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Screening method of pseudomonas solanacearum bacteriophage resource

A screening method and technology for bacterial wilt bacteria, applied in the directions of viruses/phages, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as the inability to effectively control tobacco bacterial wilt disease, and achieve the effect of simple equipment and resources

Inactive Publication Date: 2015-04-29
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

In tobacco leaf production, although comprehensive measures such as planting resistant tobacco varieties, using chemical agents, improving soil structure, and rational crop rotation can reduce the losses caused by tobacco bacterial wilt to a certain extent, they are still not effective. To control the occurrence of bacterial wilt in tobacco, it is therefore expected to develop safer and more effective methods and strategies to control bacterial wilt

Method used

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  • Screening method of pseudomonas solanacearum bacteriophage resource
  • Screening method of pseudomonas solanacearum bacteriophage resource
  • Screening method of pseudomonas solanacearum bacteriophage resource

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Embodiment Construction

[0013] Below in conjunction with example the present invention is described in further detail, but example is not limitation of the present invention.

[0014] 1 material.

[0015] Sterile water, R. solanacearum strains, CPG medium, agar powder, low-melting point agar, petri dish with a diameter of 9 cm, temperature-controlled incubator, plastic wrap, absorbent paper, inoculation bad, pipette, spectrophotometer, scalpel , alcohol lamps, lighters, ultra-clean workbenches, autoclaves, etc. See Table 1 for the source and host information of R. solanacearum strains.

[0016] 2 The implementation steps of the present invention are as follows:

[0017] First, take soil samples and sample processing. Tobacco planting soil was collected from the Fuquan Base of Guizhou Tobacco Research Institute, and 2.0 g of tobacco planting soil (between diseased plants and healthy plants) where tobacco bacterial wilt occurred (see figure 1 ), soaked in 10ml, centrifuged to take the superna...

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Abstract

The invention discloses a screening method of a pseudomonas solanacearum bacteriophage resource. The screening method comprises the following steps: S1, taking a soil sample, treating the sample, and filtering by a filter membrane to obtain a filtrate; S2, culturing a pseudomonas solanacearum strain to obtain a pseudomonas solanacearum liquid; S3, co-culturing the filtrate in the S1 and the pseudomonas solanacearum in the S2; and S4, selecting and storing bacteriophage plaque, wherein the used material comprises sterile water, the pseudomonas solanacearum strain, a CPG culture medium, agar powder and low-melting-point agar. The CPG culture medium comprises a CPG solid culture medium and a CPG liquid culture medium. The method can be applied to screening the pseudomonas solanacearum bacteriophage resource from tobacco-planting soil, bacterial wilt soil and sewage and providing and collecting the pseudomonas solanacearum bacteriophage resource for researching prevention and control of tobacco bacterial wilt by using the pseudomonas solanacearum bacteriophage.

Description

technical field [0001] The invention belongs to the technical field of crop bacterial disease prevention and control, and in particular relates to a screening method for tobacco Ralstonia solanacearum phage resources. Background technique [0002] Tobacco bacterial wilt is caused by the gram-negative bacterium Ralstia solanacearum ( Ralstonia solanacearum , referred to as Ralstonia solanacearum), is one of the main diseases harmful to tobacco. Ralstonia solanacearum is widely distributed, has complex species, numerous hosts, and rich genetic diversity. It can infect more than 200 kinds of plants in more than 50 families, including flue-cured tobacco, potato, tomato, pepper, banana and other important crops. In tobacco leaf production, although comprehensive measures such as planting resistant tobacco varieties, using chemical agents, improving soil structure, and rational crop rotation can reduce the losses caused by tobacco bacterial wilt to a certain extent, they are stil...

Claims

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Application Information

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IPC IPC(8): C12N7/00A01P1/00C12R1/92
Inventor 蔡刘体陆宁石俊雄
Owner GUIZHOU TOBACCO SCI RES INST
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