Method for preparing 2,2-dimethyl cyclopropanecarboxamide and bacterial strain thereof by biological catalysis

Abstract

The invention provides a Rhodococcus boritolerans FW815 enriched in soil and sewage and sieving high nitrile hydratase active mircoorganism strain, and a process that applies the strain to biological catalysis methods for the preparation of 2,2-dimethyl cyclopropane formamide. The 2,2-dimethyl cyclopropane formamide is prepared by converting 2,2-dimethyl cyclopropane formonitrile via the nitrile hydratase generated by the strain, wherein, the reaction medium is water, constituents of the reaction system are simple, the biological catalysis efficiency is high, the conversion time is short, theconversion rate for substrates is high, the yield of the 2,2-dimethyl cyclopropane formamide product is high and the extraction and purification processes are simple.

Description

(1) Technical field

[0001] The invention relates to a bacterial strain with high nitrile hydratase activity and its application in biocatalytic preparation of 2,2-dimethylcyclopropanamide. (2) Background technology

[0002] Nitriles are a class of compounds containing cyano functional groups, which play a very important role in organic synthesis. The cyano group is an important source of C1, which is usually introduced into organic matter as a "water-stable carbanion" to form amines, imines, amides, amidines, carboxylic acids, and carbonyl compounds, etc. However, the traditional chemical conversion of nitriles has the disadvantages of high energy consumption, harsh reaction conditions, strong acid or strong base, high temperature and metal catalysts, low reaction selectivity, and the formation of a large number of by-products. Nitrile compounds also exist in the natural environment and in bacteria, fungi, plants, and animals. In biological systems, known nitrile hydratases...

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