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Compound successive crop-resistance micro-ecological formulation special for cotton and special bacterial strain and use thereof

A micro-ecological preparation and anti-heavy cropping technology, which is applied in the field of cotton-specific compound anti-heavy-cropping micro-ecological preparations, can solve the problems affecting the sustainable development of cotton production and the rapid spread of diseased seeds, and achieve a significant effect of increasing production and reducing pollution and its harmful effects. Residue of substances, effect of reduction in usage

Inactive Publication Date: 2009-01-28
北京康泰正茂农业科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the promotion of hybrid cotton or insect-resistant hybrid cotton, agricultural extension units have relaxed their ideological vigilance, ignored the quarantine system for cotton "two wilts", and frequently transferred seeds (especially in disease-endemic areas), resulting in the rapid development of diseased seeds. Spread, especially in the old cotton fields of long-term continuous cropping, the "two wilts" have become the main obstacle to the high and stable cotton yield, seriously affecting the sustainable development of cotton production

Method used

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  • Compound successive crop-resistance micro-ecological formulation special for cotton and special bacterial strain and use thereof
  • Compound successive crop-resistance micro-ecological formulation special for cotton and special bacterial strain and use thereof
  • Compound successive crop-resistance micro-ecological formulation special for cotton and special bacterial strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1, the preparation of compound anti-cropping micro-ecological preparation

[0011] 1. Acquisition of strains for controlling cotton cropping disease

[0012] Two high-efficiency strains, Trichoderma viride (Trichoderma viride) KTF001 and Bacillus subtilis (Bacillus subtilis) KTB001, were isolated and screened from healthy plants and roots of cotton cropping disease field.

[0013] The isolation and screening methods of Trichoderma viride (Trichoderma viride) KTF001 and Bacillus subtilis (Bacillus subtilis) KTB001 are as follows:

[0014] (1) Separation

[0015] Trichoderma viride isolation plate medium: 200g potato, 20g glucose, 20g agar, 1000ml water, 1g sodium propionate. Then pack it into Kelvin bottles or test tubes, place in a sterilizer, sterilize at 0.11MPa pressure, 121°C for 30min, cool down and make a slope for use.

[0016] Bacillus subtilis isolation plate medium (beef extract peptone agar medium): beef extract 0.3g / 100ml, peptone 0.5g / 100ml, N...

Embodiment 2

[0067] Embodiment 2, preparation of compound anti-cropping probiotics

[0068] The preparation method of the composite anti-cropping micro-ecological preparation is the same as in Example 1, except that the solid preparation of Trichoderma viride (Trichoderma viride) KTF001 CGMCC No.2562 and Bacillus subtilis KTB001 CGMCC No.2564 are mixed in a mass ratio of 10:3 , to prepare the compound anti-double-cropping microecological preparation for preventing and controlling cotton blight, Verticillium wilt, blight, damping-off and other diseases, and each gram of compound anti-double-cropping microecological preparation contains Trichoderma viride (Trichoderma viride) KTF001 CGMCC No.2562 The content of chlamydospores is 5 billion cfu / g, and the content of spores of Bacillus subtilis KTB001 CGMCC No.2564 is 15 billion cfu / g.

Embodiment 3

[0069]The cotton field test effect of the compound anti-heavy cropping micro-ecological preparation of embodiment 3, embodiment 1

[0070] The experimental site with a history of planting cotton for 5 consecutive years was selected. The incidence of blight, fusarium wilt and verticillium wilt were severe over the years, and blight blight, fusarium wilt and verticillium wilt occurred naturally during the experiment. Select the terrain to be flat, and the soil properties are consistent, and the uniform middle plot of fertility is planted with No. 36 cotton Zhongmian Institute (Xinjiang Jinmian Seed Industry Co., Ltd.) to establish 2 treatments, and 1 is to spray the method of embodiment 1 at the seedling stage and flowering stage respectively. The compound anti-heavy cropping micro-ecological preparations were used once each, 4 kg per mu; treatment 2 was spraying water at the seedling stage and flowering stage respectively as a control; each treatment was repeated 4 times, with a...

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Abstract

The invention discloses a composite anti-continuous cropping microbial ecological agent specially used in cotton and the application thereof. The anti-continuous cropping microbial ecological agent of the invention takes bacillus subtilis KTB001 CGMCC No.2564 and trichoderma viride KTF001 CGMCC No.2562 as active ingredients, wherein, the content of the gemma of a fungus of the bacillus subtilis KTB001 CGMCC No.2564 is 50 hundred million cfu / g preparation; the content of chlamydospore of trichoderma viride KTF001 CGMCC No.2562 is 5 hundred million cfu / g preparation. The composite anti-continuous cropping microbial ecological agent of the invention can prevent and control cotton seedling diseases and verticillium wilt. Furthermore, the agent has the effectiveness of increasing cotton output, increasing cotton quality and enhancing cotton stress resistance, and the like, is beneficial to improving soil and reducing environmental pollution, and provides safeguard for producing cotton with excellent quality.

Description

technical field [0001] The invention relates to a special compound anti-repeat cropping micro-ecological preparation for cotton, special bacterial strains and applications thereof. Background technique [0002] Cotton is the main raw material for people's clothing, the textile industry and an important product for exporting foreign exchange. It is of great significance to the international people's livelihood. However, in recent years, due to the adjustment of the agricultural planting structure, the phenomenon of double-cropping planting is very common, which makes cotton double-cropping diseases common, such as cotton blight, verticillium wilt, blight, damping-off and other seedling diseases, especially cotton fusarium wilt and yellow Wilt is a serious hazard. [0003] Cotton Fusarium wilt and Verticillium wilt (referred to as "two wilts") is one of the plant quarantine diseases. It is highly pathogenic among cotton diseases and causes heavy losses. It is also an importa...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00C12N1/20C12N1/14C12R1/125C12R1/885
CPCY02A40/10
Inventor 王琦张云龙
Owner 北京康泰正茂农业科技股份有限公司
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