43 results about "Lactococcus garvieae" patented technology

The invention is in the field of molecular biology, and relates to recombinant engineering and protein expression. More in particular, the invention relates to nucleic acids for recombinant expression of proteins comprising sequences derived from Lactococcus and useful as promoters. The invention further relates to vectors comprising the said nucleic acids and host cells transformed therewith. The invention also covers the use of host cells comprising the said nucleic acids or vectors for expressing heterologous or homologous proteins; and also for delivery, especially therapeutic delivery, of the said proteins to subjects.

A complete nutritional composition comprising Lactococcus strains or probiotic is provided for reducing the symptoms of allergies in different groups of patients such as allergies originating from food allergens in young children or infants and respiratory allergens in children, adults and household pets. Preferably the composition reduces symptoms of allergies (secondary prevention) while not significantly affecting sensitization (primary prevention). The composition comprises a probiotic of the genus Lactococcus.

The invention relates to the technical field of feed additive and processing thereof, and in particular to a probiotic feed additive. The probiotic feed additive is prepared by mixing autonomously separated lactobacillus plantarum, candida rugosa, candida ethanolica and lactococcus garvieae and buffalo milk fermentation substrate to ferment. The preparation process disclosed by the invention is simple, and the probiotic feed additive can be widely applied in the raising of livestock and poultry such as milk buffalos, pigs, chickens, cattle and sheep. The probiotic feed additive can remarkably improve the intestinal microbial floras of animals and enhance immunity, and can reduce the usage of antibiotic medicines and eliminate medicine residue, and when applied in production, the probiotic feed additive can shorten feeding periods and save feeds, thus increasing farming income.

The invention discloses a construction method and application of a faeG expressing gene engineering probiotic. The method comprises the following steps of: according to the gene sequence and the expression vector plasmid characteristic of a disclosed F4 escherichia coli pilus adhesin faeG, designing a primer which contains a specific endonuclease site; performing polymerase chain reaction (PCR) amplification by taking deoxyribonucleic acid (DNA) of an enterotoxigenic escherichia coli plasmid as a template to obtain a faeG gene-containing segment, linking the segment with an expression vector plasmid pNZ8148 to obtain a recombinant plasmid pNZ8148-faeG, transferring the recombinant plasmid into lactobacillus or a galactococcus cell by using an electrotransformation method to obtain gene engineering probiotic which expresses enterotoxigenic escherichia coli pilus adhesin faeG, expressing under the induction of nisin and analyzing and proving a target gene by SDS-PAGE and Western blot so as to express correctly. A recombinant strain can be used for preventing colibacillus diarrhea of suckling pigs and weanling pigs.

The invention relates to a lactococcus garviea bacteriocin, a preparing method thereof and an application thereof. The high-yield actococcus garviea bacteriocin can be obtained in the mode that lactococcus garviea is subjected to fermented cultivation, and then the bacteriocin in the cultivating-fermenting liquid is extracted with a pH adsorption method; more than 800 mg of the lactococcus garviea bacteriocin can be prepared through 1 L of the fermenting liquid. The lactococcus garviea bacteriocin has quite high broad-spectrum bacteriostasis capacity and has a quite high inhibiting effect on some gram negative bacteria, some gram positive bacteria, yeast and mould causing human diseases and food spoilage. The lactococcus garviea bacteriocin has the effect of treating and preventing acute and chronic enteritis.

The present invention pertains to a lactic acid bacterium belonging to the genus Lactococcus and having a receipt number NITE ABP-01694 with the Patent Microorganisms Depositary (NPMD) of the National Institute of Technology and Evaluation (NITE), a natural immunostimulant having this lactic acid bacterium as an active ingredient, a food or drink containing this lactic acid bacterium or this natural immunostimulant, and yogurt containing lactic acid bacteria, wherein the yogurt has resistance to at least one bacterium selected from the group comprising methicillin-sensitive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus mundtii.

The invention discloses a compound biological fresh-keeping agent and the application thereof. The compound biological fresh-keeping agent is composed of lactococcus garvieae, helianthus tuberosus linn leaf alcohol extract and water-soluble chitosan. Lactococcus garvieae is prepared through fermentation and then extraction of lactococcus garvieae SY-1, and the strain is preserved in the China General Microbiological Culture Collection Center of China Microbe Preservation Management Committee with preservation number of CGMCC NO.9485 on July 28, 2014. The helianthus tuberosus linn leaf alcohol extract is prepared through ultrasonic extraction. The fresh-keeping agent is applied to fresh-keeping of small berries, a safe, poisonous and edible transparent film can be formed on the surface of fruit, and through conventional refrigerator preservation, the color and nutrients of fruit can be effectively maintained and the shelf life of various small berries can be prolonged remarkably.

The invention discloses an LAMP primer for detecting needle mushroom surface Lactococcus. The LAMP primer consists of nucleotide sequences shown by SEQ ID No. 1 to SEQ ID No. 6. The needle mushroom Lactococcus mainly refers to Lactococcus lactis subsp. hordniae, Lactococcus lactis subsp. Lactis or Lactococcus garvieae strains. The invention also discloses a detection kit containing the LAMP primer and a corresponding detection method. The LAMP primer provided by the invention has strong specificity and high sensitivity, and has the advantages of simple method, high detection speed, easy observation, and no need for expensive equipment and professionals.

The invention relates to the field of microbial technology, and relates to a strain of Lactococcus garvieae and a feed additive produced therefrom. The feed additive is a solid microbial inoculum prepared from a liquid microbial inoculum through addition of proper nutrient substances, wherein the liquid microbial inoculum is prepared from Lactococcus garvieae SYP-B-301 through fermentation culture in a fermentation cylinder. The feed additive has a wide range of applications in animal husbandry and poultry raising such as pig raising, chicken raising, cattle raising and the like. The feed additive has a plurality of functions, through use of the feed additive, animal immunity after eating can be improved, animal diarrhoea rate and death rate can be reduced, the use of antibiotic drugs can be reduced, drug residues can be eliminated, production efficiency can be obviously improved, the raising cycle can be shortened, and feed can be effectively saved, so that income of farmers can be increased.

The present invention is lactococcus for producing beta-galactosidase and the production process of beta-galactosidase with the lactococcus strain. By means of preservation, rejuvenation and breeding of the lactococcus strain (CGMCC. No.1425) and optimized fermentation process, the present invention has short fermentation period and stable fermentation process to obtain beta-galactosidase with excellent high temperature activity and high enzyme activity. The product may be used widely in fermented milk product, beverage, food, medicine and other fields.

The present invention relates to a 2227-bp DNA fragment that confers antiphage activity. Two proteins encoded by this DNA sequence are responsible for the phage resistance phenotype. When introduced in Lactococcus lactis phage-sensitive strain, this DNA fragment aborts infection of phages of the 936 and P335 species.

Lactococcus lactis strains with improved preservation characteristics, and improved acid and bile salt tolerance are disclosed. More specifically, a L. lactis strain having a heterologous trehalose-6-phosphate synthase gene and/or a trehalose-6-phosphate phosphatase gene, results in an accumulation of trehalose in the cytoplasm and/or in the cytoplasmic membrane. A L. lactis strain, having an internal trehalose concentration of at least 10 mg per gram cells (w/w) is disclosed.

The invention relates to gamma-decalactone prepared based on biotransformation and a preparation method thereof. The gamma-decalactone is prepared by the following steps: inoculating yarrowia lipolytica into a fermentation culture medium, transforming and inoculating the obtained fermentation liquor into a transformation culture medium, carrying out transformation culture, adding oleic acid and oleic acid hydratase in batches in the process, and continuously carrying out transformation culture. The gamma-decalactone is produced through a biological fermentation and conversion technology with the waste grease mixture as the substrate, the problem that in an existing biological conversion technology, the substrate is single is solved with the waste grease mixture as the substrate, the waste grease mixture is rich in source, waste utilization is achieved, and the method is economical and environmentally friendly; meanwhile, the lactococcus gasseri genome DNA is used as a template, the oleic acid hydratase is obtained through PCR amplification, the activity of the oleic acid hydratase is improved, and the production yield of the gamma-decalactone is increased.