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Bacillus amyloliquefaciens and its bacterial agent with degrading inorganic phosphorus and antibacterial effect

A technology of amylolytic spores and bacilli, which is applied in the field of agricultural microorganisms and can solve problems such as water pollution, environmental pollution, and high energy consumption

Active Publication Date: 2019-10-22
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the quick-acting phosphate fertilizer mainly used has high production cost and high energy consumption, and the utilization rate of the applied phosphate fertilizer is only 10%-25%. Some of the phosphate fertilizer flows into rivers and lakes with rainwater, causing eutrophication of water bodies and causing water pollution; Part of phosphorus and Ca in soil 2+ , Fe 2+ , Fe 3+ 、Al 3+ etc. combined to form insoluble phosphate, which cannot be absorbed and utilized by plants, and brings a series of serious problems such as soil compaction, environmental pollution, and destruction of biodiversity.

Method used

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  • Bacillus amyloliquefaciens and its bacterial agent with degrading inorganic phosphorus and antibacterial effect
  • Bacillus amyloliquefaciens and its bacterial agent with degrading inorganic phosphorus and antibacterial effect
  • Bacillus amyloliquefaciens and its bacterial agent with degrading inorganic phosphorus and antibacterial effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The preparation of embodiment 1 bacillus amyloliquefaciens PHODG36 microbial bacterial agent

[0058] Follow the steps below:

[0059] (1) Strain activation: the Bacillus amyloliquefaciens strain PHODG36 stored at -80°C (the strain was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on September 26, 2016, and the preservation number is CGMCCNo.13041 ) on the LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) to activate (30 ℃), pick a single colony on LB slant medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) was cultivated at 30°C for 12 hours to obtain an activated bacterial strain;

[0060] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract ...

Embodiment 2

[0063] Embodiment 2 Bacillus amyloliquefaciens PHODG36 degrades inorganic phosphorus qualitative test

[0064] Proceed as follows:

[0065] The bacterial strain PHODG36 activated in the embodiment 1 step (1) is spot-inoculated on the inorganic phosphorus plate medium (its composition and weight ratio thereof are: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, Ca 3 (PO 4 ) 2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g, 20.0g agar, 1000mL distilled water, pH: 7.0-8.0), placed in a constant temperature incubator at 30°C for 5 days, and measured the diameter of the transparent circle and the diameter of the colony.

[0066] Results Bacillus amyloliquefaciens PHODG36 contained Ca 3 (PO 4 ) 2 A transparent circle with a diameter of 12.0 mm was produced on the inorganic phosphorus plate medium, indicating that Bacillus amyloliquefaciens PHODG36 can well degrade inorganic phosphorus Ca 3 (PO 4 ) 2 , has the potential to degrade inorganic phospho...

Embodiment 3

[0067] Example 3 Quantitative Determination Test of Bacillus amyloliquefaciens PHODG36 Degradation Inorganic Phosphorus Ability

[0068] This experiment was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences in early August 2015. Proceed as follows:

[0069] (1) Preparation of fermentation medium: 10.0 g of glucose, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.2g, Ca 3 (PO 4 ) 2 5.0g, KCl 0.2g, MnSO 4 0.03g, FeSO 4 0.003g was added to 1000mL of distilled water, and mixed evenly to obtain a fermentation medium (pH: 7.0-8.0). Put 50 mL of fermentation medium into a 300 mL Erlenmeyer flask, and sterilize under high temperature and high pressure.

[0070] (2) fermentation broth preparation: the PHODG36 bacterial strain seed liquid and the blank control culture fluid (the LB liquid culture medium that does not inoculate the PHODG36 bacterial strain) of embodiment 1...

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Abstract

The invention discloses a Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain PHODG36, which has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No. 13041. The invention also discloses the microbial bacterial agent produced by using PHODG36. It also discloses the application of PHODG36 in degrading inorganic phosphorus, promoting crop growth and preventing and controlling cotton diseases. The PHODG36 bacterial strain of the present invention has a good effect on degrading inorganic phosphorus and high fertilizer efficiency; at the same time, it has a good antibacterial effect and a wide antibacterial spectrum, and has a good inhibitory effect on three pathogenic bacteria of cotton verticillium wilt, fusarium wilt and blight; in addition, the present invention The inventive microbial bacterial fertilizer is safe for humans and animals, and has no environmental pollution problem; the inventive bacterial agent has simple preparation method, low cost and simple use.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a Bacillus amyloliquefaciens with dual functions of degrading inorganic phosphorus and inhibiting bacteria, and a microbial agent containing the Bacillus amyloliquefaciens, and also relates to their effects in promoting crop growth and disease prevention. use. Background technique [0002] Phosphorus is one of the essential nutrients for plant growth. The total phosphorus content in my country's soil is considerable, but more than 95% exists in the form of stable aluminosilicate and apatite and other invalid phosphorus, which is difficult for plants to directly absorb and utilize. Therefore, most crops must be topdressed with phosphorus fertilizer. At present, the quick-acting phosphate fertilizer mainly used has high production cost and high energy consumption, and the utilization rate of the applied phosphate fertilizer is only 10%-25%. Some of the phosp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/07
Inventor 马平李社增鹿秀云郭庆港张晓云
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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