12 results about "Rhus cotinus" patented technology

The present invention relates to the technical field of compound extraction, discloses a kind of extraction process of fisetin in cotinus coggygria, including: S1: take the branch and / or leaves of cotinus coggygria and crush them to obtain cotinus coggygria crushed material;S2: cotinus coggygria crushed material is added into a continuous countercurrent extraction tank, water is added, extraction is started, and extraction temperature is 90-95 DEG C to obtain extract;The extract is filtered and filtrate is taken, heated and concentrated to obtain a crude extract;S3: the crude extract is rinsed, filter and take filter cake, obtain solid, solid is added to ethanol for dissolution, after adding water and filtering, reduced pressure is concentrated to ethanol concentration of 60%-75%, crystallization, filter and take filter cake, obtain refined extract;S4: ethanol is added to the refined extract, heated to dissolve and continue 1-1.5h, filter and take filtrate, after reduced pressure is concentrated to ethanol concentration of 70%-75%, stand crystallization filtration and drying, obtain fisetin finished product. The extraction process of the present invention has high extraction efficiency, large conversion rate and is green and environmentally friendly.

The application discloses application of a Cotinus coggygria gene CcDT1 to improving drought resistance of plants and belongs to the field of genetic engineering. The CcDT1 gene from the Cotinus coggygria is used to construct an overexpression vector, the expression vector containing the gene is obtained by genetic transformation, and the overexpression Arabidopsis thaliana plant is obtained, and the overexpression plant is subjected to drought stress treatment. The experimental results show that, after being treated for 2 weeks under the drought condition, the overexpression plant has higher drought resistance than the wild type plant, the total chlorophyll content is higher, and the ion permeation rate and the water loss rate are lower, indicating that the overexpression of the Cotinus coggygria gene CcDT1 can significantly improve the drought resistance of the plant. The gene can provide an important theoretical basis for breeding drought-resistant forest trees and has important scientific significance in strengthening the diversity of forest tree species.

The invention relates to an application of a cotinus coggygria xylem extract in preparation of a neuroprotective drug. The traditional cognitive limitation of clearing heat and promoting diuresis of cotinus coggygria is broken through for the first time, the cotinus coggygria xylem extract with neuroprotective activity is disclosed, the extract can remarkably improve the survival rate of injured cells and shows broad-spectrum and remarkable neuroprotective activity, the preparation process is simple, reproducibility is good, large-scale production is easy, and the cotinus coggygria xylem extract is suitable for industrial production. A novel candidate substance and a preparation method easy to industrialize are provided for development of drugs for treating neurodegenerative diseases, and the compound has a good industrial transformation prospect.

The application discloses a drought-inducible promoter of Cotinus coggygria and application thereof, a DNA sequence of the promoter is shown as SEQ ID No. 1, proCcDT1 is cloned from a promoter region of a CcDT1 gene of the Cotinus coggygria and is verified through experiments. First, elements of the promoter region are analyzed, and the result shows that the elements contain multiple cis-acting elements that can be involved in response to abiotic stress. Then, proCcDT1-GUS transgenic plants are treated through 10% PEG simulation drought, and it is found that GUS staining of the transgenic plants is enhanced, so it can be concluded that the GUS expression level driven by the promoter is increased under drought induction conditions, and it is also proved that the promoter can be induced by drought stress. The promoter proCcDT1 is introduced into an Arabidopsis genome through genetic transformation technology, so that the promoter can be stably induced by drought stress in a heterologous plant, and the practical application has important value for cultivating drought-resistant Cotinus coggygria varieties.

The invention discloses application of a cotinus coggygria gene CcDT1 to improvement of drought resistance of plants, and belongs to the field of gene engineering. The method comprises the following steps: constructing an overexpression vector by using a CcDT1 gene from cotinus coggygria, carrying out genetic transformation on the constructed expression vector containing the gene to obtain an overexpression arabidopsis thaliana plant, and carrying out drought stress treatment on the overexpression plant. Experimental results show that after the cotinus coggygria gene CcDT1 is treated for 2 weeks under the drought condition, compared with a wild plant, an overexpressed strain shows higher drought tolerance, the total chlorophyll content is higher, the ion permeability and the water loss rate are lower, and it is indicated that the overexpressed cotinus coggygria gene CcDT1 can remarkably improve the drought tolerance of the plant. The gene can provide an important theoretical basis in drought-resistant forest tree breeding work, and also has important scientific significance in enhancing the diversity of forest tree species.

The application belongs to the technical field of plant disease detection, and particularly relates to a primer, a probe, a kit, a method and application for detecting the Fusarium oxysporum f. sp. multivorum based on RPA-LFD technology, a nucleotide sequence of a forward primer in the primer group is shown as SEQ ID No. 1, a nucleotide sequence of a reverse primer is shown as SEQ ID No. 2, and a nucleotide sequence of the probe is shown as SEQ ID No. 4. The application adopts the RPA-LFD method to detect the Fusarium oxysporum f. sp. multivorum for the first time, has the characteristics of strong specificity, high sensitivity, rapidness, convenience, and independence on large-scale instruments and equipment, and can be applied to field detection of the wild Cotinus coggygria sample.

The invention discloses a cotinus coggygria drought inducible promoter and application thereof, the DNA sequence of the promoter is as shown in SEQ ID No.1, proCcDT1 is cloned from a cotinus coggygria CcDT1 gene promoter region, and experimental verification is carried out. Firstly, promoter region elements are analyzed, and a result shows that the promoter region elements contain a plurality of cis-acting elements possibly participating in response to abiotic stress. Afterwards, a proCcDT1-GUS transgenic plant is subjected to 10% PEG (polyethylene glycol) simulated drought treatment, and the GUS dyeing degree of the transgenic plant is enhanced, so that the GUS expression level driven by the promoter under a drought induction condition is increased, and the promoter is proved to be induced by drought stress. The promoter proCcDT1 is introduced into an arabidopsis thaliana genome through a genetic transformation technology, so that the promoter proCcDT1 can be stably induced by drought stress in heterologous plants, and the practical application of the promoter proCcDT1 has important value for cultivating drought-tolerant cotinus coggygria varieties.

This invention discloses a strain of *Bacillus amyloliquefaciens*, JDF630, and its application in controlling *Fusarium wilt* of *Cotinus coggygria*, belonging to the field of microbial technology. The *Bacillus amyloliquefaciens* strain JDF630 described in this invention was deposited on July 13, 2022, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC NO. 25289. This invention demonstrates that strain JDF630 exhibits broad-spectrum inhibitory activity against various plant pathogens. Treatment with JDF630 significantly reduces the damage caused by *Fusarium wilt* to *Cotinus coggygria*, providing a new microbial strain and control strategy for the prevention and control of plant pathogens, demonstrating excellent biological control effects.

The invention relates to the technical field of pet food, in particular to a compound additive for pet aging resistance. The composite additive for pet aging resistance is prepared from the following raw materials in parts by weight: 20 to 25 parts of nicotinamide, 10 to 15 parts of cotinus coggygria extract, 20 to 25 parts of wheat germ extract, 65 to 100 parts of hydrolyzed fish protein powder and 1 to 2 parts of natural xanthophyll. The composite additive disclosed by the invention has the synergistic anti-aging effects of restarting energy, eliminating aging, remodeling autophagy and the like, and also has the effects of regulating immunity, resisting inflammation, protecting eyes, protecting joints and improving fur.

This invention provides the application of a *Trichoderma harzianum* strain Th01 in the control of *Cotinus coggygria* wilt disease. The *Trichoderma harzianum* strain was isolated in April 2025 from the rhizosphere soil of healthy *Cotinus coggygria* plants in a control plot at Xiangshan Park, Beijing. It exhibits targeted control of *Cotinus coggygria* wilt disease, shows significant inhibitory effects against *Verticillium dahliae*, promotes root repair while secreting auxin, and possesses the ability to colonize directionally in the rhizosphere soil of diseased *Cotinus coggygria* plants and continuously inhibit the germination of pathogenic microsclerotia. The *Trichoderma harzianum* strain... Trichoderma harzianum It was deposited on November 7, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42309. It can be used to control Fusarium wilt in Cotinus coggygria and can be made into a fermentation broth for the control of Fusarium wilt in Cotinus coggygria, showing good application prospects.

The application discloses a light substrate for seedling raising of Cotinus coggygria with red and bright leaf color, and a preparation method and a fertilization method thereof, and comprises the following steps: preparing the light substrate by using the following raw materials: yellow heart soil, citrus peel residue organic fertilizer, vermiculite, perlite and high-nitrogen compound fertilizer; the citrus peel residue organic fertilizer is used in the preparation of the substrate, the PH is controlled to be 7.0-8.2, and calcium superphosphate is added; the content of various pigments of Cotinus coggygria is controlled, and the color quality is improved through the three factors; and different fertilization schemes are formulated for each period in the later period of fertilization. The application has the characteristics of being specific, simple and easy to operate, the light substrate seedlings are prepared, the light substrate seedlings are fertilized and managed in the later period, and finally the effect of improving the leaf color quality of Cotinus coggygria is realized, the ornamental period is prolonged, the leaf color is bright and beautiful at the ornamental period, and the ornamental value of Cotinus coggygria is improved.

This invention provides the application of Bacillus subtilis strain Bs25 in the control of Fusarium wilt in Cotinus coggygria. The Bacillus subtilis strain was isolated in April 2025 from the rhizosphere soil of healthy Cotinus coggygria plants in a control plot at Xiangshan Park, Beijing. It exhibits targeted control of Fusarium wilt in Cotinus coggygria, showing a significant inhibitory effect on Verticillium dahliae. While secreting auxin, it promotes root repair and possesses the ability to colonize the rhizosphere soil of diseased Cotinus coggygria plants and continuously inhibit the germination of pathogenic microsclerotia. The Bacillus subtilis strain was deposited on November 7, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36538. Its microbial inoculum remains active under high temperature, high salinity, strong alkalinity, or extreme drought conditions. It can be directly used to control Fusarium wilt in Cotinus coggygria and has promising application prospects.