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Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof

A tomato bacterial wilt and organic fertilizer technology, applied in the field of intensive agricultural production, can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, waste of raw materials, etc., to improve the ability of colonization and function, growth and the effect of enhancing resilience and reducing morbidity

Active Publication Date: 2010-03-03
南京农业大学资产经营有限公司
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AI Technical Summary

Problems solved by technology

[0003] On the other hand, the incineration of rice, wheat, corn, rapeseed and other crop straws, and the disposal of livestock and poultry manure after large-scale breeding not only seriously pollute the environment, but also greatly waste the organic fertilizer and bio-organic fertilizer products. Raw materials; a large number of nutrient resources (C, N, P, K, S and trace elements) are lost outside the soil-plant system, which obviously weakens the ability of my country's agricultural sustainable development

Method used

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  • Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof
  • Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof
  • Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof

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Embodiment Construction

[0023] 1. Isolation and identification of strains

[0024] Collect the plants and surrounding soil that have experienced continuous cropping obstacles and tomato bacterial wilt symptoms, and use SMSA bacterial wilt selective medium to obtain pathogenic strains and verify their pathogenicity. Collect the rhizosphere soil of healthy tomato plants in continuous cropping tomato bacterial wilt disease, and use the NA-improved medium, PDA-improved medium, and LB-improved medium to carry out antagonism tests with pathogenic bacteria as indicator bacteria by confrontation method and Oxford cup method respectively. And preliminary screening of antagonistic strains. The high-efficiency antagonistic strain NJQL-18 was finally obtained and identified through pot experiments, and the strain was freeze-dried and stored at -20°C.

[0025] Strain NJQL-18 belongs to Bacillus amyloliquefaciens, and its main biological characteristics are Gram-positive G + , the cell shape is short rod-shaped,...

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Abstract

The invention relates to antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and a microbial organic fertilizer produced thereby, which belong to the technologies of agricultural intensified production. The antagonistic bacteria NJQL-18 with the significant antagonistic effect for pathogenic bacteria of the tomato bacterial wilt is obtained by separation, solidfermentation is further carried out on the antagonistic bacteria and organic materials for preparing the microbial organic fertilizer, the fertilizer contains more than 1 multiplied by 10<8> / g of NJQL-18, the content of total nitrogen is 4-5% (more than 90% is organic nitrogen), the total content of nutrients of nitrogen, phosphorus and potassium is 6-10%, and the content of organic matters is 30-35%. Tests show that the microbial organic fertilizer can lead the antagonistic bacteria to be rapidly propagated after being applied into the soil and form heterotic groups in the soil, and the biological prevention efficiency in the soil with the serious tomato bacterial wilt can be more than 80%. The long-term application of the microbial organic fertilizer in the soil without the formation ofcontinuous cropping obstacles can prevent the occurrence of the continuous cropping tomato bacterial wilt to a great extent.

Description

1. Technical field [0001] The invention relates to an antagonistic bacteria capable of preventing continuous-cropping tomato bacterial wilt and its microbial organic fertilizer, which belongs to the agricultural intensive production technology and is specially used for overcoming and eliminating the problem of continuous-cropping tomato bacterial wilt. 2. Background technology [0002] Tomato bacterial wilt is a bacterial soil-borne disease of vascular bundles caused by Ralstonia solanacearum. In 1906, American Simth scholars discovered and reported the disease for the first time. So far, bacterial wilt exists in all countries and regions except Antarctica. Laueria is divided into five physiological races and six biochemical races. All the races have certain pathogenicity to tomato, and race 1 has the strongest pathogenicity to tomato. In my country, tomato bacterial wilt mainly occurs in the warm and humid south. With the warming of the climate and the development of high...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P1/00C05G3/00C05F11/08C05F17/00C12R1/07
CPCY02W30/40
Inventor 沈其荣韦中徐阳春杨兴明沈标
Owner 南京农业大学资产经营有限公司
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