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Preparation method, application and medicine composition and preparation of specific egg yolk immunoglobulin (IgY) and acinetobacter baumannii, as well as preparation and kit

A technology of egg yolk immunoglobulin and Acinetobacter baumannii, which is applied in the direction of antibacterial immunoglobulin, egg immunoglobulin, antibacterial drugs, etc., can solve the problems that have not been reported, and achieve the suppression of toxic effects and broad The effect of applying the foreground

Inactive Publication Date: 2013-03-20
DALIAN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of specific IgY in the prevention and treatment of human diseases includes infant rotavirus diarrhea, Helicobacter pylori infection, dental caries, etc., but the application in the prevention and treatment of Acinetobacter baumannii infectious diseases has not been reported yet

Method used

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  • Preparation method, application and medicine composition and preparation of specific egg yolk immunoglobulin (IgY) and acinetobacter baumannii, as well as preparation and kit
  • Preparation method, application and medicine composition and preparation of specific egg yolk immunoglobulin (IgY) and acinetobacter baumannii, as well as preparation and kit
  • Preparation method, application and medicine composition and preparation of specific egg yolk immunoglobulin (IgY) and acinetobacter baumannii, as well as preparation and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 (M group specific IgY)

[0031] (1) Preparation of antigen solution

[0032] Acinetobacter baumannii standard bacteria (ATCC-BAA1605, purchased from the American type culture collection, distributed by Dalian Chenyu Biochemical Reagent Co., Ltd.) (referred to as M bacteria) was inoculated in TSB liquid medium, 37 After culturing at ℃ for 20 hours, centrifuge at 6000rpm for 15min to collect the bacteria, and dilute the bacteria with normal saline to 10 9 cfu / ml, blood cell count. Then add 0.5% formaldehyde in the volume of the bacterial liquid to the bacterial liquid, inactivate at 37°C for 24 hours, and shake it from time to time. After passing the sterility test, an equal volume of Freund's complete adjuvant or Freund's incomplete adjuvant was added, fully emulsified to make a whole-bacteria inactivated vaccine, and stored at 4°C. If the vaccine is stratified before injection, it needs to be mixed thoroughly before use.

[0033] (2) Immune hens

[0034] ...

Embodiment 2

[0037] Example 2 (group Q specific IgY)

[0038] The multidrug-resistant Acinetobacter baumannii (referred to as Q bacteria) isolated from the hospital was treated in the same way to prepare the antigen solution. The other steps were the same as in Example 1 to prepare yolk immunoglobulin freeze-dried powder.

Embodiment 3

[0039] Example 3 (non-specific IgY)

[0040] Except for replacing the bacterial solution with physiological saline, the same method as in Example 1 was used to prepare yolk immunoglobulin freeze-dried powder.

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PUM

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Abstract

The invention provides a preparation method, application and medicine composition and preparation of specific egg yolk immunoglobulin (IgY) and acinetobacter baumannii, as well as a preparation and a kit. The preparation method is characterized by comprising the following steps of: preparing antigen liquor, immunizing hens, and separating and purifying the IgY. In-vivo and in-vitro experiments prove that the prepared specificity IgY has specific binding activity to the acinetobacter baumannii, can be used for effectively inhibiting the growth of the acinetobacter baumannii and alleviating a toxic effect generated by the acinetobacter baumannii, can be applied to prevention, treatment and diagnostic analysis on infectious diseases and complications of the acinetobacter baumannii, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a preparation method, application, pharmaceutical composition, preparation and kit of Acinetobacter baumannii-specific yolk immunoglobulin. Background technique [0002] Acinetobacter baumannii (Acinetobacter baumannii) is a non-fermenting gram-negative bacillus, which is the main bacterial group in Acinetobacter that causes human infection, mainly causing respiratory tract infection, bacteremia, urinary system infection, wound infection, meningitis, etc. It can also cause peritonitis, endocarditis, endophthalmitis, etc. The most common infection site is the lung, which is an important pathogen of hospital-acquired pneumonia. [0003] With the extensive use of clinical antibacterial drugs, the drug resistance of Acinetobacter baumannii has become stronger and stronger, and it has developed multi-drug resistance to commonly used antibacterial drugs such as cephalos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/02A61K39/40A61P31/04G01N33/569
Inventor 甄宇红龙亚一侯源源孙丽丹廖瀚丰雪舒晓宏孙永韩文琦赵亚丽
Owner DALIAN MEDICAL UNIVERSITY
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