Bacillus subtilis and application thereof

A Bacillus subtilis, bs-03cgmccno.3038 technology, applied in the field of microorganisms, can solve the problems of excessive pesticide residues, affecting the quality of life, environmental pollution and the like

Active Publication Date: 2010-02-10
山东泰宝生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to excessive application and abuse of pesticides, soil fertility decline and environmental pollution
E

Method used

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  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof
  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] 1. Isolation and purification of Bacillus subtilis Bs-03 CGMCC No.3038

[0012] Bacillus subtilis (Bacillus subtilis) Bs-03 CGMCC No.3038 was isolated from the experimental nursery soil of Shandong Academy of Forestry Sciences by soil dilution separation method.

[0013] (1) Soil sample collection: sample 200g from the soil surface to 15cm, take several samples at one location, mix them through a coarse sieve, and take 25g for separation;

[0014] (2) Medium plate preparation: NA (nutrient agar) medium and PDA (potato dextrose agar) medium are melted, poured into sterilized culture dish respectively, cooling, make NA and PDA plate, described NA medium formula Be peptone 10g, beef extract 3g, NaCl 5g, agar 15g, distilled water 1000mL; Described PDA medium formula is potato (peeled and cut into pieces) 300g, glucose 20g, agar 20g, distilled water 1000mL.

[0015] (3) Weigh 1g of soil sample into a sterilized test tube, add 100mL of sterile water, shake, and make 10 -2 S...

Embodiment 2

[0023] Example 2: Bacillus subtilis (Bacillus subtilis) Bs-03 CGMCC No.3038 fermentation NB medium (commercially available) formula: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1L.

[0024] NYBD medium formula: beef extract 8g, yeast extract 5g, glucose 10g, sodium chloride 5g, water 1000mL, pH7.3.

[0025] The mass fermentation culture formula (percentage by weight) is: soybean flour 1.8%, corn flour 1.6%, starch 0.44%, calcium carbonate 0.44%, fish meal 0.47%, magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.03%, the rest is water, pH 7.5.

[0026] 1. Activation of Bacillus subtilis (Bacillus subtilis) Bs-03 CGMCC No.3038 strain Inoculate the strain preserved on the slant to the slant of the NA test tube and incubate at 28°C for 24h.

[0027] 2. Preparation of Bacillus subtilis Bs-03 CGMCC No.3038 seed solution

[0028] Pick tricyclic bacteria from the inclined surface and inoculate them into the Erlenmeyer flask containing NB culture solutio...

Embodiment 3

[0033] Embodiment 3: the application of Bacillus subtilis (Bacillus subtilis) Bs-03 CGMCC No.3038 fermented liquid

[0034] In order to clarify the effect of Bacillus subtilis (Bacillus subtilis) Bs-03 CGMCC No.3038 fermentation broth on cotton Verticillium wilt control and yield increase, and to provide a basis for its popularization and application. We conducted this test at the Dongying test base from May to June 2008, and the test results are reported as follows:

[0035] 1 Test materials and methods

[0036] 1.1 Drugs to be tested

[0037] Bacillus subtilis (Bacillus subtilis) Bs-03 CGMCC No.3038 fermented liquid produced by the fermentation method of the present invention; 50% carbendazim wettable powder (produced by Jiangsu Xinyi Pesticide Co., Ltd., commercially available).

[0038] 1.2 Test crops and control objects: the test crops are cotton, and the variety is Zhonghua 17; control objects: Verticillium wilt

[0039] 1.3 Conditions of the test site

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Abstract

The invention discloses a strain of bacillus subtilis and application thereof. The bacillus subtilis is Bs-03 CGMCC No. 3038 and can be used as a biological fertilizer and a biological pesticide whichcan prevent and control various diseases of plants. Experiments indicate that fermentation liquid of the bacillus subtilis Bs-03 CGMCC No. 3038 has significant effect on preventing and controlling verticillium dahliae in cotton, and cotton grows vigorously without causing phytotoxicity because of using the bacillus subtilis Bs-03 CGMCC No. 3038.

Description

technical field [0001] The invention relates to a bacillus subtilis and application thereof, belonging to the field of microorganisms. Background technique [0002] The planting industry causes huge losses due to pests and diseases every year. There are many ways to control pests and diseases, and chemical control plays an important role in the history of agricultural development in the world. For centuries, chemical pesticides have played a huge role in the control of plant diseases and insect pests, and have made important contributions to increasing, stabilizing and improving agricultural production. However, due to excessive application and abuse of pesticides, soil fertility decline and environmental pollution. Especially in recent years, due to excessive pesticide residues, it has seriously affected people's quality of life. In the current pollution-free cultivation of vegetables, fruits and other crops, microbial pesticides are getting more and more attention. Bec...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/125
Inventor 周峰牛赡光王清海刘幸红张淑静郑述乾
Owner 山东泰宝生物科技股份有限公司
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