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Acipenser dabryanus microsatellite marker as well as screening method and application of acipenser dabryanus microsatellite molecular marker

A Dabry's sturgeon and microsatellite technology, applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., to achieve the effect of low cost

Inactive Publication Date: 2017-02-22
FISHERIES INST SICHUAN ACADEMY OF AGRI SCI
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Problems solved by technology

Although SSR markers have good effect and high reliability, the key point lies in the development of SSR primers

Method used

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  • Acipenser dabryanus microsatellite marker as well as screening method and application of acipenser dabryanus microsatellite molecular marker

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Embodiment Construction

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0020] Acquisition and verification of microsatellite loci and specific primers in Acipenser dabryi:

[0021] Dabry's sturgeon sample collection and RNA extraction:

[0022] Four healthy Dabry's sturgeons with differentiated gonads were selected from Sichuan Fisheries Research Institute, and their gonads were dissected to extract total RNA. In a bowl, use liquid to quickly grind into powder, put it into a 1.5mL centrifuge tube, and add 1mL TRIzol; (2) Add 200μL of chloroform to t...

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Abstract

The invention belongs to the field of microsatellite molecular markers and particularly relates to an acipenser dabryanus microsatellite marker as well as a screening method and application of the microsatellite molecular marker, wherein the acipenser dabryanus microsatellite marker has the nucleotide sequence of any one or more of SEQ ID No.1-8. In the invention, the development of microsatellite molecular markers using the high-throughput sequencing technology is faster and costs less than a traditional method; and moreover, the functional traits of corresponding EST sequence can be found while the microsatellite sites are obtained, and the gene relevance is relatively strong. In the invention, through the developed microsatellite sites, basic data is provided for the genetic diversity of acipenser dabryanus, and the study fields such as genetic diversity analysis, genetic map establishment, gene location, variety identification, germplasm conservation, quantitative character gene analysis and parent-child relationship identification can be carried out. According to actual needs, any one or more markers disclosed by the invention can be adopted, or any one or more pairs of specific primers corresponding to related markers can be analyzed and studied.

Description

technical field [0001] The invention belongs to the field of microsatellite molecular markers, in particular to a dabrye sturgeon microsatellite marker and its screening method and application. Background technique [0002] Microsatellite markers (SSRs: simple sequence repeats) or short tandem repeats (Short tandem repeats, STRS) are DNA molecular markers widely used in the field of genetics. SSRs are simple repeating DNA segments that widely exist in eukaryotic genomes, and generally each repeating unit is 1-6 bases. Compared with other genetic markers, SSR has the advantages of numerous loci, high degree of polymorphism, low requirement for DNA template during detection, simple and rapid detection, and is a good genetic marker. The high mutation, co-dominant expression and ubiquity of microsatellite DNA in eukaryotic genomes make it a superior molecular marker for the analysis of population genetic diversity, identification of kinship, genome mapping and genetic breeding ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 陈叶雨刘亚杜军龚全赵刚
Owner FISHERIES INST SICHUAN ACADEMY OF AGRI SCI
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