7 results about "Nitrile hydratase activity" patented technology

The present invention provides methods for the manufacture of amides from their corresponding nitriles using a biocatalyst and purification of the manufactured amides using membrane filtration. The invention also relates to methods of using membrane filtration for isolating and reusing biocatalysts. More specifically, the inventive method produces a crude aqueous acrylamide (AMD) by feeding acrylonitrile (AN), water, and a biocatalyst with nitrile hydratase activity into a reactor. After complete conversion of AN to AMD, the crude reaction is directly purified by membrane filtration to produce a purified AMD monomer (without nitrile hydratase activity) and a recovered biocatalyst with nitrile hydratase activity. Purified monomer (e.g., AMD without enzyme activity) in the permeate may be stored, transported, and used for polymerization. Biocatalyst in the retentate retains enzymatic activity and may be reused.

Provided is a method for producing an amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity, the method being characterized in that the amide compound is produced from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity, at least in the case of a liquid composition that becomes a reaction liquid. When the concentration of the nitrile compound in the gas phase part of the reaction tank containing the reaction liquid in the presence of air is within the explosion range, the hydration reaction is carried out under the condition that the oxygen concentration in the gas phase part of the reaction tank is 10 vol% or less. The nitrile compound is preferably, for example, acrylonitrile or methacrylonitrile.

Provided is a method for efficiently producing an amide compound from a nitrile compound using a biocatalyst, and for producing an amide compound aqueous solution having low foamability. In a method for producing an amide compound by subjecting a nitrile compound to a hydration reaction in the presence of a biocatalyst having nitrile hydratase activity, the pH of the reaction liquid is increased during the hydration reaction. In one example, a hydration reaction is carried out by setting the pH of the reaction liquid in a second half of a reaction in which the content of the amide compound is 40 mass% or more higher than the pH of the reaction liquid in a first half of the reaction in which the content of the amide compound is less than 40 mass% with respect to the total mass of the reaction liquid.

A method for producing an amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity includes, at least in the presence of air, when a liquid composition of a reaction solution is such that a concentration of the nitrile compound in a gas phase portion of a reactor containing the reaction solution is in an explosion range, performing a hydration reaction under a condition that an oxygen concentration of the gas phase portion of the reactor is 10% by volume or less. The nitrile compound is, for example, preferably acrylonitrile or methacrylonitrile.

To provide an improved method for producing an acrylamide aqueous solution.SOLUTION: A method for producing acrylamide aqueous solution includes the steps of: (a) combining water and at least one biocatalyst having nitrile hydratase activity to provide slurry; (b) feeding acrylonitrile into a reactor including the slurry to provide reaction mixture; (c) monitoring the reaction mixture by online GC to measure a concentration of acrylonitrile in a headspace of the reactor by using several detection techniques selected from the group consisting of a hydrogen flame ionization detector, mass spectrometry, a thermal conductivity detector, an electron capture detector, a nitrogen-phosphorus detector and vacuum ultraviolet detector.SELECTED DRAWING: None

The present invention pertains to a method for producing an amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity. An amide compound is efficiently produced using this method by suppressing a decrease in the biocatalyst activity and improving the reaction rate at which the nitrile compound is converted to the amide compound.

The invention concerns a method for preparing a compound of formula (I) and its use for producing methionine and analogs thereof wherein formula (I) is (I) in which X is selected from S and Se; R1 is selected from H, alkyl groups, aryl groups and alkylaryl groups; R2 is selected from OH, NR5R6 where R5 and R6 are identical or different and are selected from H and alkyl groups; and R3 is C(O)NR7R8 where R7 and R8 are identical or different and are selected from H and alkyl groups; which method comprising the reaction of a compound of formula (II) in which X, R1 and R2 are as defined above for the compound of formula (I); and R4 is CN; in the presence of an enzyme having a nitrile hydratase activity and comprising at least an α-subunit and a β-subunit, said α-subunit being defined by SEQ ID No: 1 and said β-subunit being defined by SEQ ID No: 2, wherein said α-subunit has at least one mutation selected from the group consisting of Leu6, Ile13, Ala19, Thr36, Asp40, Ala43, Asn48, Arg71, Asp92, Met94, Phe126, Gly148 and Val204, and / or said β-subunit has at least one mutation selected from the group consisting of Val4, Thr10, Val24, Phe37, Phe41, Met46, Leu48, Phe51, His79, Gln96, Pro107, Glu108, Phe118, Leu127, Arg160, Leu186, Ala200, Gly205, Pro206, Ser212, Tyr215, Ala230 and Ala231, said amino acid in said position(s) being replaced by anyone of alanine, arginine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, or valine.