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Bacillus amyloliquefaciens with inorganic phosphorus degrading and bacteria inhibiting functions and application of bacillus amyloliquefaciens

A technology of amylolytic spores and inorganic phosphorus, which is applied in the field of agricultural microorganisms and can solve problems such as high production costs, high energy consumption, and inability for plants to absorb and utilize them

Active Publication Date: 2017-02-15
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the quick-acting phosphate fertilizers used have high production costs and high energy consumption, and the utilization rate of the applied phosphate fertilizers is only 10% to 25%. Phosphorus and Ca in soil 2+ , Fe 2+ , Fe 3+ 、Al 3+ etc. combined to form insoluble phosphate, which cannot be absorbed and utilized by plants, and brings a series of problems such as soil compaction, environmental pollution, and destruction of biodiversity.

Method used

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  • Bacillus amyloliquefaciens with inorganic phosphorus degrading and bacteria inhibiting functions and application of bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens with inorganic phosphorus degrading and bacteria inhibiting functions and application of bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens with inorganic phosphorus degrading and bacteria inhibiting functions and application of bacillus amyloliquefaciens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Preparation of Bacillus amyloliquefaciens PHODB35 microbial inoculum

[0058] Follow the steps below:

[0059] (1) Strain activation: The strain PHODB35 stored at -80°C (Bacillus amyloliquefaciens strain PHODB35 has been deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee on September 26, 2016, and the deposit number is CGMCCNo.13040 ) On LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) for activation (30℃), pick a single colony in LB slant medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) cultured at 30°C for 12 hours to obtain activated strains;

[0060] (2) Preparation of seed solution: Prepare LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 100...

Embodiment 2

[0063] Example 2 Qualitative determination test of the ability of Bacillus amyloliquefaciens PHODB35 to degrade inorganic phosphorus

[0064] Proceed as follows:

[0065] Use a sterilized toothpick to spot inoculate the PHODB35 strain activated in step (1) of Example 1 on an inorganic phosphorus-dissolving plate medium (its composition and weight ratio are: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl0.2g, Ca 3 (PO 4 ) 2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g, 20.0g agar, 1000mL distilled water, pH: 7.0-8.0), placed in a 30°C constant temperature incubator for 5 days, and measured the diameter of the transparent circle and the diameter of the colony.

[0066] Results Bacillus amyloliquefaciens PHODB35 contained Ca 3 (PO 4 ) 2 A transparent circle with a diameter of 13.0 mm is produced on the inorganic phosphorus plate medium, indicating that the Bacillus amyloliquefaciens PHODB35 can degrade inorganic phosphorus Ca well. 3 (PO 4 ) 2 , Has the potential to deg...

Embodiment 3

[0067] Example 3 Quantitative determination test of the ability of Bacillus amyloliquefaciens PHODB35 to degrade inorganic phosphorus

[0068] The experiment was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences in early August 2015. Proceed as follows:

[0069] (1) Preparation of fermentation medium: 10.0g glucose, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.2g, Ca 3 (PO 4 ) 2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g is added to 1000mL of distilled water and mixed evenly to obtain a fermentation medium (pH: 7.0-8.0). Put 50mL of fermentation medium into a 300mL conical flask, sterilize it under high temperature and high pressure, and set aside.

[0070] (2) Preparation of fermentation broth: the PHODG35 strain seed solution and blank control culture broth (LB liquid medium without inoculation of PHODG35 strain) obtained in step (2) of Example 1 were respectively inoculat...

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Abstract

The invention discloses a PHODB35 bacteria strain of the bacillus amyloliquefaciens, and the bacteria strain is preserved in the common microorganism center of China Committee for Culture Collection of Microorganisms with the preservation number of CGMCC No. 13040. The invention further discloses a microorganism bacterium agent produced by the aid of the PHODB35 bacteria strain. The PHODB35 of the bacillus amyloliquefaciens is good in inorganic phosphorus degrading, good in fertilizer effect, good in bacterial inhibiting effect of three kinds of pathogenic bacteria of cotton verticillium wilt, wilt and fusarium patch and wide in antimicrobial spectrum; the bacillus amyloliquefaciens causes no pollution problem to safety of human and livestock; the bacillus amyloliquefaciens is simple in preparation method, low in cost and easy to use.

Description

Technical field [0001] The invention belongs to the field of agricultural microorganisms, and specifically relates to a Bacillus amyloliquefaciens with the dual functions of degrading inorganic phosphorus and antibacterial, and a microbial agent containing the Bacillus amyloliquefaciens, and also relates to their use in promoting crop growth and preventing diseases application. Background technique [0002] Phosphorus is one of the essential nutrients during plant growth. The total phosphorus content in soil in my country is considerable, but more than 95% is in the form of ineffective phosphorus such as stable aluminosilicate and apatite, which is difficult for plants to directly absorb and utilize. Therefore, most crops must be topdressed with phosphate fertilizer. The current application of quick-acting phosphate fertilizers has a high production cost and high energy consumption. The utilization rate of the applied phosphate fertilizers was only 10% to 25%. Part of the phosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 李社增鹿秀云马平郭庆港张晓云
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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