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Bacillus amyloliquefaciens capable of degrading inorganic phosphorus and inhibiting bacteria and its application

A technology for dissolving starch spores and bacilli, applied in the field of agricultural microorganisms, can solve problems such as polluted environment, destruction of biodiversity, and inability of plants to absorb and utilize

Active Publication Date: 2019-09-13
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the quick-acting phosphate fertilizers used have high production costs and high energy consumption, and the utilization rate of the applied phosphate fertilizers is only 10% to 25%. Phosphorus and Ca in soil 2+ , Fe 2+ , Fe 3+ 、Al 3+ etc. combined to form insoluble phosphate, which cannot be absorbed and utilized by plants, and brings a series of problems such as soil compaction, environmental pollution, and destruction of biodiversity.

Method used

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  • Bacillus amyloliquefaciens capable of degrading inorganic phosphorus and inhibiting bacteria and its application
  • Bacillus amyloliquefaciens capable of degrading inorganic phosphorus and inhibiting bacteria and its application
  • Bacillus amyloliquefaciens capable of degrading inorganic phosphorus and inhibiting bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of embodiment 1 bacillus amyloliquefaciens PHODB35 microbial bacterial agent

[0058] Follow the steps below:

[0059] (1) Strain activation: The strain PHODB35 (Bacillus amyloliquefaciens strain PHODB35) stored at -80°C was deposited in the General Microbiology Center of China Committee for Microbial Culture Collection on September 26, 2016, and the preservation number is CGMCCNo.13040 ) on the LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) to activate (30 ℃), pick a single colony on LB slant medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) was cultivated at 30°C for 12 hours to obtain an activated bacterial strain;

[0060] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sod...

Embodiment 2

[0063] Example 2 Qualitative determination test of Bacillus amyloliquefaciens PHODB35 degrading inorganic phosphorus ability

[0064] Proceed as follows:

[0065] With sterilized toothpick, the PHODB35 bacterial strain activated in the embodiment 1 step (1) is spot-inoculated on the solution inorganic phosphorus plate culture medium (its composition and its weight ratio are: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, Ca 3 (PO 4 ) 2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g, 20.0g agar, 1000mL distilled water, pH: 7.0-8.0), placed in a constant temperature incubator at 30°C for 5 days, and measured the diameter of the transparent circle and the diameter of the colony.

[0066] Results Bacillus amyloliquefaciens PHODB35 contained Ca 3 (PO 4 ) 2 Produce a transparent circle with a diameter of 13.0 mm on the inorganic phosphorus plate medium, indicating that Bacillus amyloliquefaciens PHODB35 of the present invention can well degrade inor...

Embodiment 3

[0067] Example 3 Bacillus amyloliquefaciens PHODB35 Degradation Inorganic Phosphorus Ability Quantitative Determination Test

[0068] This experiment was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences in early August 2015. Proceed as follows:

[0069] (1) Preparation of fermentation medium: 10.0 g of glucose, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.2g, Ca 3 (PO 4 ) 2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g was added to 1000mL of distilled water, and mixed evenly to obtain a fermentation medium (pH: 7.0-8.0). Put 50mL of fermentation medium into a 300mL Erlenmeyer flask, sterilize at high temperature and high pressure, and set aside.

[0070] (2) fermentation broth preparation: the PHODG35 bacterial strain seed liquid and the blank control culture fluid (the LB liquid culture medium that does not inoculate the PHODG35 bacterial strain) of embodimen...

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Abstract

The invention discloses a Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain PHODB35, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 13040. The invention also discloses a microbial agent produced by using the PHODB35 bacterial strain. The Bacillus amyloliquefaciens PHODB35 of the present invention has a good effect on degrading inorganic phosphorus and produces high fertilizer efficiency; The bacteriostasis effect is good and the bacteriostasis spectrum is wide; the microbial agent of the invention is safe for humans and animals, and there is no problem of environmental pollution; the preparation method of the agent of the invention is simple, the cost is low, and the use is simple.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a Bacillus amyloliquefaciens with dual functions of degrading inorganic phosphorus and inhibiting bacteria, and a microbial agent containing the Bacillus amyloliquefaciens, and also relates to their effects in promoting crop growth and disease prevention. application. Background technique [0002] Phosphorus is one of the essential nutrients for plant growth. The total phosphorus content in my country's soil is considerable, but more than 95% exists in the form of stable aluminosilicate and apatite and other invalid phosphorus, which is difficult for plants to directly absorb and utilize. Therefore, most crops must be topdressed with phosphorus fertilizer. Currently, the quick-acting phosphate fertilizers used have high production costs and high energy consumption, and the utilization rate of the applied phosphate fertilizers is only 10% to 25%. Phosphoru...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 李社增鹿秀云马平郭庆港张晓云
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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