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Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations and application thereof

A whole genome and sequence information technology, applied in the biological field, can solve problems such as heavy workload, error-prone, and difficult sequence comparison, and achieve the effects of easy statistics, high polymorphism, and good repeatability

Active Publication Date: 2011-10-19
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, most plant genome sequencing projects have not been completed, and it is difficult to perform sequence comparison. The ideal test results can only be obtained by repeatedly screening core primer combinations, which is a huge workload and error-prone

Method used

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  • Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations and application thereof
  • Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations and application thereof
  • Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Research on Genetic Diversity of Watermelon Breeding Materials

[0034] 1. Materials and methods:

[0035] 1.1 Materials

[0036] The test materials were 17 resequencing materials and 100 watermelon breeding materials.

[0037]The 17 watermelon resequencing materials covered two watermelon species, Citrullus lanatus and Citrullus colocynthis, and two varieties, Citrullus lanatus var.lanatus and Citrullus lanatus var.citroides. Rich in types, from seedless to seeded, from yellow flesh to red flesh, from small melons to large melons, basically covering the main ecological types and main agronomic traits of watermelon, reflecting the diversity of germplasm as much as possible, with a high Genetic diversity (Table 1).

[0038] 100 watermelon breeding materials are breeding materials with clear genetic relationship, which are mainly used to adjust and verify core primers.

[0039] 1.2 Research Methods

[0040] 1.2.1 Genomic DNA extraction

[0041] 1ml / tube...

Embodiment 2

[0066] Embodiment 2 Validity test of the SSR core primer set of the present invention

[0067] Among the 100 breeding materials, there are many materials with close genetic relationship, such as Zhongzi Jingmu, K209, Zhongzhong Jingmu are all bred from the same excellent single plant system, 159 Baipi is the male parent of She 948-1 Sister line, Jingxin No. 1 F 1 , Jingxin No. 2 F 1 , Jingxin No. 7 F 1 have similar ancestry. Cluster analysis was performed using 23 core primer combinations, and the results showed that the genetic similarity coefficients of the three groups of materials were all greater than 0.9. It shows that the difference of DNA level among materials reflected by 23 core primers is basically consistent with the results of pedigree analysis of material sources.

[0068] At the same time, the cluster analysis results of 100 breeding materials showed that the genetic similarity coefficient between She 948 female parent-1 and She 948 female parent-2 was 1. A...

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Abstract

Watermelon complete genomic sequence information based analyzed and developed SSR core primer combinations. The primer combinations comprise primers, of which nucleotide sequences as shown in the sequence table SEQ ID No.1-46. The obtained SSR core primer combinations in the invention can reflect the genetic diversity of watermelon germplasm resources to the maximum, has advantages of high polymorphism, good repeatability, reliable and stable marking, and are convenient for statistics. The set of primers provided by the invention are utilized to carry out a genetic diversity analysis, so as to accurately reflect the genetic relationship of tested materials to the maximum.

Description

technical field [0001] The invention relates to a core primer sequence, specifically a set of SSR core primer sequences developed based on the watermelon genome sequence and an application thereof, belonging to the field of biotechnology. Background technique [0002] Watermelon, as an important economic crop, plays an important role in the adjustment of planting industry structure in my country and in promoting farmers to become rich and increase their income. At present, the problem of seed quality in watermelon production is relatively prominent and common. Insufficient purity of seeds and incidents of fake seeds pitting farmers often occur. The phenomenon of the same name with different objects and different objects with the same name is very common, which seriously interferes with the healthy development of the seed industry. It is also a threat to variety innovation and knowledge. Serious damage to the protection of property rights. Conventional variety approval / ident...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 许勇张海英王慧宫国义郭绍贵任毅张岩
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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