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PHBV (polyhydroxylbutyrate valerate) polymer, preparation method and hemostatic material manufactured by using PHBV polymer

A polymer and copolymer technology, applied in the biological field, can solve the problems of increasing the chance of wound infection, endangering life, and decreasing hardness.

Active Publication Date: 2015-03-25
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The incorporation of 3-hydroxyvaleric acid monomer significantly changed the crystal structure of PHBV, resulting in a decrease in hardness, strength, and melting point, but the decomposition temperature did not decrease, and with the addition of 3HV, the crystal regularity of the crystal decreased and Presents different crystalline forms, which have an improvement effect on the performance of PHBV
In surgical trauma and emergency surgery, wound bleeding is a common problem. If the bleeding cannot be effectively stopped in time, it will lead to blood loss, which will damage the health of the body and even endanger life in severe cases.
At the same time, blood is rich in nutrients, and bleeding will increase the chance of wound infection, which will bring health risks
Traditional hemostatic materials such as hemostatic gauze and bandages have certain limitations, such as long hemostasis time and easy adhesion to the wound.

Method used

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  • PHBV (polyhydroxylbutyrate valerate) polymer, preparation method and hemostatic material manufactured by using PHBV polymer
  • PHBV (polyhydroxylbutyrate valerate) polymer, preparation method and hemostatic material manufactured by using PHBV polymer
  • PHBV (polyhydroxylbutyrate valerate) polymer, preparation method and hemostatic material manufactured by using PHBV polymer

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Embodiment 1

[0029]This embodiment provides a PHBV polymer, the polymer is a copolymer of 3-hydroxybutyrate 3HB and 3-hydroxyvaleric acid 3HV, and the main chain of the polymer includes at least fifty poly 3-hydroxy A block of butyric acid and a block of at least fifty poly-3-hydroxyvaleric acid.

[0030] The main chain of the above-mentioned polymer also includes random copolymerization segments of 3-hydroxybutyric acid and 3-hydroxyvaleric acid;

[0031] The molar ratio of comonomers 3HB and 3HV in the polymer is 20-50:80-50, preferably 33-50:67-50;

[0032] The weight average molecular weight of the polymer is 1~3×10 6 , the molecular weight polydispersity coefficient is 1.3-2.2, preferably 1.5-1.8.

[0033] The thermodynamic parameters of the above-mentioned PHBV polymer are as follows: glass transition temperature-15~5℃, preferably-10~3℃; cold crystallization temperature 55~72℃, preferably-62~70℃; melting temperature 132-145℃, Preferably 135-145°C; decomposition temperature 260-275...

Embodiment 2

[0054] This embodiment provides the microstructure analysis of embodiment 1 PHBV

[0055] The purified PHBV sample was dissolved in deuterated chloroform at a concentration of 20 mg / mL, and methylsilane was used as an internal standard. The samples were analyzed by nuclear magnetic resonance (Bruker AVANCE III 500, Germany) at room temperature. 13 Data acquisition for C NMR. The sample recorded a spectral width of 10,000 Hz on the spectrometer, and 32k data points were accumulated 30,000 times 13 C NMR.

[0056] Figure 1A with 1B It is the PHBV microstructure analysis figure provided by the present embodiment, in the figure is 13 3HV NMR spin-splitting peak in the C NMR spectrum. Among them, the 3HV molar content in the random copolymerized PHBV sample is 30.1%, and the 3HV molar content in the highly regular copolymerized PHBV sample is 33.3%, that is, the sample with a special microstructure obtained in this embodiment; the 3HV monomer content of the two is basically t...

Embodiment 3

[0072] This embodiment analyzes the molecular weight and its distribution of the PHBV material, and its thermodynamic properties

[0073] The molecular weight of the above-mentioned highly regular polymer PHBV was determined by gel retardation chromatography (Agilent HPLC 1260, USA). Chloroform was used as the mobile phase at a flow rate of 1.0ml / min. Polystyrene (1.01×10 4 ,2.88×10 4 ,7.48×10 4 ,2.15×10 5 ,5.08×10 5 ,9.91×10 5 , and 3.04×10 6 ) (Agilent, USA) was used as the standard molecular weight to make a standard curve. The results show that the weight-average molecular weight of the highly regular polymer PHBV is 2.0×10 6 , Molecular weight polydispersity coefficient 1.63.

[0074] The thermodynamic analysis of the above highly regular polymer PHBV was carried out using a differential calorimetry scanner (DSC Q2000, USA) and a thermogravimetric analyzer (TGA Q500, USA). The measurement results are as follows: the glass transition temperature is -8.91°C and -0...

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Abstract

The invention discloses a PHBV (polyhydroxylbutyrate valerate) polymer, a preparation method and a hemostatic material manufactured by using the PHBV polymer. The PHBV polymer is a copolymer of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV), and the main chain of the polymer includes at least 50 blocks of poly 3-hydroxybutyrate and at least 50 blocks of poly 3-hydroxyvalerate. The preparation method comprises: inoculating Haloferax mediterranei ES1 strains to a fermentation medium, and automatically feeding 2M hydrochloric acid solution or 2M sodium hydroxide solution under the condition that the fermentation temperature is 37 DEG C, the stirring revolution speed is 300-500r / min and the ventilatory capacity is 1.25: 1vvm to stabilize the pH value at 6.9-7.1; fermenting for 36-48h under the condition that the ventilatory capacity is 1.25: 1vvm and the inner pressure of a tank body is 1 atmosphere; and extracting the PHBV polymer from the fermentation solution.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a PHBV polymer, a preparation method and a hemostatic material made of the PHBV polymer. Background technique [0002] Polyhydroxyalkanoates (Polyhydroxyalkanoates, PHA) are a class of biopolyesters composed of hydroxy fatty acids as monomers, which are produced by a variety of bacteria and some halophilic archaea under unbalanced nutritional conditions. PHA exists as hydrophobic particles in the cell, and it is mainly used as a storage substance for intracellular carbon source, energy and reducing power. PHA has material properties similar to traditional petrochemical plastics, but it can be synthesized from renewable carbon sources and can be completely degraded into the ecological cycle of nature, so it is considered a "green plastic". As a biodegradable plastic, PHA has properties ranging from rigid materials to elastomers, and can be applied to various packaging mat...

Claims

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Application Information

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IPC IPC(8): C08G63/06C12P7/62A61L15/26A61L15/44A61L15/42C12R1/01
Inventor 韩静向华侯靖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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