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Method for inducing polyploid through wild jujube adventitious buds

A technology of polyploid and sour jujube, applied in the biological field, can solve the problem of difficult to obtain homozygous tetraploid, and achieve the effect of overcoming chimerism and high efficiency

Inactive Publication Date: 2014-10-08
BEIJING FORESTRY UNIVERSITY
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  • Claims
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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide the optimal treatment period, suitable concentration and treatment time of colchicine in polyploids induced by the adventitious bud approach of jujube, so as to solve the problem that it is difficult to obtain homozygous tetraploids by existing methods

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  • Method for inducing polyploid through wild jujube adventitious buds
  • Method for inducing polyploid through wild jujube adventitious buds
  • Method for inducing polyploid through wild jujube adventitious buds

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Embodiment Construction

[0030] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail with reference to the drawings and specific embodiments.

[0031] The embodiment of the present invention aims at the problem that it is difficult to obtain homozygous tetraploids in the existing methods, and provides a method for inducing polyploids through the adventitious buds of jujube jujube, comprising the following steps:

[0032] Initiate culture, cut the young leaves of jujube jujube aseptic seedlings, inoculate them on the adventitious bud initiation medium 1 after scratching and carry out 9~11d pre-(dark) cultivation;

[0033] For induction culture, transfer the leaves to a liquid medium containing 40-80mg / L colchicine for polyploid induction culture, culture in a shaker at 100rmp in the dark for 2-3 days, and then transfer to the adventitious bud initiation medium. Continue to culture in dark for 8-1...

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Abstract

The invention provides a method for inducing polyploid through wild jujube adventitious buds, relates to the biotechnical field, and the problem that homozygous tetraploid is hard to obtain through existing methods is solved. The method provided by the invention comprises the following steps: starting of cultivation, induction culture, elongating cultivation, strong seedling cultivation and detection on young leaves. The method can be used for cultivating wild jujube homozygous polyploid so as to support the genetic improvement of jujube trees.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for inducing polyploidy through adventitious buds of jujube. Background technique [0002] Jujube (Zizyphus.jujuba Mill.) is a plant of the genus Zizyphus.Mill. in the family Rhamnaceae. It is a characteristic and advantageous economic tree species native to China. A variety of amino acids and vitamins, as well as medicinal ingredients such as polysaccharides, trace elements and the anticancer substance cyclic adenosine monophosphate, are dual-purpose tonics for medicine and food. [0003] Wild jujube (Ziziphus spinosus Hu.) is native to North China. It is wild, drought-resistant and barren, and has played an important role in the development of mountainous areas. The wild jujube has a long flowering period and is a good nectar source plant. Its seeds are plump and can be used as medicine. As a food, wild jujube has high nutritional value and contains various trace element...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 李颖岳王岩庞晓明王欢康亚璇
Owner BEIJING FORESTRY UNIVERSITY
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