11 results about "Lactococcus garvieae" patented technology

The invention discloses a compound probiotic preparation for breeding macrobrachium rosenbergii as well as a preparation method and application of the compound probiotic preparation, and relates to the technical field of microorganisms, the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski, and the compound probiotic preparation comprises the following active strains: Lactococcus gasseri, Bacillus subtilis and Nitrobacter winogradski. Wherein the preservation number of the lactococcus format is CGMCC (China General Microbiological Culture Collection Center) No.31024. According to the physiological requirements of different development stages of the Z1-Z4 stage and the Z5-P1 stage of larvae, 2: 1 and 2: 1: 1 bacterial strains are adopted for staged accurate addition respectively, meanwhile, artificial seawater of a specific formula is matched, baits such as fairy shrimps and egg custard are quantitatively fed according to the development stage, key water quality indexes such as dissolved oxygen, ammonia nitrogen and nitrite in a water body are strictly regulated and controlled, and the quality of the larvae is improved. A green seedling raising technical system integrating probiotic micro-ecological regulation and control, precise nutrition supply and water quality management is formed, and finally the effects of improving the survival rate of larvae, reducing the metamorphosis rate, promoting growth and development and replacing antibiotics for use are achieved.

The lactococcus gasseri is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37371, the preservation date is January 13, 2026, the lactococcus gasseri is separated from Guangdong buffalo raw milk and is obtained by directional screening of a butter system, and the lactococcus gasseri is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.37371, the preservation number is CGMCC No.37371, and the preservation date is January 13, 2026. The strain is gram-positive hammer-shaped, and has excellent colonial morphology and high biological safety. The invention also discloses a method for preparing fermented butter by using the strain, a leavening agent is prepared through activation, culture, centrifugation and resuspension, then the butter is fermented, and an optimal fermentation process is determined through a single factor experiment and response surface optimization. The content of 2, 3-butanedione in the prepared fermented butter is larger than or equal to 5000 micrograms / kg, the relative content of conjugated linoleic acid is larger than or equal to 0.70%, the acid value is smaller than or equal to 1.35 mg / g, the peroxide value is smaller than or equal to 1.49 mmol / kg, and the fermented butter is rich in flavor and excellent in quality. The fermented butter can be applied to preparation of foods such as cookies and smearing sauce, and the texture, color and flavor of the foods can be remarkably improved.

This invention relates to the field of microbial directed fermentation processing technology for yak milk fat products, and discloses a compound microbial agent for yak butter fermentation and its application. The compound microbial agent includes *Lactococcus gasseri* (…). Lactococcus garvieae CGMCC No. 32756, Enterococcus faecium ( Enterococcus faecium CGMCC NO.36378 and Kluyveromyces martensii ( Kluyveromyces Marxian (CGMCC NO.39019) All three strains exist in live form. This invention also discloses a method for preparing the composite microbial agent, a method for fermenting yak ghee using the composite microbial agent, and the resulting fermented yak ghee. The three strains possess environmental tolerance and single-strain growth characteristics suitable for optimizing the synergistic fermentation process of yak ghee; the interaction of the three strains can enhance the growth performance of the strains. Inoculating the composite microbial agent into yak milk fat substrate for fermentation can improve the flavor of yak ghee, reduce acid value, peroxide value, and biogenic amine accumulation, and improve the controllability of the yak ghee fermentation process and the stability of product quality.

This invention discloses a strain of *Lactococcus gasseri* LG3092 that produces the bacteriocin GarQ and effectively antagonizes multidrug-resistant *Helicobacter pylori*, and its applications. *Lactococcus gasseri* LG3092 was deposited at the Guangdong Provincial Microbial Culture Collection Center on October 30, 2023, with accession number GDMCC No: 63939. Studies have shown that LG3092 has the following beneficial effects: (1) it can produce the bacteriocin GarQ, effectively inhibiting the growth of *Helicobacter pylori* and other common pathogenic bacteria; (2) it has a high survival rate in the stomach, can colonize the stomach, and can compete with pathogenic bacteria for binding sites, effectively reducing the infection rate of *Helicobacter pylori* in the stomach; (3) it can improve the pathological state of the stomach and alleviate inflammation. Therefore, *Lactococcus gasseri* LG3092 has great application potential in the preparation of products for the prevention or treatment of *H. pylori*, especially multidrug-resistant *H. pylori* infection.

The invention discloses application of lactococcus gasseri ZB15 in degradation of kitasamycin, and belongs to the technical field of microorganism application. The invention discovers and verifies that the lactococcus gasseri ZB15 strain has the capability of efficiently degrading kitasamycin for the first time. Specific experimental data show that the strain can maintain growth in a culture medium containing kitasamycin with a gradient concentration of 0-6 [mu] g / mL, and shows good tolerance. High performance liquid chromatography analysis proves that in a set culture period, the removal effect of the strain on kitasamycin mainly depends on a biodegradation effect instead of a pure adsorption effect. Furthermore, the influence of key parameters such as inoculum size, initial concentration, temperature and pH on the degradation efficiency is systematically researched, and the degradation characteristic and the optimal working window of the kitasamycin degrading bacteria are completely analyzed, so that a microbial resource with definite performance is obtained, and the blank of lacking of safe and efficient kitasamycin degrading bacteria in the prior art is filled.

The present application relates to the fermentation technical field, especially to a lactococcus garvieae ZB15 and cell-free fermentation freeze-dried material with anti-inflammatory activity and a preparation method thereof.The lactococcus garvieae ZB15 is preserved in Guangdong Microbial Culture Collection Center, the address is No. 59, Building 5, Institute of Microbiology, Guangdong Academy of Sciences, 100, Martyrs' Road, Guangzhou, the preservation date is February 27, 2025, and the preservation number is GDMCC No: 65947.The lactococcus garvieae ZB15 of the present application is prepared into cell-free fermentation freeze-dried material, the cell-free fermentation freeze-dried material can relieve and improve enteritis, improve the immune capacity of the body by changing the intestinal microbial community structure, and provide a basis for the preparation of enteritis drugs.

The invention discloses an indoor cultivation method for increasing the survival rate of larvae and juveniles of horse mackerels, and relates to the technical field of aquaculture. Pretreated rotifers, shadberry fruit extracts and 2, 4 '-dihydroxychalcone are added in the early larva fish breeding stage and the later larva fish breeding stage, active ingredients such as gulcomannan, proline, chlorogenic acid and potentilla discolor extracts are added in the juvenile fish breeding stage, and meanwhile environmental factors such as illumination and sound wave stimulation are regulated and controlled, so that the juvenile fish breeding efficiency is improved. The survival rate of the horse mackerels and the capability of resisting infection of lactococcus gasseri and vibrio anguillarum can be effectively improved, the investment of the breeding cost is reduced, and the used baits are simple, easy to obtain and simple to prepare.

The present application relates to antibacterial peptide TroLEAP2-21 and its application in the preparation of antibacterial and maintaining fish tissue integrity products, belong to the technical field of biological polypeptide, the amino acid sequence of the antibacterial peptide is shown as SEQ ID NO.1.The present application also provides the application of the antibacterial peptide TroLEAP2-21 of the Trachurus japonicus in the preparation of antibacterial products, the bacteria are Lactococcus garviae and Staphylococcus epidermidis.The antibacterial peptide TroLEAP2-21 can change the permeability of bacterial cell membrane, induce the depolarization of bacterial cell membrane, degrade bacterial genomic DNA, and cause the morphological change of bacteria, thereby playing an antibacterial role.

This invention discloses a triplet real-time PCR detection method for Streptococcus agalactiae, Streptococcus dolphinus, and Lactococcus gasseri. It utilizes three primer sets and probes: one for Streptococcus agalactiae, one for Streptococcus dolphinus, and one for Lactococcus gasseri. By optimizing the degradation temperature, primer dosage, and probe dosage, this invention effectively reduces the mutual interference of the three target pathogens, improving the accuracy of the detection results. This detection method has significant application value in fish farming. The reagent kit of this invention has low preparation cost, a simple preparation process, and is easily scalable for industrial production, thus possessing broad market prospects.

The invention discloses a compound microbial fermentation preparation for silage of urtica cannabina and application of the compound microbial fermentation preparation, and belongs to the technical field of microbial fermentation products, the microbial fermentation preparation is prepared from the following raw materials in parts by weight: 0.5-1.5 parts of lactococcus gasseri OL454842, 0.5-1.5 parts of lactobacillus plantarum, 0.3-1.7 parts of molasses and 0.5-1.5 parts of urea. The microbial fermentation preparation is applied to urtica cannabina silage, the success rate of urtica cannabina silage fermentation can be effectively increased, the pH value of urtica cannabina silage is reduced, the fermentation quality of urtica cannabina is improved, and help is provided for solving the problem of forage grass shortage in winter and assisting animal husbandry development.

The invention discloses a Lactococcus gasseri HN1 strain and an application of the Lactococcus gasseri HN1 strain. The Lactococcus garviae HN1 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 67511. The HN1 strain can resist acid and bile salt and is good in safety, supernate of the HN1 strain has an obvious bacteriostatic effect on aquatic / zoonosis pathogenic bacteria such as vibrio harveyi, escherichia coli, vibrio anguillarum, aeromonas hydrophila, staphylococcus aureus, vibrio parahaemolyticus and edwardsiella tarda, and the HN1 strain can be prepared into antibacterial drugs and used for preventing and treating related diseases. Besides, the HN1 strain is very sensitive to ampicillin and tetracycline, is insensitive to antibacterial drugs such as rifampicin and the like, and also can be combined with insensitive or drug-resistant antibacterial drugs to better prevent and treat diseases caused by pathogenic bacterium infection.