Method for identifying resistance to tobacco bacterial wilt during seedling stage

A tobacco bacterial wilt and identification method technology, applied in the field of crop disease resistance identification, can solve the problem of unstable inoculation effect of capillary thorn stem penetration method and leaf cutting inoculation method, large amount of R. solanacearum suspension required, and tobacco material Small quantity and other problems, to save site and test costs, shorten the identification cycle, reduce the effect of hydroponic area

Inactive Publication Date: 2009-08-05
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

However, this method has the following disadvantages: the identification period is long, and it can only be identified once a year; the area of ​​the required disease nursery is large, and the number of tobacco materials used for identification is small; the number of bacterial suspensions of R. solanacearum is large; the incidence is uneven
Among them, root injury perfusion inoculation c

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  • Method for identifying resistance to tobacco bacterial wilt during seedling stage
  • Method for identifying resistance to tobacco bacterial wilt during seedling stage

Examples

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Embodiment 1

[0017] The invention discloses a method for identifying tobacco bacterial wilt resistance at the seedling stage. A thermal insulation and heating floating pool is built in a glass greenhouse or a plastic greenhouse. The bottom of the pool is covered with a foamed plastic board about 4 cm thick, covered with 2 layers of plastic film, and the entire pool wall is surrounded by foamed plastic boards for insulation. One end of the seedling pond is equipped with a temperature-controlled electric heating rod (product of Zhongshan Chaoxin Electric Appliance Factory) and a pool water circulation pump (product of Guangdong Haili Group Co., Ltd.). The temperature control electric heating rod and the pool water circulation pump are linked through the temperature control device. The pool water is deep well water or tap water. The water depth is 10cm. The water temperature was set at 26.5°C according to the optimum onset temperature of bacterial wilt and the soil temperature of Yunnan end...

Embodiment 2

[0019] Repeat Example 1 with the following differences: the depth of the pool water is 5 cm, and the temperature is set at 25° C. After cutting the leaves twice, the tobacco seedlings have a plant height of 9 cm and a seedling age of 45 days. 25 plants are selected for each material to be identified. The nitrogen concentration of the nutrient solution in the floating pool is controlled at 100mg / kg. The bacterial lawn was scraped off, inoculated into 1 / 10 BG culture solution, and cultured with shaking at 28°C for 36 hours.

Embodiment 3

[0021] Repeat Example 1, with the following differences: the water depth of the pool is 20cm, and the temperature is set at 28°C. After cutting the leaves twice, the tobacco seedlings are 11cm in height and 55 days in age, and 200 plants are selected for each material to be identified. The nitrogen concentration of the nutrient solution in the floating pool is controlled at 250mg / kg.

[0022] The following is the correlation analysis of the results of constant temperature hydroponic identification of bacterial wilt at the seedling stage, identification of potted plants at the seedling stage, and identification of natural disease in the field

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Abstract

The invention relates to a seedling stage identification method for the resistance of tobacco to the bacterial wilt. The seedling stage identification method comprises the steps of heat preservation pool construction, pool water heating and circulation, wilt bacterium inoculation, tobacco seedling, disease state investigation and grading. The method replaces a conventional field identification result with a seedling stage identification result, identifies a great amount of material in a smaller area, carries out the identification a plurality of times annually, improves the reliability of the result, shortens the identification cycle, saves the site and test cost, and covers little floor space. The seedling stage identification method for the resistance of tobacco to the bacterial wilt is characterized by rapid disease development, good result repeatability, and the like. The seedling stage identification method for the resistance of tobacco to the bacterial wilt can effectively remove the interference on the resistance result caused by soil temperature difference, strain pathogenicity difference, strain quantity difference and other diseases, thereby benefiting the recurrence and comparison of results from different years and different labs. The difference between the attack rates of infection resistant varieties and the disease indexes is obvious; and the appraisal result of the resistance of the variety is basically consistent with the perennial resistance appraisal result obtained by others. The method has greater application potential in the resistance identification and resistance heredity rule study of disease resistant resources and breeding medium materials.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, and in particular relates to a method for identifying tobacco bacterial wilt resistance at seedling stage. Background technique: [0002] Tobacco bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum (Ralstonia solanacearum for short), which mainly invades from roots. Breeding and planting disease-resistant varieties is an economical and effective control measure to control the occurrence and prevalence of bacterial wilt. However, in research related to disease resistance breeding, it is necessary to accurately and quickly evaluate the resistance performance of a large number of materials. The existing identification methods for tobacco bacterial wilt resistance mainly include the following two methods: 1) the method of artificial inoculation in field disease gardens. This method can better reflect the resistance of the variety. However, this me...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/06A01G31/06C12N1/20
CPCY02P60/21
Inventor 刘勇陈学军李文正许文李梅云徐照丽卢秀萍李永平
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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