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Lactococcus garviea bacteriocin, preparing method thereof and application thereof

A technology of Lactococcus gasseri and bacteriocin, applied in the field of microorganisms, can solve the problems of narrow application range, instability and ineffectiveness of lactic acid bacteria, achieve strong broad-spectrum bacteriostatic ability, and treat and prevent acute and chronic enteritis.

Active Publication Date: 2016-03-09
GUANGZHOU KANGYOUYUAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Only Nisin and pediocinPA-1 / AcH are currently used in industry, but because these bacteriocins only have inhibitory effects on most Gram-positive bacteria, but have no effect on Gram-negative bacteria, and are unstable in acidic environments , which makes the scope of application of lactic acid bacteria narrow, limiting its application in food

Method used

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  • Lactococcus garviea bacteriocin, preparing method thereof and application thereof
  • Lactococcus garviea bacteriocin, preparing method thereof and application thereof
  • Lactococcus garviea bacteriocin, preparing method thereof and application thereof

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Embodiment 1

[0029] A kind of preparation method of Lactococcus garviea (Lactococcus garviea) bacteriocin of the present embodiment, comprises the following steps:

[0030] (1) Activation of Lactococcus garviea: activate Lactococcus garviea in nutrient broth for 48 hours, the activation temperature is 32°C, and the rotation speed is 240rpm;

[0031] (2) Fermentation culture of Lactococcus gravidarum: the activated Lactococcus garviea (Lactococcus garviea) was inoculated in the improved M17 medium at an inoculum size of 10% (v / v), fermented and cultivated for 144 hours, and the culture temperature was 32°C. The rotating speed is 100rpm, and the fermented liquid is obtained; the composition of the improved M17 medium is: 0.1g of L-arabinose, 5g of plant peptone, 5g of yeast powder, 5g of polypeptone, 0.5g of ascorbic acid, 1.5g of beef extract, MgSO 4 ·7H 2 O0.01g, β-glycerophosphate disodium 10g, distilled water 1000mL;

[0032] (3) Extraction of bacteriocin: adjust the pH value of the fe...

Embodiment 2

[0034] A kind of preparation method of Lactococcus garviea (Lactococcus garviea) bacteriocin of the present embodiment, comprises the following steps:

[0035] (1) Activation of Lactococcus garviea: activate Lactococcus garviea in nutrient broth for 12 hours, the activation temperature is 37°C, and the rotation speed is 240rpm;

[0036](2) Fermentative culture of Lactococcus garrison: the activated Lactococcus garviea (Lactococcus garviea) was inoculated in the improved M17 medium at an inoculum size of 0.5% (v / v), fermented and cultivated for 12 hours, and the culture temperature was 37°C. The rotating speed is 100rpm, and the fermented liquid is obtained; the composition of the improved M17 medium is: L-arabinose 5g, plant peptone 10g, yeast powder 10g, polypeptone 5g, ascorbic acid 0.5g, beef extract 1.5g, MgSO 4 ·7H 2 O0.01g, β-glycerophosphate disodium 10g, distilled water 1000mL;

[0037] (3) Extraction of bacteriocin: adjust the pH value of the fermented liquid obtain...

Embodiment 3

[0039] A kind of preparation method of Lactococcus garviea (Lactococcus garviea) bacteriocin of the present embodiment, comprises the following steps:

[0040] (1) Activation of Lactococcus garviea: activate Lactococcus garviea in nutrient broth for 12 hours, the activation temperature is 37°C, and the rotation speed is 240rpm;

[0041] (2) Fermentative culture of Lactococcus garrison: the activated Lactococcus garviea (Lactococcus garviea) was inoculated in the improved M17 medium at an inoculum size of 0.5% (v / v), fermented and cultivated for 12 hours, and the culture temperature was 37°C. The rotating speed is 240rpm, and the fermented liquid is obtained; the composition of the improved M17 medium is: L-arabinose 1g, plant peptone 10g, yeast powder 10g, polypeptone 5g, ascorbic acid 0.5g, beef extract 1.5g, MgSO 4 ·7H 2 O0.01g, β-glycerophosphate disodium 10g, distilled water 1000mL;

[0042] (3) Extraction of bacteriocin: adjust the pH value of the fermented liquid obtai...

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Abstract

The invention relates to a lactococcus garviea bacteriocin, a preparing method thereof and an application thereof. The high-yield actococcus garviea bacteriocin can be obtained in the mode that lactococcus garviea is subjected to fermented cultivation, and then the bacteriocin in the cultivating-fermenting liquid is extracted with a pH adsorption method; more than 800 mg of the lactococcus garviea bacteriocin can be prepared through 1 L of the fermenting liquid. The lactococcus garviea bacteriocin has quite high broad-spectrum bacteriostasis capacity and has a quite high inhibiting effect on some gram negative bacteria, some gram positive bacteria, yeast and mould causing human diseases and food spoilage. The lactococcus garviea bacteriocin has the effect of treating and preventing acute and chronic enteritis.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactococcus gasseri bacteriocin and a preparation method and application thereof. Background technique [0002] Bacteriocins are a class of proteins or polypeptides with antibacterial activity that are secreted into the environment by some bacteria through the ribosome synthesis mechanism. The fundamental difference between bacteriocins and antibiotics is that most bacteriocins only damage closely related bacteria, and are non-toxic, have no side effects, no residue, no drug resistance, and do not pollute the environment. Therefore, bacteriocins can partially reduce or even replace the use of antibiotics. Bacteriocins are non-toxic, have no side effects, no residue, no drug resistance, and can inhibit or kill some food spoilage bacteria. They have certain thermal stability and are easily degraded by some proteases in the human digestive tract, so they will not accumulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00C07K14/315A61K38/16A61P1/00A01N63/02A01P1/00A01P3/00C12R1/46
CPCA01N63/10A61K38/00C07K14/315
Inventor 司朝利廖振林赖学能
Owner GUANGZHOU KANGYOUYUAN BIOTECH CO LTD
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