, Naoto Matsuyama et al. (see US Patent #6686172) invented an acyclic method using homocysteine ​​methyltransferase and adenosylhomocysteinase Enzyme determination technology, a method to improve the sensitivity of the determination, but the method is cumbersome to operate, the reagents are complicated, and the sulfhydryl compound needs to be added before the determination, and multiple blank determinations must be carried out at the same time during the determination, which increases the inaccuracy of the determination method

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