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Anti-Cotinus coggygria oxysporum strain and use thereof

A technology of cotinus fusarium wilt and fusarium wilt fungus, applied in the field of microorganisms, can solve problems such as difficulty in preventing and controlling cotinus fusarium wilt, difficult control, serious environmental pollution and the like

Active Publication Date: 2015-09-02
BEIJING FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a bacterial strain antagonizing Cotinus wilt and its application in the prevention and treatment of Cotinus fusarium aiming at the technical problem that the phytopathogenic bacteria that exist in plant disease control produce drug resistance. The bacterial strain of the present invention is obtained from saline-alkali The biocontrol bacterial strains obtained by separation and screening in the soil with high-efficiency antagonism against Cotinus Fusarium Wilt can efficiently inhibit the growth of Cotinus Cotinus Fusarium Wilt and Fusarium oxysporum, and can obviously inhibit the production of micro-sclerotia of Cotinus Cotinus Fusarium wilt, applying the method of the present invention The strain of the antagonistic bacteria Bacillus subtilis C-2-3-2 solves the current difficulty in the prevention and control of Cotinus subtilis with chemical pesticides, agricultural measures, and physical methods. It is easy to cause serious environmental pollution and the existing technology of biological control is blank

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  • Anti-Cotinus coggygria oxysporum strain and use thereof
  • Anti-Cotinus coggygria oxysporum strain and use thereof
  • Anti-Cotinus coggygria oxysporum strain and use thereof

Examples

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Effect test

Embodiment 1

[0071] Example 1 Isolation, screening and identification of Bacillus subtilis C-2-3-2

[0072] 1. Isolation and screening of Bacillus subtilis C-2-3-2

[0073] The bacterial strain of the present invention is isolated from the soil of the saline-alkali land control experimental area of ​​the Research Center of the Chinese Academy of Forestry in Dagang, Binhai, Tianjin.

[0074] 1A) Take 5g of saline-alkali soil, put it into a sterilized Erlenmeyer flask filled with 45mL of sterile water, place it on a shaker, shake it at 28°C and 200rpm for 30min, and let it stand to obtain a bacterial suspension (that is, 10 -1 The soil dilution solution, that is, the soil solution diluted 10 times); Then adopt 10 times of gradient dilution method to dilute successively to obtain 10 -6 、10 -7 、10 -8 Concentration gradient dilution, take 0.1mL from each concentration gradient suspension, and spread it on the LB medium plate with a sterile applicator, and use sterile water as a control, spre...

Embodiment 2

[0105] Example 2 Exploration of Biological Characteristics of Bacillus subtilis C-2-3-2

[0106] 1A) Pick a circle of activated Bacillus subtilis C-2-3-2 strains and inoculate them in 40 mL of NB liquid medium (each 1000 mL contains 3 g of beef extract, 10 g of peptone, 5 g of NaCl, 1000 mL of water, pH 7.0 -7.2) in a 100mL Erlenmeyer flask, and then placed on a shaker, at 28°C, 200rpm shaking culture for 1d, to prepare the Bacillus subtilis C-2-3-2 strain seed liquid, wherein, Bacillus subtilis C-2-3- OD after 10-fold dilution of the seed solution of the 2 strains 600 The value is about 0.27;

[0107] 1B) Bacillus subtilis C-2-3-2 bacterial strain seed solution is inserted in different Erlenmeyer flasks (100mL) according to the inoculation amount of 1% (V / V), and the different salts of 40mL are housed in different Erlenmeyer flasks ( NaCl) concentration, pH value of the NB liquid medium, and then placed on a shaker, at 28 ° C, 200 rpm under the condition of shaking culture ...

Embodiment 3

[0113] Example 3 Antagonistic effect of Bacillus subtilis C-2-3-2 bacterial liquid on Cotinus wilt and Fusarium oxysporum

[0114] 1A) Pick a Bacillus subtilis C-2-3-2 strain after the activation of a bacterial ring, and inoculate it in a conical flask (250 mL), which contains 100 mL of LB liquid medium (each 1000 mL contains 10 g of tryptone , yeast extract 5g, NaCl 10g, water 1000mL, pH 7.0-7.2), then put the Erlenmeyer flask on a shaker, and shake it at 28°C and 200rpm for 1d to prepare the Bacillus subtilis C-2-3-2 strain Seed liquid (i.e. C-2-3-2 bacterial liquid), wherein, the OD of Bacillus subtilis C-2-3-2 bacterial liquid diluted 10 times 600 The value is about 0.37, and this bacterial solution is the bacterial solution used for the determination of antagonism, and it is reserved;

[0115] 1B) Take the C-2-3-2 bacterial solution prepared in step 1A) and add it to the PDA plate solid medium cooled to about 45°C after sterilization, wherein the volume ratio of the bact...

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Abstract

The invention discloses an anti-Cotinus coggygria oxysporum strain and a use thereof. The anti-Cotinus coggygria oxysporum strain is Bacillus subtilis C-2-3-2 and has a microbial accession number of CGMCC NO. 9369. The Bacillus subtilis C-2-3-2 is separated from saline land soil, can prevent Verticillium wilt of Cotinus coggygria, and has good effects of inhibiting Cotinus coggygria oxysporum and fusarium oxysporum. The sterile filtrate of the Bacillus subtilis C-2-3-2 can obviously inhibit Cotinus coggygria oxysporum microsclerotia formation. The Bacillus subtilis C-2-3-2 is a biological control potential bacterial strain with high prevention effects, special prevention and treatment effects and environmental safety, and has a good development and application prospect.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a bacterial strain antagonizing plant pathogenic fungi and application thereof, which belongs to the field of microorganisms and can be used for plant protection and prevention and control of plant diseases. Background technique [0002] Sumac, also known as Cotinus zodiac or Cotinus sumac, is a shrub or small tree with a plant height of 3-5m, belonging to the genus Sumac in the Anacardaceae family. Cotinus cotinus is an excellent autumn red leaf tree species, and it is also a common barren hill greening tree species in the Yangtze River Basin, North China, and Central China. It has the characteristics of strong growth and resistance to barrenness. After the autumn frost, the leaves of sumac are bright red, which is a tree species with high economic value and ornamental value. The Fragrant Hills, Badaling, Badachu and other places in Beijing are planted, among which the most famous is the "...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/125
Inventor 田呈明黄华毅王永林孙妍于地美
Owner BEIJING FORESTRY UNIVERSITY
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