Bacillus subtilis PTS-394 for preventing and controlling soil-borne diseases of solanaceous fruit vegetables and application thereof

A technology of PTS-394 and Bacillus subtilis, applied in the direction of application, fungicides, chemicals for biological control, etc., can solve problems such as difficult diagnosis and poor control, reduce drug costs, improve ecological environment, reduce The effect of residue

Active Publication Date: 2012-07-11
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Another object of the present invention is: for the current production of solanaceous vegetables two kinds of important soil-borne diseases (fusarium wilt and bacterial wilt) symptoms are similar, difficult to diagnose and the problem of poor control, screening obtains a plant that is effective against fusarium wilt and bacterial wilt. Bacillus subtilis PTS-394 and its application

Method used

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  • Bacillus subtilis PTS-394 for preventing and controlling soil-borne diseases of solanaceous fruit vegetables and application thereof
  • Bacillus subtilis PTS-394 for preventing and controlling soil-borne diseases of solanaceous fruit vegetables and application thereof
  • Bacillus subtilis PTS-394 for preventing and controlling soil-borne diseases of solanaceous fruit vegetables and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Inhibition of Bacillus subtilis PTS-394 against Fusarium wilt and Ralstonia solanacearum.

[0036] Pathogen strains tested:

[0037] Fusarium wilt of tomato was provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Use PSA medium (200g potato, 20g sucrose, 20g agar, and 1000mL distilled water, pH 5.6-6.6) to culture at 28°C for 5 days, and set aside.

[0038]Tomato solanacearum was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Use NA medium (3.0 g of beef extract, 1 g of yeast extract, 5 g of peptone, 10 g of glucose, 20 g of agar, and 1000 mL of distilled water) at 28°C for 2 days with shaking (150 rpm / min), and set aside.

[0039] The Bacillus subtilis PTS-394 screened by Jiangsu Academy of Agricultural Sciences was activated on NA medium, then transferred into 50 mL NA medium, and cultured at 28° C. for 2 days with shaking (150 rpm / min) for later use. Tomato wilt fungus cake (7mm in diameter) ...

Embodiment 2

[0046] Metabolic secretions of Bacillus subtilis PTS-394-production of antimicrobial-related substances

[0047] Test materials: protease detection medium: skimmed milk powder 100g, agar 20g, dilute to 1000mL. Chitinase detection medium: colloidal chitin 15g, MgSO4 7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.01g, K 2 HPO 4 0.7g, KH 2 PO 4 0.3g, 20g agar, dilute to 1000mL, pH7.0~7.2. Cellulase detection medium: peptone 10g, yeast powder 10g, sodium carboxymethylcellulose 10g, NaCl 5g, KH 2 PO 4 1g, 20g agar, dilute to 1000mL, pH7.0. Siderophilic detection medium: CAS 60.5g, 10mL ferric iron solution (1mmol.L -1 FeCl 3 ·6H 2 O), HDTMA 72.9g, agar 20g, be settled to 1000mL, pH7.0.

[0048] The Bacillus subtilis PTS-394 screened by Jiangsu Academy of Agricultural Sciences was activated on NA medium, then transferred into 50 mL NA culture medium, cultured at 28° C. with shaking (150 rpm / min) for 2 days, and set aside.

[0049] Protease detection: place a 5mm sterilized filter pa...

Embodiment 3

[0056] Preparation of 20 billion bacillus subtilis PTS-394 water agent

[0057] A. Strain preparation and activation.

[0058] The NA medium (same as in Example 1) was sterilized by the conventional method, made into a slant, and after pre-cultured for 2 days, was inoculated with Bacillus subtilis PTS-394, culture temperature: 28° C.; culture time: 2 days.

[0059] B, liquid fermentation.

[0060] Primary fermentation: the above-mentioned cultivated test tube slant, with an inoculation ring to transfer a ring of bacterial lawn into 500mLNA culture solution (same as in Example 1), and transfer 2 bottles altogether. Shake (150rpm / min) culture at 28°C for 2 days and set aside.

[0061] Secondary fermentation: medium formula: bean cake powder 1.0%, soybean powder 0.5%, yeast powder 0.15%, corn flour 2.0%, fish meal 1.0%, NaCl 0.05%, CaCO 3 0.3%. The pH before elimination is 7.5. Feed 1 ton into a 2 ton fermenter. Sterilize at 121°C for 30 minutes. When cooling to about 30°C...

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Abstract

The invention relates to a biocontrol bacterial agent. An effective active constituent consists of bacillus subtilis PTS-394. The biocontrol bacterial agent is characterized in that: the biocontrol bacterial agent is aqueous solution or wettable powder. The aqueous solution comprises the following components in percentage by weight: bacillus subtilis PTS-394 with 2 *1,010 cfu/mL of content of bacteria, 0.5 percent of NNO (sodium methylene naphthalene sulfonate), 6 percent of SDBS (sodium dodecyl benzene sulfonate) and 0.1 percent of sodium benzoate. The wettable powder comprises the following components in percentage by weight: 40 percent of bacillus subtilis PTS-394 viable bacterium raw powder with 5 *1,010 cfu/g of content of bacteria, 7 percent of NNO (sodium methylene naphthalene sulfonate), 3 percent of polyethylene glycol, 5 percent of SDBS (sodium dodecyl benzene sulfonate) and 45 percent of light calcium carbonate. The biocontrol bacterial agent is poured into vegetable roots when applied and is used for preventing and controlling vegetable soil-borne diseases such as solanaceous fruit vegetable blight and bacterial wilt mainly; and the content of effective agent components is between 104 and 108 cfu/mL or between 104 and 108 cfu/g after finished product agents are diluted. The biocontrol bacterial agent has the advantages that: the aims of preventing and controlling two kinds of soil-borne diseases by means of one kind of biocontrol bacterial agent are fulfilled by introducing the bacillus subtilis PTS-394 which is safe to people and livestock and has better inhibition effect on the solanaceous fruit vegetable blight and the bacterial wilt; the using amount of chemical agents is reduced, and the agent cost is reduced; and the ecological environment is improved, and the residues of the chemical agents in the vegetables are reduced and the same time.

Description

technical field [0001] The invention relates to a biocontrol agent and its application, in particular to a bacillus subtilis PTS-394 and its application in preventing and treating two important soil-borne diseases (fusarium wilt and bacterial wilt) of solanaceous vegetables. Background technique [0002] With the adjustment of the planting industry structure, the planting area of ​​protected vegetables has increased year by year. The high-yield, continuous cropping, and diversified protected vegetable planting layout provides sufficient nutrition and breeding conditions for pests and diseases. Therefore, major diseases of facility vegetables have frequently broken out in recent years. At present, the prevention and control of diseases in the production of protected vegetables mainly relies on chemical pesticides. This not only causes serious pollution to the ecological environment, but also directly increases the residues of toxic chemicals in vegetables, causing serious ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 刘邮洲陈志谊乔俊卿聂亚峰梁雪杰
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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