Method for improving output of crude oil by utilizing synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria

Abstract

The invention discloses a method for improving the output of crude oil by utilizing a synergistic effect of combined bacteria liquid containing phosphate-solubilizing microorganisms and nitrogen-fixing bacteria. The method comprises the steps: injecting diluent of a compound microorganism bacterium agent prepared from pseudomonas fermentation liquor with a phosphate-solubilizing function, azotobacter fermentation liquor and combined bacteria liquid with a petroleum hydrocarbon degrading function into an oil pool via a water injection well or injecting through a production well, and extracting oil to obtain the crude oil. The compound microorganism bacterium agent is prepared from the pseudomonas fermentation liquor, the azotobacter fermentation liquor and the combined bacteria liquid with the petroleum hydrocarbon degrading function according to a ratio of 1 to 1.2 to 1.6-8; the combined bacteria liquid with the petroleum hydrocarbon degrading function contains bacillus, acinetobacter and xanthomonas; and the three bacteria are independently cultivated into single bacteria liquid of 108-1010cfu / ml, and then mixed iso-volumetrically, so that the combined bacteria liquid with the petroleum hydrocarbon degrading function is obtained. According to the invention, the method is simple in construction, short in time, low in cost, large in yield rate and high in oil extraction productivity.

Description

technical field [0001] The invention belongs to the technical field of microbial oil recovery, and in particular relates to a method for increasing crude oil yield and recovery by utilizing the synergistic action of a combined bacterial solution containing phosphorus-solubilizing and nitrogen-fixing bacteria. Background technique [0002] As a non-renewable resource, oil determines that it is an indispensable and important strategic resource to ensure national security, the sustainable development of the national economy and the continuous improvement of people's living standards. With the rapid development of economy and modern society, the world's oil consumption has increased sharply, international oil prices have continued to rise, and the contradiction between supply and demand of oil products has become increasingly tense. Therefore, the shortage of oil resources is the most severe test and reality that must be faced by future human beings. [0003] my country is a bi...

AI Technical Summary

Problems solved by technology

Microbes in the formation generally need nitrogen, phosphorus compounds, oxygen-containing compounds, carbon-containing compounds, sulfur, various trace elements, hydrogen, vitamins, carbon dioxide, etc., especially to have sufficient nitrogen and phosphorus nutrients to ensure the high metabolism of microorganisms activity, but the nitrogen and phosphorus nutrients in the reservoir formation water are very few, so the content of nitrogen and phosphorus becomes the limiting factor restricting their massive growth and oil displacement. Therefore, the microorganisms injected into the formation must provide nitrogen and phosphorus However, the injected nutrients are continuously consumed by microorganisms and diluted by the injected water, the concentration of nutrients decreases, the retention and metabolism time in the reservoir is short, and the interaction time with the rocks and fluids in the oil reservoir is not enough, so the microbial flooding cannot be fully exerted. Therefore, it is necessary to develop and develop a method for the continuous supply of nitrogen and phosphorus nutrients, so as to greatly improve the oil displacement effect