Method for inducing phalaenopsis protocorm to generate polyploid by colchicine

A colchicine and protocorm technology, applied in the field of polyploid breeding, can solve the problems of low induction rate and low protocorm survival rate

Active Publication Date: 2021-02-02
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI +1
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Problems solved by technology

[0004] The difficulty of solving the above problems and defects is: the concentration and treatment time of the colchicine co-cultivation method to

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  • Method for inducing phalaenopsis protocorm to generate polyploid by colchicine
  • Method for inducing phalaenopsis protocorm to generate polyploid by colchicine
  • Method for inducing phalaenopsis protocorm to generate polyploid by colchicine

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[0051] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0052] Aiming at the problems existing in the prior art, the present invention provides a method for inducing polyploidy of Phalaenopsis protocorms with colchicine. The present invention will be described in detail below in conjunction with the accompanying drawings.

[0053] like figure 1 As shown, the colchicine-induced polyploidy method of the protocorm of Phalaenopsis provided by the embodiments of the present invention comprises the following steps:

[0054] S101, polyploid induction: transfer protocorms to 1 / 2MS+1mg / l6-BA+0.2mg / l NAA+5g / l agar+20g / l sucrose solid medium containing colchicine for induction treatment ...

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Abstract

The invention belongs to the technical field of polyploid breeding, and discloses a method for inducing phalaenopsis protocorm to generate polyploid by colchicine. The method comprises the following steps of transferring the protocorm into a 1/2MS+1mg/l 6-BA + 0.2 mg/l NAA+5g/l agar+20g/l sucrose solid culture medium containing 0.05% of colchicine, and culturing for 15 days for induction treatment; washing the protocorm for 3-4 times by using sterile water after treatment, absorbing moisture by using filter paper, transferring the protocorm into a 1/2MS+5g/l agar+20g/l sucrose culture medium,and carrying out subculture for 2-3 times; performing polyploid identification, namely respectively carrying out morphological observation, stomatal identification and ploidy identification; and performing data statistics. According to the method, colchicines with different concentrations and different treatment time are used for doubling the phalaenopsis protocorm by a co-culture method, so thata foundation is laid for polyploid induction culture of a new phalaenopsis variety with large flower size, bright color and high ornamental value.

Description

technical field [0001] The invention belongs to the technical field of polyploid breeding, in particular to a method for inducing colchicine to produce polyploids in moth orchid protocorms. Background technique [0002] At present, Phalaenopsis is a plant of the genus Phalaenopsis in the family Orchidaceae. It has various colors and looks like a butterfly. It has the reputation of "Queen of Orchids". In the flower market, Phalaenopsis is popular in the flower market for its large and colorful flowers. The cultivated species are mainly tetraploid, triploid and aneuploid, of which tetraploid accounts for the vast majority, up to 82%. And polyploidy often has the advantages of strong plant type, huge leaf and flower organs, strong stress resistance, etc. Therefore, polyploid breeding induction for Phalaenopsis is an indispensable way for Phalaenopsis breeding. The tissue culture technology of orchids is relatively mature, so most orchids use tissue culture combined with doubli...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 葛红吴婷贾瑞冬杨树华朱剑非赵鑫李秋香
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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