5 results about "Rhus cotinus" patented technology

The application discloses application of a Cotinus coggygria gene CcDT1 to improving drought resistance of plants and belongs to the field of genetic engineering. The CcDT1 gene from the Cotinus coggygria is used to construct an overexpression vector, the expression vector containing the gene is obtained by genetic transformation, and the overexpression Arabidopsis thaliana plant is obtained, and the overexpression plant is subjected to drought stress treatment. The experimental results show that, after being treated for 2 weeks under the drought condition, the overexpression plant has higher drought resistance than the wild type plant, the total chlorophyll content is higher, and the ion permeation rate and the water loss rate are lower, indicating that the overexpression of the Cotinus coggygria gene CcDT1 can significantly improve the drought resistance of the plant. The gene can provide an important theoretical basis for breeding drought-resistant forest trees and has important scientific significance in strengthening the diversity of forest tree species.

The application discloses a drought-inducible promoter of Cotinus coggygria and application thereof, a DNA sequence of the promoter is shown as SEQ ID No. 1, proCcDT1 is cloned from a promoter region of a CcDT1 gene of the Cotinus coggygria and is verified through experiments. First, elements of the promoter region are analyzed, and the result shows that the elements contain multiple cis-acting elements that can be involved in response to abiotic stress. Then, proCcDT1-GUS transgenic plants are treated through 10% PEG simulation drought, and it is found that GUS staining of the transgenic plants is enhanced, so it can be concluded that the GUS expression level driven by the promoter is increased under drought induction conditions, and it is also proved that the promoter can be induced by drought stress. The promoter proCcDT1 is introduced into an Arabidopsis genome through genetic transformation technology, so that the promoter can be stably induced by drought stress in a heterologous plant, and the practical application has important value for cultivating drought-resistant Cotinus coggygria varieties.

The application belongs to the technical field of plant disease detection, and particularly relates to a primer, a probe, a kit, a method and application for detecting the Fusarium oxysporum f. sp. multivorum based on RPA-LFD technology, a nucleotide sequence of a forward primer in the primer group is shown as SEQ ID No. 1, a nucleotide sequence of a reverse primer is shown as SEQ ID No. 2, and a nucleotide sequence of the probe is shown as SEQ ID No. 4. The application adopts the RPA-LFD method to detect the Fusarium oxysporum f. sp. multivorum for the first time, has the characteristics of strong specificity, high sensitivity, rapidness, convenience, and independence on large-scale instruments and equipment, and can be applied to field detection of the wild Cotinus coggygria sample.

This invention provides the application of a *Trichoderma harzianum* strain Th01 in the control of *Cotinus coggygria* wilt disease. The *Trichoderma harzianum* strain was isolated in April 2025 from the rhizosphere soil of healthy *Cotinus coggygria* plants in a control plot at Xiangshan Park, Beijing. It exhibits targeted control of *Cotinus coggygria* wilt disease, shows significant inhibitory effects against *Verticillium dahliae*, promotes root repair while secreting auxin, and possesses the ability to colonize directionally in the rhizosphere soil of diseased *Cotinus coggygria* plants and continuously inhibit the germination of pathogenic microsclerotia. The *Trichoderma harzianum* strain... Trichoderma harzianum It was deposited on November 7, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42309. It can be used to control Fusarium wilt in Cotinus coggygria and can be made into a fermentation broth for the control of Fusarium wilt in Cotinus coggygria, showing good application prospects.

This invention provides the application of Bacillus subtilis strain Bs25 in the control of Fusarium wilt in Cotinus coggygria. The Bacillus subtilis strain was isolated in April 2025 from the rhizosphere soil of healthy Cotinus coggygria plants in a control plot at Xiangshan Park, Beijing. It exhibits targeted control of Fusarium wilt in Cotinus coggygria, showing a significant inhibitory effect on Verticillium dahliae. While secreting auxin, it promotes root repair and possesses the ability to colonize the rhizosphere soil of diseased Cotinus coggygria plants and continuously inhibit the germination of pathogenic microsclerotia. The Bacillus subtilis strain was deposited on November 7, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36538. Its microbial inoculum remains active under high temperature, high salinity, strong alkalinity, or extreme drought conditions. It can be directly used to control Fusarium wilt in Cotinus coggygria and has promising application prospects.