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Method for synthesizing poly-3-hydroxybutyrate/3-polyhydroxybutyrate from wooden carbon source domesticated sewage mixed with bacteria

A technology of hydroxybutyrate and hydroxyvalerate, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of low recovery rate, waste of biomass resources, etc., and improve production , the effect of increasing carbon source concentration and reducing production cost

Inactive Publication Date: 2018-12-28
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the recovery rate of woody biomass waste in my country is very low, and most of them are disposed of through open burning or landfill, which is a great waste of biomass resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The acclimated sewage was added into the beaker, the initial mixed liquid suspended particle concentration (MLSS) of the sewage was 1500mg / L, and the operation mode of the accumulation cycle was water inlet→aeration+stirring→precipitation→water outlet, and the operation cycle was 12h. The influent water is wood flour hydrolyzate, the carbon source concentration of the water is 500mg / L, the nitrogen source concentration is 40mg / L, and the phosphorus source concentration is 8mg / L, and 100mg / L of MgSO4 and 1ml of trace element solution are added to each 1L of water during water distribution. . After the end of each cycle aeration section, 100ml of activated sludge mixture was discharged. The precipitation section in the running cycle is 1h, and 250ml of supernatant is discharged after the precipitation is completed. Activated sludge was sampled for measurement during the ninth incubation cycle. After the mixture was centrifuged, the precipitate was taken and freeze-dried...

Embodiment 2

[0045] The acclimated sewage was added into the beaker, the initial mixed liquid suspended particle concentration (MLSS) of the sewage was 1500mg / L, and the operation mode of the accumulation cycle was water inlet→aeration+stirring→precipitation→water outlet, and the operation cycle was 12h. The influent water is wood flour hydrolyzate, the carbon source concentration of the water is 800mg / L, the nitrogen source concentration is 40mg / L, and the phosphorus source concentration is 8mg / L, and 100mg / L of MgSO4 and 1ml of trace element solution are added to each 1L of water during water distribution. . After the end of each cycle aeration section, 100ml of activated sludge mixture was discharged. The precipitation section in the running cycle is 1h, and 250ml of supernatant is discharged after the precipitation is completed. Activated sludge was sampled for measurement during the ninth incubation cycle. After the mixture was centrifuged, the precipitate was taken and freeze-dried...

Embodiment 3

[0047] The acclimated sewage was added into the beaker, the initial mixed liquid suspended particle concentration (MLSS) of the sewage was 1500mg / L, and the operation mode of the accumulation cycle was water inlet→aeration+stirring→precipitation→water outlet, and the operation cycle was 12h. The influent water is wood flour hydrolyzate, the carbon source concentration of the water is 1100mg / L, the nitrogen source concentration is 40mg / L, and the phosphorus source concentration is 8mg / L, and 100mg / L of MgSO4 and 1ml of trace element solution are added to each 1L of water during water distribution. . After the end of each cycle aeration section, 100ml of activated sludge mixture was discharged. The precipitation section in the running cycle is 1h, and 250ml of supernatant is discharged after the precipitation is completed. Activated sludge was sampled for measurement during the ninth incubation cycle. After the mixture was centrifuged, the precipitate was taken and freeze-drie...

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Abstract

The invention relates to a method for synthesizing poly-3-hydroxybutyrate / 3-polyhydroxybutyrate (PHBV) from wooden carbon source domesticated sewage mixed with bacteria. The method comprises the following steps: (1) carrying out hydrolysis and enzymolysis on a wooden biological resource by using a hydrothermal pretreatment method; (2) by taking the hydrolysis and enzymolysis product as a carbon source, domesticating sewage with activated sludge by using an aerobiotic dynamic material supply method, and enriching bacteria capable of synthesizing polyhydroxyalkanoate (PHA); (3) accumulating PHAby controlling an unbalanced nutrition environment, and increasing the yield of PHA, thereby finally confirming that the accumulated PHA is PHBV. With the comparison between dry cell weights of different experiment groups and data of PHBV yields, optimal culture conditions can be acquired. After 9 accumulation cycles of the domesticated activated sludge, 303.7-1084.4 mg / of PHBV can be produced, PHB accounts for 40.45-69.14%, and PHV accounts for 30.86-59.55%; finally, at a pH value of 7 and a total sugar concentration of 1700 mg / , the yield of the PHBV meets the maximum value under a condition of sufficient inorganic salt components and oxygen.

Description

technical field [0001] The invention belongs to the fields of efficient and high-value utilization of biomass resources, biodegradable plastics and solid waste treatment, relates to a method for synthesizing PHBV by utilizing woody carbon source domesticated sewage mixed bacteria, and in particular relates to a woody carbon source domesticated sewage mixed bacteria A method for the synthesis of poly-3-hydroxybutyrate / 3-hydroxyvalerate copolymer (PHBV). Background technique [0002] In recent years, with the development of the petroleum industry, more and more chemical synthetic plastics have been used in people's lives, resulting in a lot of non-degradable plastic waste, resulting in white pollution, leading to many serious problems. Therefore, efforts are being made to find a product that can replace petroleum-based plastics. PHBV is an environment-friendly polymer bioplastic, which has similar material properties to traditional petroleum-based plastics such as polyethylen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/62C02F3/34C12R1/01C12R1/07C12R1/185C12R1/645C12R1/38
CPCC02F3/34C02F3/347C12P7/625C12P39/00
Inventor 马晓军尹芬李冬娜
Owner TIANJIN UNIV OF SCI & TECH
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