Method for identifying resistance to tobacco bacterial wilt during seedling stage

Abstract

The invention relates to a seedling stage identification method for the resistance of tobacco to the bacterial wilt. The seedling stage identification method comprises the steps of heat preservation pool construction, pool water heating and circulation, wilt bacterium inoculation, tobacco seedling, disease state investigation and grading. The method replaces a conventional field identification result with a seedling stage identification result, identifies a great amount of material in a smaller area, carries out the identification a plurality of times annually, improves the reliability of the result, shortens the identification cycle, saves the site and test cost, and covers little floor space. The seedling stage identification method for the resistance of tobacco to the bacterial wilt is characterized by rapid disease development, good result repeatability, and the like. The seedling stage identification method for the resistance of tobacco to the bacterial wilt can effectively remove the interference on the resistance result caused by soil temperature difference, strain pathogenicity difference, strain quantity difference and other diseases, thereby benefiting the recurrence and comparison of results from different years and different labs. The difference between the attack rates of infection resistant varieties and the disease indexes is obvious; and the appraisal result of the resistance of the variety is basically consistent with the perennial resistance appraisal result obtained by others. The method has greater application potential in the resistance identification and resistance heredity rule study of disease resistant resources and breeding medium materials.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, and in particular relates to a method for identifying tobacco bacterial wilt resistance at seedling stage. Background technique: [0002] Tobacco bacterial wilt is a soil-borne disease caused by Ralstonia solanacearum (Ralstonia solanacearum for short), which mainly invades from roots. Breeding and planting disease-resistant varieties is an economical and effective control measure to control the occurrence and prevalence of bacterial wilt. However, in research related to disease resistance breeding, it is necessary to accurately and quickly evaluate the resistance performance of a large number of materials. The existing identification methods for tobacco bacterial wilt resistance mainly include the following two methods: 1) the method of artificial inoculation in field disease gardens. This method can better reflect the resistance of the variety. However, this me...

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Problems solved by technology

However, this method has the following disadvantages: the identification period is long, and it can only be identified once a year; the area of ​​the required disease nursery is large, and the number of tobacco materials used for identification is small; the number of bacterial suspensions of R. solanacearum is large; the incidence is uneven

Among them, root injury perfusion inoculation can cause severe infection, the inoculation effect is relatively stable, and the correlation with the natural disease identification results of the diseased area is high. Large, the inoculation effect of capillary prickly stem infiltration method and leaf cutting inoculation method is unstable, and the correlation with the resistance performance of the variety in the diseased area is low

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