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Heterotrophic nitrification aerobic denitrifying bacteria, culturing method thereof and uses

An aerobic denitrification and heterotrophic nitrification technology is applied in the field of efficient heterotrophic nitrification aerobic denitrification bacteria and their cultivation, and can solve the problems of increased energy consumption, large energy consumption, oxygen consumption and the like

Active Publication Date: 2009-01-07
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The process is lengthy, because the process conditions of nitrification and denitrification are different, they need to be completed in two systems respectively, resulting in two deficiencies
First of all, it consumes a lot of energy. Ammonia nitrogen nitrification requires oxygen consumption, that is, it needs to consume energy to supply oxygen. The pre-denitrification system needs to set a relatively large reflux in the mixed liquid, which also increases energy consumption.
Secondly, the denitrification reaction must have a carbon source as an electron donor. If the carbon source in the sewage is insufficient (C / N is too low), then organic carbon such as methanol needs to be added, which not only increases the operating cost, but also increases the operational management and maintenance costs. The difficulty of subsequent processing
Therefore, the investment in wastewater treatment projects is large and the operating costs are high.
Moreover, nitrifying bacteria are usually autotrophic nitrifying bacteria, which proliferate slowly and are easily eliminated in the wastewater biological treatment system, thus affecting the stability of the denitrification effect of the wastewater biological treatment system

Method used

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  • Heterotrophic nitrification aerobic denitrifying bacteria, culturing method thereof and uses
  • Heterotrophic nitrification aerobic denitrifying bacteria, culturing method thereof and uses
  • Heterotrophic nitrification aerobic denitrifying bacteria, culturing method thereof and uses

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Embodiment 1

[0098] Embodiment 1: the cultivation of bacterial strain of the present invention

[0099] (1) Beef extract medium and organic carbon source denitrification medium, sterilized at 121°C for 20 minutes.

[0100] (2) The strain Pseudomonas putida DN1.2 was inoculated on the slope of sterilized beef extract, activated and cultivated at 35°C for 10-16 hours for later use.

[0101] (3) Inoculate the activated strains in beef extract liquid medium or organic carbon source denitrification liquid medium respectively, fill 50mL medium in a 250mL Erlenmeyer flask, and culture at 35°C, 170-200rpm, for 10-16h with shaking. The growth curve of DN1.2 in the beef extract medium is attached image 3 . It can be seen from the figure that DN1.2 grows very well in the beef extract medium, and the lag period is very short. It is in the logarithmic growth phase within 3 to 10 hours, and it can reach the stable phase after 10 hours.

[0102] (4) 4000rpm, 10min centrifugation to harvest the bacter...

Embodiment 2

[0103] Embodiment 2: Denitrification of bacterial strains of the present invention under inorganic carbon source conditions

[0104] Autotrophic nitrification medium (g / L): (NH 4 ) 2 SO 4 2,K 2 HPO 4 1. MgSO 4 ·7H 2 O 0.5, FeSO 4 ·7H 2 O0.4, NaCl2. After bottling, add CaCO in a ratio of 0.5% by mass 3 , and finally adjust the pH to 7.2 with 1% NaOH solution, and sterilize at 121°C for 20 minutes.

[0105] Use autotrophic nitrification medium, inoculate 10% bacterial suspension (cultivation method sees embodiment 1), control replaces with the same volume of sterilized water, 30 ℃, 170rpm vibration culture 10 hours, regular sampling measures ammonia nitrogen, nitrate nitrogen, Changes in the concentration of nitrite nitrogen. The experimental results are shown in Table 2. It can be seen from Table 2 that in the inorganic carbon source denitrification medium with an initial ammonia nitrogen concentration of 56.6 mg / L, after 10 hours of shaking culture, DN1.2 reduced...

Embodiment 3

[0108] Embodiment 3: Denitrification of bacterial strains of the present invention under organic carbon source conditions

[0109] Organic carbon source denitrification medium (g / L): KH 2 PO 4 0.7, MgSO 4 ·7H 2 O 0.5, CaCl 2 2H 2 O 0.5, (NH 4 ) 2 SO 4 0.5, glucose 1.0-4.0, NaOH to adjust the pH to 7.2-7.4, and sterilize at 121°C for 20 minutes.

[0110] (1) Removal of ammonia nitrogen under the condition of organic carbon source

[0111] Use organic carbon source denitrification medium (prepared COD Cr About 1000mg / L, about 80mg / L of ammonia nitrogen), inoculate 6.7% of the bacterial suspension (see Example 1 for the culture method), replace with the same volume of sterilized water as the control, 30°C, 170rpm shaking culture for 12 hours, regular sampling and determination Changes in the concentrations of ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen. The results are shown in Table 3. It can be seen from Table 3 that DN1.2 can also remove ammonia nitr...

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Abstract

The invention belongs to the environmental microorganism field, relates to a high efficiency heterotrophic nitration aerobic denitrifying bacteria and the culture method and the purpose. The bacterium is Pseudomonas putida DN1.2 with the preservation and registration number of CCMCC M207075, which can effectively remove the ammonia nitrogen, nitrous acid and the mixture in water. At the same time, the bacterium also can remove the CODCr in the organic waste water. The bacterium is suitable for being used in the treatment of organic nitrogenous effluent with high concentration and inorganic nitrogenous effluent. The nitrite and nitrate can not be produced in the denitrification process. The waste water treatment process is simple by using the strain and the denitrification effect is stable.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a high-efficiency heterotrophic nitrification-aerobic denitrification bacterium and its cultivation method and application. Background technique [0002] Nitrogen-containing wastewater is a major problem in environmental pollution control. Biological denitrification is currently recognized as one of the most economical and effective methods for denitrification of wastewater. The traditional ammonia nitrogen wastewater treatment is to convert ammonia nitrogen into nitrogen gas through the combined process of nitrification by autotrophic nitrifying bacteria and denitrification by heterotrophic denitrifying bacteria. [0003] [0004] The process is tedious, and due to the different process conditions of nitrification and denitrification, it needs to be completed in two systems respectively, resulting in two deficiencies. First of all, it consumes a lot o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/40
Inventor 黄钧杨航李毅军
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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