Method for rapidly extracting polysaccharide from lentinus edodes

A technology of lentinan and lentinus edodes, applied in the field of lentinus edodes, can solve problems such as poor operation continuity, long production cycle, and affecting output efficiency, and achieve the effects of reasonable process flow configuration, improved operating efficiency, and high degree of automation

Inactive Publication Date: 2013-07-17
RONGSHENGLONG SUIZHOU FOOD CO LTD
View PDF0 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problems existing in the prior art are mainly summarized as follows: (1) Excessive introduction of organic solvents; (2) Some methods have complicated manual operation procedures; (3) The cost of the refining process is too high, resulting in high prices of polysaccharide products, such as Column chromatography, chromatographic separation and other links; (4) The production cycle is long and the operation continuity is poor, which affects the output efficiency; (5) The increase in the purity of polysaccharides causes a decrease in yield

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Raw material selection and pretreatment: Take shiitake mushrooms with water content ≤ 10% and coarsely pulverize them, pass through a 60-mesh sieve, then add 15 times of water and soak for 30 minutes;

[0024] (2) Flash extraction: take the shiitake mushroom soaking liquid and put it into a flash extractor for extraction, the extraction conditions are that the extraction speed is 20000r / min, the extraction time is 1min, extraction is performed twice, and the extraction temperature is 20°C;

[0025] (3) centrifugation: the extract is centrifuged at a high speed at a speed of 6000r / min to obtain a supernatant;

[0026] (4) Deproteinization: adding 1% neutral protease to the supernatant to carry out enzymatic deproteinization;

[0027] (5) Microfiltration: the deproteinized solution uses a 0.2 μm microfiltration membrane to remove solid suspended matter such as crude fiber and pectin;

[0028] (6) Reverse osmosis concentration: use polyamide composite reverse osmosis ...

Embodiment 2

[0031] (1) Raw material selection and pretreatment: Take shiitake mushrooms with a water content of ≤10% and coarsely crush them, pass through a 30-mesh sieve, and then add 10 times the amount of water to soak for 10 minutes;

[0032] (2) Flash extraction: take the shiitake mushroom soaking liquid and put it into a flash extractor for extraction, the extraction conditions are that the extraction speed is 1000r / min, the extraction time is 3min, the extraction is 3 times, and the extraction temperature is 55°C;

[0033] (3) centrifugation: the extract is centrifuged at a speed of 3500r / min to obtain a supernatant;

[0034] (4) Deproteinization: adding 1% neutral protease to the supernatant to carry out enzymatic deproteinization;

[0035] (5) Microfiltration: the deproteinized solution uses a 0.5 μm microfiltration membrane to remove solid suspended matter such as crude fiber and pectin;

[0036] (6) Reverse osmosis concentration: use polyamide composite reverse osmosis functio...

Embodiment 3

[0039] (1) Raw material selection and pretreatment: Take mushroom stalks with water content ≤ 10% and coarsely pulverize them, pass through a 40-mesh sieve, then add 15 times of water and soak for 25 minutes;

[0040] (2) Flash extraction: take the mushroom stalk soaking liquid and put it into a flash extractor for extraction. The extraction conditions are that the extraction speed is 10000r / min, the extraction time is 2min, the extraction is performed twice, and the extraction temperature is 35°C;

[0041] (3) centrifugation: the extract is centrifuged at a speed of 5000r / min to obtain a supernatant;

[0042] (4) Deproteinization: adding 1% neutral protease to the supernatant to carry out enzymatic deproteinization;

[0043] (5) Microfiltration: the deproteinized solution uses a 0.3 μm microfiltration membrane to remove solid suspended matter such as crude fiber and pectin;

[0044] (6) Reverse osmosis concentration: use polyamide composite reverse osmosis functional membran...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of lentinus edodes, and provides a method for rapidly extracting polysaccharide from lentinus edodes. The method is characterized in that lentinus edodes or stems thereof are subjected to crushing, soaking, flash extracting, extract liquid centrifuging, deproteinizing, micro-filtrating, reverse osmosis, and microwave vacuum drying, such that lentinus edodes polysaccharide is obtained. The method comprises the specific steps that: raw material selection and pretreatment are carried out, wherein dried lentinus edodes or stems thereof are crushed and soaked; flash extracting is carried out, wherein the lentinus edodes soaking liquid is extracted by using a flash extractor; centrifugation is carried out, wherein the extract liquid is centrifuged, such that a supernatant is obtained; deproteinizing is carried out, wherein neutral protease is added into the supernatant, and enzyme-method deproteinizing is carried out; micro-filtrating is carried out, wherein suspended solids such as crude fiber and pectin are removed from the deproteinized liquid by using a 0.2-0.5mum microfiltration membrane; reverse osmosis concentration is carried out, wherein the micro-filtrated liquid is subjected to reverse osmosis concentration; and microwave vacuum drying is carried out, wherein the polysaccharide concentrated liquid is subjected to microwave vacuum drying, such that the lentinus edodes polysaccharide is obtained. The technical scheme provided by the invention has reasonable process configuration, such that operation time is effectively shortened, and operation efficiency is improved. Process linkage automation degree is high, such that semi-continuous operation can be realized. No organic solvent pollution is caused, and the method is environment-friendly. The method has low integral operation energy consumption, and is economical and feasible.

Description

technical field [0001] The invention relates to the field of shiitake mushrooms, and relates to a method for rapidly extracting polysaccharides from shiitake mushrooms. Background technique [0002] Lentinus edodes belongs to Basidiomycetes, Agaricaceae, Agaricaceae, Lentinus edodes. Its scientific name is Lentinus edodes. It is the second largest edible fungus in the world after Agaricus bisporus. . China's shiitake mushroom export trade volume has gradually increased in the past 10 years, and currently accounts for more than 80% of the world, ranking first in the world. The production of shiitake mushrooms in our country has been in the forefront of the world. Shiitake mushrooms are rich in nutrients, and the lentinan among them has various physiologically active functions such as anti-tumor, anti-oxidation, anti-virus, and blood lipid-lowering. It can be used to prevent and treat various diseases. Therefore, it is widely used in the fields of medicine and food health ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 杨荣杨奇高虹史德芳周明杨德张佳蒋杰杨光黄道忠周保国
Owner RONGSHENGLONG SUIZHOU FOOD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap