Method for establishing SSR (Simple Sequence Repeat) fingerprint spectrum of broad beans

Abstract

The invention provides a method for establishing an SSR (Simple Sequence Repeat) fingerprint spectrum of broad beans, and belongs to the technical field of plant variety identification and breeding. The method provided by the invention provides a marker combination consisting of 21 pairs of broad bean genome SSR primers, and the nucleotide sequences of which are shown in SEQ ID NO. 1 to 42 respectively; the broad bean varieties are identified by PCR (Polymerase Chain Reaction) amplification technology, non-denaturing polyacrylamide gel electrophoresis or capillary electrophoresis detection technology, and the identification of broad bean seed varieties and genetic diversity assessment work can be completed in a short time. The method has the advantages of time saving, high efficiency, fastness, accuracy, and convenience in operation, and the identification results are not easily affected by the environment. The method provided by the invention has the advantages of effectively monitoring the authenticity of the broad bean seeds, revealing the genetic variation and genetic relationship of the varieties from the DNA level, protecting the crop varieties, preventing the fake and inferior varieties from entering the market, and also providing a technical support for the reasonable utilization of excellent germplasm during the breeding of broad bean varieties, and has good application prospects.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and plant variety identification, in particular to a method for constructing broad bean SSR fingerprints and a method and application for identifying broad bean varieties using the constructed fingerprints of different varieties of broad beans. Background technique [0002] Broad bean (Vicia faba L.), as a grain, vegetable, feed, and green manure crop, occupies an important position in my country's crops. Broad beans are produced and distributed in all continents of the world, and more than 43 countries around the world produce broad beans. Broad beans have been cultivated in my country for more than 2,000 years. Except for Shandong, Hainan and the three northeast provinces, which are rarely planted, broad beans are planted in other parts of the country. In the past 10 years, the planting area of ​​broad beans in China has reached more than 1.4 million hectares 2 , accounting for abou...

AI Technical Summary

Problems solved by technology

This method is simple and economical, but because many morphological traits have a long identification period and are easily affected by the environment, it cannot accurately and quickly provide identification basis for new variety infringement cases, and there are often deviations in the understanding of certain traits by different testers , leading to the problem of inconsistent judgment results

With the centralized use of breeding parents, the traditional morphological and cytological identification methods have many traits and a large workload. It is difficult to accurately and truly identify the differences in broad bean varieties. Therefore, analysis and identification at the molecular level are required.

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