Efficient denitrification novel Acinetobacter and application thereof

An Acinetobacter, high-efficiency technology, applied in the field of microorganisms, can solve problems such as nitrite ammonia nitrogen exceeding the standard, and achieve the effect of protecting the environment and improving the ability of denitrification

Active Publication Date: 2016-02-17
GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a new species of Acinetobacter bacterium with denitrification function, which has the potential to be developed as a denitrification microbial bacterial agent, and is useful for solving eutrophication problems such as excessive nitrite ammonia nitrogen in aquaculture water , has a good application prospect

Method used

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  • Efficient denitrification novel Acinetobacter and application thereof
  • Efficient denitrification novel Acinetobacter and application thereof
  • Efficient denitrification novel Acinetobacter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The acquisition of embodiment 1 Acinetobacter new bacteria

[0018] 1.1 Prepare the following medium:

[0019] Ammonia oxidation medium: NH 4 Cl0.382g, sodium acetate 2g, MgSO 4 7H 2 O0.05g, K 2 HPO 4 0.2g, NaCl0.12g, MnSO 4 4H 2 O0.01g, FeSO 4 0.01g, dilute water to 1000mL, pH7.2;

[0020] Nitrite reducing medium: NaNO 2 0.04928g, glucose 10g, sodium acetate 1g, Na 2 HPO 4 3g, KH 2 PO 4 2g, NaCl3g, water 1L, pH7.2; add 1.5% agar to the solid medium.

[0021] 1.2 Screening of heterotrophic nitrifying bacteria

[0022] 1. Collect samples : The samples in the experiment were collected from the pond bottom mud in Nansha District, Guangzhou City, Guangdong Province.

[0023] 2. Microbial enrichment culture:

[0024] (1) Take 20mL of the collected fresh sample and add it to 180mL of ammonia oxidation medium. After enrichment culture at 30°C and 180r / min for 3 days, repeat the enrichment once to obtain the enrichment solution, which is set aside.

...

Embodiment 2

[0028] Embodiment 2: the characteristic of novel microorganism of the present invention

[0029] 2.1 Identification of strain morphological characteristics

[0030] Take a single colony of strain YZS-X1-1, transfer it to LB solid medium (agar), and culture it in a constant temperature incubator at 30°C for 24h, 36h, and 48h, and observe the size, color, edge, and protrusion of the colony respectively , smoothness, viscosity, transparency and other characteristics. The results showed that strain YZS-X1-1 formed neat, smooth, viscous, glossy and opaque colonies on LB solid medium. Observed under the microscope, it is short rod-shaped, and the result is as follows figure 1 shown.

[0031] 2.2 Physiological and biochemical characteristics of the strain

[0032] Pick fresh cultures cultured on LB solid medium (agar) for 24 hours for physiological and biochemical tests. The results showed that YZS-X1-1 was a Gram-negative bacillus.

[0033] 1. Identification characteris...

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Abstract

The invention discloses an efficient denitrification novel Acinetobacter and application thereof. Acinetobacter guangzhouensis YZS-X1-1 is collected in China Center for Type Culture Collection on 6th August, 2014 under CCTCC NO. M2014369. The Acinetobacter has good denitrification capacity and is effective in removing ammonia nitrogen and nitrite nitrogen to protect the environment. The Acinetobacter can also be made into biological denitrification bacterial agents.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to an Acinetobacter ( Acinetobacter ) is a new species of bacteria. Background technique [0002] Nitrogen is an important pollutant in water pollution. It mainly exists in two forms of organic nitrogen and inorganic nitrogen. Organic nitrogen includes proteins, amino acids, urea, etc.; inorganic nitrogen mainly includes ammonia nitrogen, nitrate nitrogen and nitrite nitrogen. In recent years, with the use of nitrogen fertilizer and the development of high-density aquaculture, although it has brought huge economic benefits, it has also caused serious environmental problems. [0003] Pond aquaculture occupies an important position in my country's aquaculture system. In 2009, the area of ​​freshwater pond aquaculture in my country reached 42.99% of the total area of ​​freshwater aquaculture. At the same time, the aquaculture pond system is also a special type of water system...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 张宏刚张文艳刘陈立
Owner GUANGZHOU INST OF ADVANCED TECH CHINESE ACAD OF SCI
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