Antagonistic bacteria preventing and removing continuous cropping tomato bacterial wilt and microbial organic fertilizer thereof
A tomato bacterial wilt and organic fertilizer technology, applied in the field of intensive agricultural production, can solve the problems of weakening the ability of sustainable agricultural development, loss of nutrient resources, waste of raw materials, etc., to improve the ability of colonization and function, growth and the effect of enhancing resilience and reducing morbidity
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[0023] 1. Isolation and identification of strains
[0024] Collect the plants and surrounding soil that have experienced continuous cropping obstacles and tomato bacterial wilt symptoms, and use SMSA bacterial wilt selective medium to obtain pathogenic strains and verify their pathogenicity. Collect the rhizosphere soil of healthy tomato plants in continuous cropping tomato bacterial wilt disease, and use the NA-improved medium, PDA-improved medium, and LB-improved medium to carry out antagonism tests with pathogenic bacteria as indicator bacteria by confrontation method and Oxford cup method respectively. And preliminary screening of antagonistic strains. The high-efficiency antagonistic strain NJQL-18 was finally obtained and identified through pot experiments, and the strain was freeze-dried and stored at -20°C.
[0025] Strain NJQL-18 belongs to Bacillus amyloliquefaciens, and its main biological characteristics are Gram-positive G + , the cell shape is short rod-shaped,...
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