4 results about "Nitrile hydratase activity" patented technology

The present invention provides methods for the manufacture of amides from their corresponding nitriles using a biocatalyst and purification of the manufactured amides using membrane filtration. The invention also relates to methods of using membrane filtration for isolating and reusing biocatalysts. More specifically, the inventive method produces a crude aqueous acrylamide (AMD) by feeding acrylonitrile (AN), water, and a biocatalyst with nitrile hydratase activity into a reactor. After complete conversion of AN to AMD, the crude reaction is directly purified by membrane filtration to produce a purified AMD monomer (without nitrile hydratase activity) and a recovered biocatalyst with nitrile hydratase activity. Purified monomer (e.g., AMD without enzyme activity) in the permeate may be stored, transported, and used for polymerization. Biocatalyst in the retentate retains enzymatic activity and may be reused.

Provided is a method for producing an amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity, the method being characterized in that the amide compound is produced from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity, at least in the case of a liquid composition that becomes a reaction liquid. When the concentration of the nitrile compound in the gas phase part of the reaction tank containing the reaction liquid in the presence of air is within the explosion range, the hydration reaction is carried out under the condition that the oxygen concentration in the gas phase part of the reaction tank is 10 vol% or less. The nitrile compound is preferably, for example, acrylonitrile or methacrylonitrile.

A method for producing an amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity includes, at least in the presence of air, when a liquid composition of a reaction solution is such that a concentration of the nitrile compound in a gas phase portion of a reactor containing the reaction solution is in an explosion range, performing a hydration reaction under a condition that an oxygen concentration of the gas phase portion of the reactor is 10% by volume or less. The nitrile compound is, for example, preferably acrylonitrile or methacrylonitrile.

The present invention pertains to a method for producing an amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity. An amide compound is efficiently produced using this method by suppressing a decrease in the biocatalyst activity and improving the reaction rate at which the nitrile compound is converted to the amide compound.