Repairing agent for microbes in water bodies of excessive culture zones

A technology for microbial restoration and breeding areas, applied in the field of applied biology, can solve problems such as inability to adapt to in-situ environmental conditions in excessive breeding areas, the degradation ability of a single microorganism, and threats to the safety of farmed fish, so as to promote mass reproduction and inhibit pathogenic microorganisms The effect of breeding and not producing drug resistance

Inactive Publication Date: 2010-12-22
HEBEI PROVINCIAL ENVIRONMENTAL SCI RES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Beneficial microorganisms are generally isolated and screened from soil and water sediments under experimental conditions. A single microorganism only has a specific degradation ability for certain organic pollutants; although genetically engineered bacteria can enhance the purification function of microbial preparations, they cannot Biosafety is an insurmountable ecological problem
[0005] After microbial preparations are put into aquaculture water bodies, their degradation activity is affected by environmental factors such as temperature and DO. Unsuitable environmental factors such as temperature, humidity, pH and DO will reduce or lose the effectiveness of microbial preparations. Some microorganisms on the market The preparation cannot adapt to the in-situ environmental conditions in the over-cultured area, so that the microorganisms in it cannot quickly become the dominant population in the water body, and the restoration effect is not obvious, and these foreign microorganisms pose a potential threat to the safety of farmed fish. Limit the application of these microorganisms in the field of microbial remediation in aquaculture areas

Method used

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  • Repairing agent for microbes in water bodies of excessive culture zones
  • Repairing agent for microbes in water bodies of excessive culture zones
  • Repairing agent for microbes in water bodies of excessive culture zones

Examples

Experimental program
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Effect test

Embodiment 1-4

[0022] Embodiment 1-4 sees the following table:

[0023]

[0024] These four strains of bacteria of the present invention are mixed with clay and zeolite powder adsorption substances according to the culture medium after conventional fermentation, and are made into granules through low-temperature compression. One gram of microecological restoration preparation contains about 10 billion bacteria. When in use, it is activated first, and the activation method is to fill some water from the water body in the over-culture area and pour it into a container for holding the preparation, let it stand overnight, and then pour it into the water body in the over-culture area the next day.

[0025] Screening, cultivation and fermentation process of four strains of the present invention

[0026] 1. Enrichment and separation of strains of the present invention

[0027] Take the experimental mud sample and place it in a plastic tank, add fish feed and sterilized fresh water, add fish fe...

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PUM

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Abstract

The invention relates to a repairing agent for microbes in water bodies of excessive culture zones, which comprises the following bacteria: Bacillus, Pseudomonas, Bacillus pumilus and Acinetobacter; the collection institution is the China General Microbiological Culture Collection Center (CGMCC); the collection numbers are respectively CGMCC No: 3737, 3739, 3752 and 3712; and the mass ratio of the Bacillus to the Pseudomonas to the Bacillus pumilus to the Acinetobacter is 1.4-1.7:1.0-1.4:0.9-1.1:1.2-1.5. The repairing agent of the invention is a microbial ecological agent which can be used for repairing excessive culture zones of water environment, is harmless to culture organisms, can inhibit propagation and growth of harmful microbes in water bodies, purifies the water quality and improves the quality and the performance of aquatic products.

Description

technical field [0001] The invention belongs to the field of applied biotechnology, and relates to a microbial restoration agent for water environment over-cultivation areas. Background technique [0002] As an ecological regulator, microorganisms can significantly improve water quality, increase dissolved oxygen, reduce ammonia nitrogen and nitrite nitrogen, inhibit the reproduction of harmful microorganisms, degrade organic matter, and have the advantages of low cost and high yield, and are increasingly valued. [0003] With the emergence of intensive aquaculture, the breeding mode is mainly based on feeding, and the breeding density has been greatly increased, resulting in the increase of residual bait, feces, nitrogen, phosphorus and other eutrophic factors in the water body, which promotes the proliferation of microorganisms (including pathogenic Bacteria), COD, ammonia nitrogen content increased, dissolved oxygen decreased, and some have exceeded the national third-cla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/07C12R1/38C12R1/01
Inventor 田在锋肖国华胡晓波高晓田陈新永王萍边蔚祝欢李艳华
Owner HEBEI PROVINCIAL ENVIRONMENTAL SCI RES
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