Method of preparing walnut polypeptide powder
A production method and walnut polypeptide technology are applied in the protein composition of vegetable seeds, medical preparations containing active ingredients, food preparation, etc., and can solve problems such as no similar technologies have been found.
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[0025] Protein extraction: When extracting protein, use ultrasonic extraction method, squeeze the walnut residue after oil, crush (through 40 mesh sieve), soak, adjust pH to 8.0-8.5, temperature 50-60°C, solid-liquid ratio 1: 15~1:25, use frequency of 20~50kHz ultrasonic leaching for 1h, then adjust the pH to 4.0~4.5 for protein acid precipitation, let it stand for 4~8h, remove the supernatant, and deposit the obtained precipitate at 3000~4000r / Centrifuge for 15-20 min, remove the supernatant, and obtain the protein.
[0026] Drying: When protein is processed, vacuum drying method is adopted, the temperature of the vacuum chamber is 60-70°C, the degree of vacuum is 0.05-0.07MPa, and the drying time is 8-10 hours.
[0027] Enzymolysis: When the dried walnut protein is enzymatically hydrolyzed, the protease used is papain, and the enzymolysis conditions are: enzyme dosage 4000-6000U / g, substrate concentration 2%-5%, enzymolysis temperature 55-50% 65°C, pH value 6.5-7.5, enzyma...
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