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Efficient grease degrading bacterium and application thereof

A high-efficiency grease and bacteria-degrading technology, applied in the direction of dissolution, bacteria, biochemical equipment and methods, etc., can solve the problems of increasing production costs, hindering effective separation, complex extraction and separation of biosurfactants, etc., to achieve good oil drainage activity, The effect of good emulsifying properties

Active Publication Date: 2014-01-22
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with synthetic surfactants, biosurfactants still have a relatively large disadvantage. The first point is that the extraction and separation of biosurfactants is more complicated, which increases production costs.
However, the low concentration and amphipathic nature of biosurfactants in the fermentation broth often prevent their effective separation

Method used

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  • Efficient grease degrading bacterium and application thereof
  • Efficient grease degrading bacterium and application thereof
  • Efficient grease degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Strain enrichment acclimation and isolation culture

[0035]Collect 5-10g of sludge samples from the grease treatment tank of a food processing factory in Yantai City, Shandong, put them into a 150-300mL triangular flask filled with 50-100mL sterile water and sterile glass beads, place on a shaker, 150-180r / Shake fully for 10-15min, take 5-8mL of the mixed solution, add 35-50mL of sterile enrichment culture solution (NaCl0.5, peptone 5.0, beef extract 0.5, peanut oil 4mL, pH7.2-7.4, dilute with water to 1000mL), cultured at 28-30°C, 150-250r / min. Observe the degradation and dissipation of oil every 24 hours, and add an appropriate amount of peanut oil to 4ml. Transfer 5-10mL culture to 30-50mL new medium every 4-6d to promote the growth of lipolytic bacteria. This acclimatization process lasts for 2 months.

[0036] To prepare an oil-selective plate, take 10 g of tryptone, 5 g of yeast extract, 10 g of NaCl, 12 g of agar powder, 10 g of peanut oil, 1 mL o...

Embodiment 2

[0037] Embodiment 2: analysis of oil degradation activity

[0038] After the UC13 strain was activated, it was inoculated in a medium with peanut oil as the only carbon source, and the medium components were peanut oil 10 mg / mL, NH 4 NO 3 0.2g / L, K 2 HPO 4 0.5g / L,KH 2 PO 4 0.5g / L, MgSO 4 .7H 2 O0.1g / L; fermented and cultivated in a constant temperature shaking incubator, the culture temperature is 28-30 ℃, the rotation speed is 150-250r / min; respectively take and cultivate until the 0th, 6th, 12th, 24th, 30th, 36th, 42th, 48h of bacteria liquid. Measure the absorbance value of each of the above-mentioned bacterial liquids at 600nm to determine the growth of the bacteria; meanwhile, take 5 mL of the fermentation liquids with different fermentation times, extract twice with an equal volume of n-hexane, and combine the organic phases. The combined organic phases were dehydrated and dried with anhydrous sodium sulfate, placed in a round-bottomed flask, and filtered under r...

Embodiment 3

[0039] Example 3: Degradability of UC13 to different oils

[0040] After the UC13 strain was activated, they were respectively inoculated in culture media with peanut oil, soybean oil, sesame oil, lard oil, and diesel oil as the only carbon source. The culture medium has the same composition as that in Example 2 except that the types of oils and fats are different. Fermentation and cultivation of the above-mentioned fermentation liquids in a constant temperature shaking incubator, the cultivation temperature is 28-30° C., and the rotation speed is 150-250 r / min; the bacterial liquid is cultivated to the 24th hour. Measure the absorbance at 600nm to determine the growth of bacteria; take 5mL of fermentation broth and extract with n-hexane. Calculate the degradation rate according to the method of Example 2. The degradation of UC13 to the different oils and fats tested is as follows: figure 2 shown. UC13 has the ability to degrade all the tested oils. The degradation abili...

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Abstract

The invention belongs to the production field of microbial fermentation, and in particular relates to an efficient grease degrading bacterium and an application thereof. The efficient grease degrading bacterium is of acinetobacter UC13, wherein the acinetobacter UC13 is preserved in CGMCC (China General Microbiological Culture Collection Center) on June 24th, 2013; the preservation number of the acinetobacter UC13 is CGMCC No:7818. The invention further relates to applications of the efficient grease degrading bacterium in treatment of kitchen wastes or petroleum hydrocarbon contaminants containing greases and in industrial refining relevant to the greases. A biological surfactant is a fermentation product obtained by fermenting the acinetobacter UC13. The biological surfactant is used for eliminating industrial greases and contaminants in the kitchen wastes containing the greases. The invention provides an extraction method of the biological surfactant. The extraction method is simple to operate and can be used for ensuring the high activity of the biological surfactant.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production, and more specifically relates to a high-efficiency oil-degrading bacteria and its application. Background technique [0002] There are three main methods for treating oil-polluted wastewater: physical, chemical, and biological. Physical and chemical methods such as salting out, electrolysis, and membrane separation have many disadvantages, such as large investment, large area, and special equipment. Simultaneously this kind of method can't effectively deal with the grease waste, also exists the danger of producing secondary pollution. In contrast, the biological treatment method is to use organisms, mainly microorganisms, to use oil as the carbon source and energy required for microbial growth, to transfer and transform oil, and to hydrolyze it into glycerol and fatty acids under the catalysis of enzymes, and finally degrade it. for H 2 O.CO 2 Wait. Compared with physical and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A62D3/02B01F17/00C12R1/01C09K23/00
Inventor 焦绪栋秦松
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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