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Acinetobacter phosphate solubilizing growth-promotion bacterial strain Y40 and application thereof

A bacterium and phosphorus-dissolving technology, which is applied in the field of agricultural microorganisms, can solve the problems of phosphorus-dissolving bacteria competition and difficulty in exerting activity, and achieve efficient phosphorus-dissolving and growth-promoting functions, good growth-promoting effects, and the effect of promoting crop growth

Active Publication Date: 2015-10-14
江苏绿博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus solubilizing bacteria in the rhizosphere of plants are not enough to compete with other microorganisms, and it is difficult to exert their activity

Method used

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  • Acinetobacter phosphate solubilizing growth-promotion bacterial strain Y40 and application thereof
  • Acinetobacter phosphate solubilizing growth-promotion bacterial strain Y40 and application thereof
  • Acinetobacter phosphate solubilizing growth-promotion bacterial strain Y40 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Isolation and identification of functional strains

[0030] Cut 5g potato roots into a triangular flask filled with 45mL sterile water, shake at 170rpm for 30min to make a suspension, and then prepare a suspension of 10 -3 , 10 -4 , 10 -5 , 10 -6 Soil diluent. From 10 -4 , 10 -5 , 10 -6 Take 0.1 mL of the diluted solution and spread it on the calcium phosphate inorganic phosphorus medium and the egg yolk medium (repeated 3 times for each concentration on each medium). After separating the organic and inorganic phosphate bacteria plates and incubating them at 30°C for 2-3 days and 5-7 days respectively, take them out for observation, and select the colonies that produce transparent circles on the solid medium plates to be preliminarily identified as phosphate solubilizing bacteria. After streaking and purification , Stored in a refrigerator at 4℃ for further study.

[0031] The preparation method of the calcium phosphate inorganic phosphorus medium used is to prep...

Embodiment 2

[0033] Example 2: Production of bacterial suspension of functional bacteria

[0034] Strain Y40 (CGMCC NO.10241) was inoculated into LB broth for liquid fermentation production. The conditions for fermentation production were: pH 6.0~7.0, fermentation temperature range 30~35℃, stirring speed 170~200 rpm, fermentation time For 40h, make the amount of bacteria in the fermentation broth ≥1×10 10 Cells / mL; the infusion is the fermentation broth at 8000r / min and centrifuged for 6min, and then the cells are resuspended with an equal volume of 0.9% normal saline.

[0035] The preparation method of the LB medium used is, taking the preparation of 1L medium as an example: peptone 10g, yeast powder 5g, NaCl10g, agar 18-20g, constant volume to 1000mL, natural pH, and sterilization at 121°C for 20min.

Embodiment 3

[0036] Example 3: Greenhouse growth promotion effect test

[0037] 3.1 The application effect of growth-promoting organic fertilizer on tomatoes (season 1 and season 2)

[0038] The two-season tomato growth promotion experiment was carried out in the greenhouse of Jiangsu Zhongyi Biological Fertilizer Engineering Center. The test crops: Tomato variety Hezu 906, tomato seeds were soaked in 60℃ warm water, stirred to room temperature, precipitated seeds were taken, and disinfected with 5% sodium hypochlorite After washing 3-4 times with sterile water for 3 minutes, in a 30°C incubator, shading and accelerating germination to whiteness, then burying in the substrate, and cultivating tomato seedlings that are timely, sufficient, strong and well-proportioned. There are three treatments: 1) add decomposed chicken manure, calcium phosphate and Y40 bacteria suspension (Y40); 2) add decomposed chicken manure and calcium phosphate (CK1); 3) add decomposed chicken manure and superphosphate ( ...

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Abstract

The invention provides an acinetobacter phosphate solubilizing growth-promotion bacterial strain Y40 and application thereof. The bacterial strain belongs to potato rhizosphere and is preserved in China General Microbiological Culture Collection Center on December 25, 2014 and the preservation number is CGMCC No.10241. Flask shaking test results show that the bacterial strain can effectively reduce the pH value of a solution and dissolve insoluble phosphorus in the solution; and bacterium filling pot experiment results show that the bacterial strain can effectively promote growth of tomato and eggplant plants, meanwhile, can colonize at rhizosphere of plants in large amount and lives in roots to play the effects of growth-promotion.

Description

Technical field [0001] The invention belongs to the field of agricultural microorganisms and provides a strain of Acinetobacter phosphate-dissolving and growth-promoting bacteria Y40 and its application. Background technique [0002] Phosphorus is an essential nutrient element for plant growth. Phosphorus deficiency is common in cultivated land in my country. Invalid phosphorus forms in the soil account for 95%, and plants can hardly absorb and utilize it directly. Insoluble phosphorus in the soil can be divided into organic phosphorus and inorganic phosphorus, in which insoluble organic phosphorus accounts for 20%-50% of the total phosphorus in the soil. The effective phosphorus content of phosphate fertilizers such as superphosphate is low, and the utilization rate of phosphate fertilizer in the season after application is 5%-25%, and most of the remaining phosphorus is related to the Ca in the soil. 2+ , Fe 3+ And Al 3+ The formation of insoluble phosphate, so how to improve p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/01
Inventor 沈其荣李荣赵买琼张瑞福
Owner 江苏绿博生物科技有限公司
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