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Microbial inoculum for bioremediating coastline polluted by oil spilling and preparation method thereof

A bioremediation and coastline technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as insufficient research, and achieve the effects of stable flora structure, accelerated degradation rate, and rapid growth

Active Publication Date: 2010-06-02
CHINA NAT OFFSHORE OIL CORP +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the existing research results at home and abroad, although the role of mixed bacterial culture in biodegradation has been fully affirmed, and some results have been applied in practice, the research on the synergistic mechanism of mixed bacterial culture is not deep enough.

Method used

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  • Microbial inoculum for bioremediating coastline polluted by oil spilling and preparation method thereof
  • Microbial inoculum for bioremediating coastline polluted by oil spilling and preparation method thereof
  • Microbial inoculum for bioremediating coastline polluted by oil spilling and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Bacteria for bioremediation of oil-spilled shorelines, consisting of the following bacteria: Bacillus pumilus CGMCC No.3384, Acinetobacter veniceus CGMCC No.3385, Pseudomonas mendoza CGMCC No.3386 and Ralston Peeteri Bacteria CGMCCNo.3387.

Embodiment 2

[0045] Bacteria used for bioremediation of oil-spilled coastlines consist of the following bacteria: Bacillus pumilus CGMCC No.3384, Acinetobacter veniceus CGMCC No.3385 and Pseudomonas mendoza CGMCC No.3386.

Embodiment 3

[0047] Bacteria used for bioremediation of oil-spilled coastlines consist of the following bacteria: Bacillus pumilus CGMCC No.3384, Acinetobacter venetica CGMCC No.3385 and Ralstonia piterii CGMCC No.3387.

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Abstract

The invention discloses a microbial inoculum for bioremediating a coastline polluted by oil spilling and a preparation method thereof. The microbial inoculum for bioremediating the coastline polluted by oil spilling is made of mixed fermentation liquor and a fixed carrier as raw materials, wherein the mixed fermentation liquor comprises bacillus pumilus CGMCCnO.3384 fermentation liquor, Venice acinetobacter CGMCCnO.3385 fermentation liquor and / or Doxa pseudomonad CGMCCnO.3386 fermentation liquor and / or Ralstonia pickettii CGMCCnO.3387 fermentation liquor. By using the synergic action of different strains on the degradation of various petroleum hydrocarbons, the microbial inoculum improves the degradation speed of petroleum hydrocarbon pollutants and thoroughly degrades various petroleum hydrocarbon pollutants; in addition, a flora formed by a plurality of strains of bacteria has more stable structure and can better adapt to various petroleum-polluted environments. The microbial inoculum rapidly grows on the polluted areas, forms competitive advantages and remarkably increases the quantity of hydrocarbon degraded microbes in the polluted areas, thereby improving the biodegradation rate of crude oil.

Description

technical field [0001] The invention belongs to the technical field of bioremediation of coastlines polluted by petroleum and petroleum products, and in particular relates to a bacterial agent for bioremediation of coastlines polluted by oil spills and a preparation method thereof. Background technique [0002] With the world's increasing demand for oil and its products, oil spill accidents in the process of offshore exploitation, transportation, loading and unloading, and utilization of oil are also increasing. Oil spills not only cause serious environmental pollution, but also lead to a serious decline in the environmental quality and biodiversity index of coastal waters due to the potential toxicity and bioaccumulation effects of petroleum hydrocarbon pollutants, destroying the function of the ecosystem, and affecting the aquatic industry and tourism. It will also cause huge economic losses. In addition, polycyclic aromatic hydrocarbons in petroleum hydrocarbons have str...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N11/14C12N11/10A62D3/02C12R1/08C12R1/01C12R1/38A62D101/20
Inventor 朱生凤陈宇杨仕美梁生康吴亮何云馨孙会霞李辉高祁
Owner CHINA NAT OFFSHORE OIL CORP
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