Bacillus subtilis capable of decomposing phosphorus and high producing polyglutamic acid and application thereof

A Bacillus subtilis, polyglutamic acid technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of undisclosed phosphorus solution, no phosphorus solution, etc., and achieve a broad antibacterial spectrum and broad-spectrum antibacterial effect. Effect

Active Publication Date: 2018-06-01
FOSHAN YANHUI BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to whether glutamic acid is added to the medium, γ-PGA-producing bacteria are divided into two types: non-glutamate-dependent bacteria and glutamate-dependent bacteria; Chen Liang disclosed a co-production of multiple antibacterial lipopeptides in patent number 2015101652435 and γ-polyglutamic acid Bacillus subtilis strain, which is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC NO.9377, but this bacterial strain does not have the effect of dissolving phosphorus. There is no published Bacillus subtilis that not only has phosphorus solubilization, but also has the ability to prevent and control various plant diseases and high-yield γ-polyglutamic acid

Method used

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  • Bacillus subtilis capable of decomposing phosphorus and high producing polyglutamic acid and application thereof
  • Bacillus subtilis capable of decomposing phosphorus and high producing polyglutamic acid and application thereof
  • Bacillus subtilis capable of decomposing phosphorus and high producing polyglutamic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Isolation of Bacillus with Phosphorus Solubilizing Effect

[0018] 167 strains of Bacillus were isolated from Hunan, Changping, Hebei, Liaoning and other places, 101 of which had phosphorus-solubilizing activity.

[0019] 18 strains with high phosphorus-solubilizing intensity were screened. By measuring the aqueous solution phosphorus in the shake flask fermentation liquid of 18 bacillus strains, the decomposition ability of each bacterial strain to inorganic phosphorus and organic phosphorus was determined, as shown in Table 1. It can be seen from the results that the decomposition of inorganic phosphorus and the decomposition of organic phosphorus are not positively correlated. Based on the comprehensive understanding of organic phosphorus and inorganic phosphorus, we determined that five of them, JCD-H-12, JCD-H-16, JCD-Q-3, JCD-L-4, and JCD-L-16, had strong phosphorus-dissolving ability. The strain of action. Use the following two kinds of organic phosp...

Embodiment 2

[0026] Implementation 2 Screening of the antibacterial test of Bacillus with strong phosphorus-solubilizing effect

[0027] Put JCD-H-12, JCD-H-16, JCD-Q-3, JCD-L-4, JCD-L-16 in Pythium spp.,

[0028] Rice blast (Pyriculavia oryzae cav.), Pepper early blight (Alternaria solanipepper), Tomato bacterial wilt (Psevdomonas solanacearum), Rhizoctonia solani (Rhizoctonia solani), Powdery mildew (Erisyphe spp.),

[0029] Tobacco black shank (Phytophthora parasitica var.nicotianae), strawberry gray mold (Botrytiscinera Pers.ex Fr.) and other pathogenic microorganisms were marked with "crosses" on the plate, and after 72 hours of constant temperature cultivation at 28°C, observe whether there is a zone of inhibition. The results are shown in Table 2 below.

[0030] Table 2 Antibacterial test results of Bacillus with strong phosphorus-solubilizing effect

[0031]

Embodiment 3

[0032] Example 3 Identification of Bacillus subtilis JCD-H-16 and Test of Broad Spectrum Antibiotic Properties

[0033] Through physiological and biochemical tests, Gram-positive bacteria are rod-shaped, endophytic spores, and without flagella; they form milky white colonies on beef peptone medium, with smooth surfaces and irregular edges. Glucose fermentation test positive, starch hydrolysis test positive, gelatin liquefaction test positive, V.P reaction positive, contact enzyme reaction positive, methyl red reaction positive, nitrate reduction test positive, citrate utilization positive, aerobic growth, optimum pH The temperature is 6-8.5, the optimum temperature for growth is 28-38°C, and it is identified as Bacillus subtilis by 16s rDNA.

[0034] Take a ring of activated bacteria liquid from JCD-H-16 and cross-hatch it on a PDA plate, inoculate pathogenic bacteria at a distance of 3cm from the inoculation line, incubate at 28°C for 7 days, and measure the width of the inhi...

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Abstract

The invention relates to bacillus subtilis with a stronger phosphorus decomposing effect and stronger gamma-polyglutamic acid producing capacity. Furthermore, the bacillus subtilis has a better preventing and treating effect on varieties of plant diseases and a plant growth promoting effect. A preservation serial number of the bacillus subtilis JCD-H-16 with phosphorus decomposing, disease preventing and growth promoting effects disclosed by the invention is CGMCC NO.13663, a preservation date is February 16th in 2017, and the bacillus subtilis has multiple effects of decomposing phosphorus, preventing diseases, promoting growth, high producing gamma-polyglutamic acid, increasing yield, improving quality, having a wide antibacterial spectrum and the like. A bacterial strain and a fungicideof the bacillus subtilis disclosed by the invention can effectively prevent and treat powdery mildew, bacterial wilt, gray mold, rice sheath blight disease, pimento anthracnose, damping off and the like; broad-spectrum antibacterial is achieved, and effects are stable and durable; the bacillus subtilis can be applied to developing a bacterial fertilizer and a microbial pesticide preparation integrated with phosphorus decomposing, gamma-polyglutamic acid producing and disease preventing and treating. When the bacterial fertilizer of the bacillus subtilis disclosed by the invention is applied,about one half of a nitrogen fertilizer and a phosphorus fertilizer can be reduced, and an obvious yield increasing effect is further obtained.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers and microbial pesticides, and in particular relates to a bacillus subtilis JCD-H-16 capable of dissolving phosphorus, preventing disease, promoting growth and high-yielding gamma-polyglutamic acid. Background technique [0002] The average content of phosphorus in the earth's crust is about 0.122%. During the process of soil formation, phosphorus migrates to a certain extent. With the progress of soil formation, the content of phosphorus in the soil also decreases. Most of the soil phosphorus content in my country is between 0.043%-0.046%, so in the soil, the phosphorus content is relatively deficient. Since phosphorus is one of the 16 nutrient elements necessary for plant growth and development, crops mainly absorb phosphorus in chemical forms such as phosphate, while phosphorus in soil mainly exists in the state of fixed minerals, accounting for about 70% of the total phosphorus co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C05F11/08C12R1/125
CPCA01N63/10C05F11/08C12N1/205C12R2001/125Y02A40/10
Inventor 林祖端
Owner FOSHAN YANHUI BIOTECH CO LTD
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