Bacillus amyloliquefaciens FQS38 and application thereof
A technology of amylolytic bacillus and bacillus, applied in the direction of application, bacteria, and chemicals for biological control, etc., can solve the problems of improper production and medication, inability to take care of two diseases, poor control effect, etc., and achieve good control effect, Human and animal safety, growth promotion effect
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Embodiment 1
[0018] Isolation and identification of Bacillus amyloliquefaciens FQS38
[0019] In September 2011, Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) strain was isolated from tomato rhizosphere soil in Liuhe Base of Jiangsu Academy of Agricultural Sciences by gradient dilution method. The specific method is: collect 10g of rhizosphere soil, add it to a 90mL sterilized water triangular flask, shake and cultivate for 30min (150rpm / min), and make a 1:10 (soil: water) suspension, then remove 1mL of the suspension, and place in a 9mL sterile water triangular flask, and so on, to make 10 -5 , 10 -6 Dilution solution; 330ul was taken from each of them, and coated with YPGA plate, and each treatment was repeated 3 times. Place the above plate at a constant temperature of 28°C for 2 days, pick a single colony and transfer it to a YPGA plate for streaking and purification, and store the purified strain in glycerol at -70°C.
[0020] When the isolated strain was cultured on the ...
Embodiment 2
[0023] Inhibitory effect of Bacillus amyloliquefaciens FQS38 on Ralstonia solanacearum and Necroptosis of tomato.
[0024] Pathogen strains tested:
[0025] Ralstonia solanacearum and Pseudomonas corrugata were isolated and preserved by the Biocontrol Department of Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Ralstonia solanacearum was cultured with NA medium (3.0 g of beef extract, 1 g of yeast extract, 5 g of peptone, 10 g of glucose, 20 g of agar, and 1000 mL of distilled water) at 28°C for 2 days with shaking (150 rpm / min), and set aside. Tomato pith necrosis bacteria were cultured with KB medium (20g of peptone, 10mL of glycerol, 1.5g of dipotassium hydrogen phosphate, 1.5g of magnesium sulfate heptahydrate, 1000mL of water, 15g of agar was added to the solid medium) for 2 days and set aside.
[0026]Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) FQS38 screened by Jiangsu Academy of Agricultural Sciences was activated on YPGA medium, t...
Embodiment 3
[0030] Production of Antibacterial Related Substances from Bacillus amyloliquefaciens FQS38
[0031] Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Chitinase detection medium: colloidal chitin 15g, MgSO 4 ·7H 2 O0.5g, FeSO 4 ·7H 2 O0.01g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, 20g agar, dilute to 1000mL, pH7.0~7.2. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH 2 PO 4 1g, 20g agar, dilute to 1000mL, pH7.0. Siderophilic detection medium: CAS60.5mg, 10mL ferric iron solution (1mmol L-1FeCl 3 · 6H2O), HDTMA72.9g, agar 20g, dilute to 1000mL, pH7.0.
[0032] The preparation of the Bacillus amyloliquefaciens FQS38 culture solution was the same as in Example 2.
[0033] Protease detection: place a 5mm sterilized filter paper sheet in the center of the protease detection medium plate, and drop 5 μL of antagonistic bacteria solution on each filter paper sheet. Repeat 4 di...
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