Technology for cultivating triploid high-mogroside content momordica grosvenoris

Abstract

The invention discloses a technology for cultivating triploid momordica grosvenoris which is high in mogroside content and whole momordiaca grosvenori utilization rate. The technology comprises the steps of selecting field fresh momordica grosvenoris with good appearances, disinfecting the whole momordica grosvenoris, inducing young embryos to bud and preparing the budded embryos into virus-free seedlings; performing polyploid induction and cell flow analysis on female plants analyzed and identified from the virus-free seedlings through restricted fragment length polymorphism (RFLP); hybridizing stable tetraploid female plants with diploid male plants with good commodity natures; and harvesting fruits in autumn and sowing seeds in the fruits in a nutrition pot, planting virus-free seedlings which are analyzed to be triploid female plants through a flow cytometry and the RFLP, and hybridizing virus-free seedlings with diploid male plants with good commodity natures to obtain the triploid high-mogroside content momordica grosvenoris, namely, the born fruits.

Description

technical field [0001] The invention combines plant tissue culture, polyploid induction and genetic breeding hybridization techniques to cultivate and obtain triploid high sweet glycoside Luo Han Guo, which belongs to the field of modern biotechnology. Background technique [0002] The fruit of Luo Han Guo is cool and sweet, and has the effects of clearing away heat and moistening the lungs, cooling the blood, moistening the intestines and laxative, especially the sweetness of mogroside V is about 300 times that of sucrose. Food additive, which integrates natural sweeteners and health products, is internationally recognized as the natural sweetener with the best comprehensive properties. Luo Han Guo is considered to be a very potential sweet plant variety. For a long time, due to the backward cultivation technology of Luo Han Guo, serious virus diseases and degraded varieties, the resources of Luo Han Guo are scarce. In the past ten years, through the detoxification techno...

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Problems solved by technology

[0005] The inventor found through long-term research that the above-mentioned technique has the instability problem of tetraploids and triploids obtained, (can you further explain its unstable performance), and in its technical process, it is treated with colchicine, Combined with the induction of clustered buds, the cycle of obtaining tetraploids is long, the chimerism rate is high, and the repeatability is poor, which affects the popularization and application of this technology

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