165 results about "3-Hydroxybutanoate" patented technology

Several novel PHA polymer compositions produced using biological systems include monomers such as 3-hydroxybutyrate, 3-hydroxypropionate, 2-hydroxybutyrate, 3-hydroxyvalerate, 4-hydroxybutyrate, 4-hydroxyvalerate and 5-hydroxyvalerate. These PHA compositions can readily be extended to incorporate additional monomers including, for example, 3-hydroxyhexanoate, 4-hydroxyhexanoate, 6-hydroxyhexanoate or other longer chain 3-hydroxyacids containing seven or more carbons. This can be accomplished by taking natural PHA producers and mutating through chemical or transposon mutagenesis to delete or inactivate genes encoding undesirable activities. Alternatively, the strains can be genetically engineered to express only those enzymes required for the production of the desired polymer composition. Methods for genetically engineering PHA producing microbes are widely known in the art (Huisman and Madison, 1998, Microbiology and Molecular Biology Reviews, 63: 21-53). These polymers have a variety of uses in medical, industrial and other commercial areas.

The present invention provides a card base which is degradable by microbes in natural environment. The card base is excellent in properties necessary for card bases, such as tensile strength, impact strength, flex temperature, heat resistance, resistance to thermal expansion and contraction, blocking resistance and humidity resistance, and has rigidity, bending resistance and durability. The card base contains, as essential components, a 3-hydroxybutylate/3-hydroxyvalerate copolymer and a lactic acid polymer, and, where necessary, a polycaprolactone or a high-molecular aliphatic polyester. The card base has a single-layer structure, or a sandwich structure further having overlay layers comprising a composition containing, as essential components, a lactic acid polymer and either or both of a polycaprolactone and a high-molecular alphatic polyester.

Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is E. coli. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).

The present invention relates to fibers, and nonwovens comprising said fibers, comprising a biodegradable copolymer, wherein the copolymer comprises at least two randomly repeating monomer units (RRMU) wherein the first RRMU monomer unit has the structure and the second RRMU has the structure wherein at least 50% of the RRMUs have the structure of the first RRMU. The present invention further relates to an absorbent article comprising a liquid previous topsheet, a biodegradable liquid impervious backsheet comprising the above fibers and/or nonwovens, and an absorbent core positioned between the topsheet and the backsheet.

Compositions of polymer blends of polyvinylchloride (PVC), a low molecular weight plasticizer and polyhydroxyalkanoate (PHA) are described. In certain embodiments, the PHA is a poly-3-hydroxybutyrate-co-4-hydroxybutyrate copolymer having a weight percent 4-hydroxybutyrate of 30-45%. In other embodiments the PHA is a multiphase P3HB-4HB copolymer blend having one phase fully amorphous. The PHA is mixed with the PVC and low molecular weight plasticizer to optimize the PVC's optical, thermal and mechanical properties. In certain embodiments, the polymer is branched with optionally additives that improve properties. Methods of making the compositions of the invention are also described. The invention also includes articles, films and laminates comprising the compositions.

The present invention relates to a method for producing optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid esters wherein a 2-(N-substituted aminomethyl)-3-oxobutyric acid ester is treated with an enzyme source capable of stereoselectively reducing said ester to the corresponding optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid ester having the (2S,3R) configuration. The present invention provides an efficient method for industrially producing optically active 2-(N-substituted aminomethyl)-3-hydroxybutyric acid esters, in particular such compounds having the (2S,3R) configuration, which are useful as intermediates for the production of medicinal compounds, among others.

The present invention relates to fibers, and nonwovens comprising said fibers, comprising a biodegradable copolymer, wherein the copolymer comprises at least two randomly repeating monomer units (RRMU) wherein the first RRMU monomer unit has the structure and the second RRMU has the structure wherein at least 50% of the RRMUs have the structure of the first RRMU. The present invention further relates to an absorbent article comprising a liquid pervious topsheet, a biodegradable liquid impervious backsheet comprising the above fibers and/or nonwovens, and an absorbent core positioned between the topsheet and the backsheet.

The invention provides a preparation method for (R)-4-hydroxy-2-oxo-1-pyrrolidine acetamide. The preparation method comprises the following steps: (1) with R-4-chloro-3-hydroxy butyrate as an initial raw material, subjecting the initial raw material and an azido reaction agent to an azido reaction so as to obtain an intermediate I; (2) subjecting the intermediate I to a reduction reaction so as to obtain an intermediate II; (3) subjecting the intermediate II and halogenated acetate to a condensation reaction so as to obtain an intermediate III; (4) subjecting the intermediate III to a ring closure reaction so as to obtain an intermediate IV; and (5) subjecting the intermediate IV to an aminolysis reaction so as to obtain (R)-4-hydroxy-2-oxo-1-pyrrolidine acetamide. The preparation method can prepare a (R)-4-hydroxy-2-oxo-1-pyrrolidine acetamide product with ideal yield of at least more than 38%, and a novel synthetic route is opened up for (R)-4-hydroxy-2-oxo-1-pyrrolidine acetamide.

Methods are provided for producing biodegradable polyhydroxyalkanoates (PHAs) with desired geometry, molecular mass, mechanical and/or physical-chemical properties from glycerol, an inexpensive carbon source and byproduct of the biodiesel industry. Microorganisms capable of converting carbon to PHA can be used to convert biodiesel-glycerol to poly-3-hydroxybutyrate (PHB) or other monomer or copolymer PHAs via fermentation. The microorganisms are cultured in a medium comprising glycerol as a primary carbon source and one or more low molecular mass organic acids as a secondary carbon source. Biomass can be harvested from the culture medium and crude PHA extracted and purified, thereby recovering purified PHA with the desired property. After PHA isolation, a nucleating agent can be added to improve certain physical-chemical properties of the PHA; e.g., crystallization temperature, to enhance performance of the PHA during injection molding.

The invention discloses resource-renewable and biodegradable conductive fiber and a preparation method thereof. The fiber consists of the following raw materials in parts by weight: 30-70 parts of polylactic acid (PLA), 30-70 parts of poly(3-hydroxybutyric acid-co-3-hydroxyvalerate (PHBV) and 0.05-8 parts of conductive filler. The preparation method of the conductive fiber comprises the following steps of: (1) proportionally pre-mixing PHBV (if PLA is not less than 50 parts) or PLA (if PLA is less than 50 parts) and the conductive filler, and performing melt blending and granulation to obtain the conductive master batch of the PHBV or PLA; (2) proportionally pre-mixing the PLA or PHBV and the conductive master batch of the PHBV or PLA, and performing melt blending and granulation to obtain composite conductive mater batches; and (3) spinning and drafting the composite conductive master batches one by one to obtain the conductive fiber. The conductive fiber disclosed by the invention can be used as the material for electrodes, static resistance, low-temperature heating, electromagnetic shielding, thermal sensitivity, gas sensitivity and the like.

The invention relates to a method for preparing a high-toughness degradable material by using a melt-grafting blending method, which comprises the following steps: (1) carrying out melt-grafting on polycaprolactone PCL, anhydride and an initiator, and then obtaining modified polycaprolactone by way of extrusion granulation, wherein the mass ratio of the polycaprolactone PCL to the anhydride to the initiator is 100: (1 to 10): 0.5; and (2) carrying out melt-grafting blending on poly (3-hydroxybutyrate-3-hydroxyvalerate) PHBV, the modified polycaprolactone, methyl acrylate and an initiator, and then obtaining the high-toughness PHBV-class degradable material by way of extrusion granulation, wherein the mass ratio of the poly (3-hydroxybutyrate-3-hydroxyvalerate) PHBV to the modified polycaprolactone to the methyl acrylate to the initiator is 100: (5 to 50): (0.5 to 10):0.5, the processing temperature is 100 to 170 DEG C, and the screw speed is 50-80 R.P.M. The method is simple in process, low in cost, and suitable for industrialization, and the elongation at break of the produced PHBV-class degradable material can reach 560 percent; therefore, the method of the invention has good application prospect.

The invention discloses a method for preparing hydroxyalkanoate homopolymer and special bacteria thereof. Recombinant pseudomonas putida is prepared by the method comprising the following steps of: depriving coding functions of 3-hydroxyfatty acyl-coenzyme A dehydrogenase coding gene and 3-ketoacyl coenzyme A thiolase coding gene in pseudomonas putida to obtain recombinant pseudomonas putida, and recording the recombinant pseudomonas putida as recombinant pseudomonas putida I. Experiments prove that the hydroxyalkanoate homopolymer, particularly medium and long-chain hydroxyalkanoate homopolymer (C4-C14), can be obtained by using the recombinant bacteria organism to synthesize polyhydroxyalkanoate. The obtained hydroxyalkanoate homopolymer has high purity and high yield. The method of the invention can synthesize the medium and long-chain hydroxyalkanoate homopolymer with random length, and overcomes the defect that only one 3-hydroxybutyrate (HB) homopolymer can be obtained by biosynthesis in the prior art. Therefore, the method of the invention has broad application prospect in the biosynthesis field of the hydroxyalkanoate homopolymer.

Compositions comprising recyclate blends of polymer recyclate and polyester additives are described. In certain embodiments, the polyester additive is a PHA such as a poly-3-hydroxybutyrate-co-4-hydroxybutyrate copolymer having a weight percent 4-hydroxybutyrate of 8.5-45%. In other embodiments the PHA is mixed with the recyclate polymer at concentrations of 10-50% by weight of the recyclate blend. Methods of making the compositions of the invention are also described. The invention also includes articles made from the recyclate blends.

The invention discloses a recombinant carbonyl reductase derived from Burkholderia gladioli ZJB-12126 and a coding gene thereof, a recombinant vector containing the gene, a recombinant gene engineering bacterium converted from the recombinant vector and application in preparing the recombinant carbonyl reductase.2-benzoylaminomethyl-3-one butyrate, N,N-bis-methyl-3-one-3-(2-thienyl)propanamide, ethyl 4-chloroacetoacetate (COBE) and tert-butyl (R)-6-cyano-5-hydroxy-3-carbonylhexanoate used as substrates are subjected to biological catalytic reaction to prepare high-optical-purity (2S,3R)-2-benzamidomethyl-3-hydroxybutyrate, (S)-N,N-bis-methyl-3-hydroxy-3-(2-thienyl)propanamide, ethyl (S)-4-chloro-3-hydroxybutyrate and tert-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate.

The invention provides Burkholderia gladioli ZIB-12126, which is a new bacterial strain capable of catalyzing asymmetrical reduction of carbonyl, and application of the bacterial strain in preparation of (2S,3R)-2-benzoyl aminomethyl-3-hydroxybutyric acid ester by racemizing 2-benzoyl aminomethyl-3-hydroxybutyric acid ester in a microbial conversion mode. The bacterial strain is preserved in the Chinese Typical Culture Collection Center with an address of Wuhan College, Wuhan, China and the zip code of 430072, the preservation serial number of CCTCC No. M 2012379 on September 255th, 2012. The bacterial strain provided by the invention is used for synthesizing the (2S,3R)-2-benzoyl aminomethyl-3-hydroxybutyric acid ester through biologic conversion, with gentle reaction condition and environmental-friendliness; and more importantly, the product configuration is mainly (2S,3R) configuration, and step of turning the product configuration by using a chemical method is not needed, so that the operation process is simple and has good industrial application prospects.

Compositions of polymer blends of polyvinylchloride (PVC) or polymethylmethacrylate (PMMA) or polyoxymethylene (POM) and polyhydroxyalkanoate (PHA) are described. In certain embodiments, the PHA is a poly-3-hydroxybutyrate-co-4-hydroxybutyrate copolymer having a weight percent 4-hydroxybutyrate of 30-45%. In other embodiments the PHA is a multiphase P3HB-4HB copolymer blend having one phase fully amorphous. The PHA is mixed with the PVC or PMMA or POM to optimize its optical, thermal and mechanical properties. In certain embodiments, the polymer is branched with optionally additives that improve properties. Methods of making the compositions of the invention are also described. The invention also includes articles, films and laminates comprising the compositions.

The invention provides a polylactic acid and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) nano modified composite. The molar content of 4-hydroxybutyrate in the poly(3-hydroxybutyrate-co-4-hydroxybutyrate) is 15%-35%. A preparation method of the composite includes: firstly, compounding polylactic acid, poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and filler or auxiliaries according to a stated proportion, adding into a high-speed mixer for mixing 5-8min to obtain premixed matrix; secondly, using a double-screw extruder for mixing extrusion of the premixed matrix to obtain material strips, wherein set parameters of double screws include that the main unit frequency is 10Hz, the feeding frequency is 8Hz, eight segments are arranged from a feeding port A to a head, and temperature set values of the eight segments are 0 DEG C, 150 DEG C, 160 DEG C, 170 DEG C, 180 DEG C, 180 DEG C, 180 DEG C and 180 DEG C respectively, and temperature set parameters of a feed cylinder of an injection molding machine include that HZ, H1, H2 and H3 are 170 DEG C and H4 and H6 are 0 DEG C or heating-free; and thirdly, subjecting the material strips to cooling and granulating after extrusion to obtain the polylactic acid and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) nano modified composite.

The invention provides a poly(3-hydroxybutyrate-co-4-hydroxybutyrate) foaming material and a preparation method thereof, and relates to the field of foaming materials. The foaming material is prepared from the following materials in parts by weight: 85-100 parts of P34HB, 0.5-15 parts of a crosslinking agent, 1-10 parts of a foaming agent, 0.1-15 parts of a foaming aid, 0-10 parts of a cell nucleating agent and 0-20 parts of a foaming stabilizer; and the foaming material is obtained by sequentially performing drying, raw material blending, melt blending, open milling sheeting and mold pressing foaming molding. The foaming ratio of the foaming material prepared by the method provided by the invention can reach 56.69%, the mechanical properties are significantly improved, the impact strength is improved by 74.36% compared with that of an unfoamed sample, and the tensile strength is more than or equal to 5.81MPa, the cost is reduced, the obtained foaming material is biodegradable, has excellent comprehensive performance, is simple in processing technology, and can widely replace a polystyrene foaming material, and then the application range of a biodegradable material P34HB can be enlarged.

The invention provides a preparation method of 4-hydroxyl ketopyrrolidine-2-acetamide as a medicine with a cerebral metabolism improving function, comprising the following steps of: 1, reducing 4-halogenated acetylacetic ester as a raw material into 4-chloro-3hydroxybutyrate by using a reducing agent; and 2, condensing the obtained 4-chloro-3-hydroxybutyrate of the step 1 and glycine amide or glycine amide hydrochloride to obtain 4-hydroxyl ketopyrrolidine-2-acetamide and purifying the obtained 4-hydroxyl ketopyrrolidine-2-acetamide. The invention ensures that the total yield of a product is increased above 36 percent, shortens a process route and reaction time, reduces an intermediate process and is more suitable for the large-scale production of medical industry.