Bacillus amyloliquefaciens FQS38 and application thereof

A technology of amylolytic bacillus and bacillus, applied in the direction of application, bacteria, and chemicals for biological control, etc., can solve the problems of improper production and medication, inability to take care of two diseases, poor control effect, etc., and achieve good control effect, Human and animal safety, growth promotion effect

Inactive Publication Date: 2013-05-08
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported biocontrol fungicides for bacterial wilt mainly include R. solanacearum and Bacillus, etc., but few biocontrol fungicides related to pituitary necrosis disease have been reported, and the biocontrol fungi

Method used

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  • Bacillus amyloliquefaciens FQS38 and application thereof
  • Bacillus amyloliquefaciens FQS38 and application thereof
  • Bacillus amyloliquefaciens FQS38 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Isolation and identification of Bacillus amyloliquefaciens FQS38

[0019] In September 2011, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain was isolated from tomato rhizosphere soil in Liuhe Base of Jiangsu Academy of Agricultural Sciences by gradient dilution method. The specific method is: collect 10g of rhizosphere soil, add it to a 90mL sterilized water triangular flask, shake and cultivate for 30min (150rpm / min), and make a 1:10 (soil: water) suspension, then remove 1mL of the suspension, and place in a 9mL sterile water triangular flask, and so on, to make 10 -5 , 10 -6 Dilution solution; 330ul was taken from each of them, and coated with YPGA plate, and each treatment was repeated 3 times. Place the above plate at a constant temperature of 28°C for 2 days, pick a single colony and transfer it to a YPGA plate for streaking and purification, and store the purified strain in glycerol at -70°C.

[0020] When the isolated strain was cultured on the ...

Embodiment 2

[0023] Inhibitory effect of Bacillus amyloliquefaciens FQS38 on Ralstonia solanacearum and Necroptosis of tomato.

[0024] Pathogen strains tested:

[0025] Ralstonia solanacearum and Pseudomonas corrugata were isolated and preserved by the Biocontrol Department of Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Ralstonia solanacearum was cultured with NA medium (3.0 g of beef extract, 1 g of yeast extract, 5 g of peptone, 10 g of glucose, 20 g of agar, and 1000 mL of distilled water) at 28°C for 2 days with shaking (150 rpm / min), and set aside. Tomato pith necrosis bacteria were cultured with KB medium (20g of peptone, 10mL of glycerol, 1.5g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate heptahydrate, 1000mL of water, 15g of agar was added to the solid medium) for 2 days and set aside.

[0026]Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FQS38 screened by Jiangsu Academy of Agricultural Sciences was activated on YPGA medium, t...

Embodiment 3

[0030] Production of Antibacterial Related Substances from Bacillus amyloliquefaciens FQS38

[0031] Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Chitinase detection medium: colloidal chitin 15g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O0.01g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, 20g agar, dilute to 1000mL, pH7.0~7.2. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH 2 PO 4 1g, 20g agar, dilute to 1000mL, pH7.0. Siderophilic detection medium: CAS60.5mg, 10mL ferric iron solution (1mmol L-1FeCl 3 · 6H2O), HDTMA72.9g, agar 20g, dilute to 1000mL, pH7.0.

[0032] The preparation of the Bacillus amyloliquefaciens FQS38 culture solution was the same as in Example 2.

[0033] Protease detection: place a 5mm sterilized filter paper sheet in the center of the protease detection medium plate, and drop 5 μL of antagonistic bacteria solution on each filter paper sheet. Repeat 4 di...

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Abstract

The invention relates to an antibiological inoculant, wherein an effective active ingredient of the antibiological inoculant is composed of bacillus amyloliquefaciens FQS38. The antibiological inoculant is characterized by being capable of conducting colonization on the roots of tomatoes, promoting the growth of tomatoes as well as preventing and treating the soil-borne disease of the tomatoes. In addition, the antibiological inoculant can secrete protease, cellulase, siderophores, auximones, gibberellins and other antibacterial and growth promoting ingredients. The antibiological inoculant is an aqueous solution which mainly prepared from the following ingredients in proportion: bacillus amyloliquefaciens FQS38 fermentation liquor (the bacterium content is 1*1010-2*1010cfu/mL) and 0.1 to 0.5% of corrosion remover with the pH value being 5.0 to 6.0. After being diluted, the antibiological inoculant is filled into the roots of the tomatoes in the colonization process so as to prevent and treat the bacterial wilt and the marrow necrosis disease of the tomatoes; and then the subsequent root filling is carried out according to the water-fertilizer rule. After the finished drug is diluted, the effective bacterium content is 106 to 108cfu/mL. The antibiological inoculant has the advantages that the bacillus amyloliquefaciens FQS38 has a good prevention and treatment effect on the bacterial soil-borne disease of the tomatoes, can promote the growing of the tomatoes, has no toxicity and pathogenicity, is safe for people and livestock and is environment-friendly, thereby being possibly applied to field production.

Description

technical field [0001] The present invention relates to a strain of biocontrol bacteria and its application, namely Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FQS38 and its growth promoting effect on tomato and the treatment of two important bacterial soil-borne diseases of tomato (bacterial wilt and pith necrosis). prevention and control. Background technique [0002] Facility tomato planting plays an increasingly important role in my country's agricultural and rural economic development, and has become a pillar industry in modern agricultural production and an important way for farmers to increase their income. In recent years, frequent outbreaks of soil-borne diseases (fusarium wilt, bacterial wilt, bacterial pith necrosis, root rot, etc.) caused by continuous cropping of a single species have seriously threatened the sustainable development of vegetable production in facilities. The main reason for the occurrence of soil-borne diseases is that the continuou...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/07
Inventor 乔俊卿刘邮洲梁雪杰聂亚锋陈志谊刘永锋
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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