Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Transport method of microorganisms

A technology of microbial bacteria and containers, applied in the field of transportation of microbial bacteria, can solve problems such as reduction and loss of microbial enzyme catalytic ability, and achieve the effect of reducing cooling costs

Active Publication Date: 2016-06-01
MITSUBISHI CHEM CORP
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is, the activity of the enzyme must be maintained to avoid the loss or reduction of the catalytic ability of the microbial enzyme due to the mixing, corruption, or lysis of bacteria during storage or transportation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Transport method of microorganisms
  • Transport method of microorganisms
  • Transport method of microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 and 2、 comparative example 1

[0038] to cultivate

[0039] Rhodococcus rhodochrous J-1 strain (Rhodococcus rhodochrous J-1: FERMBP-1478) having nitrile hydratase activity was used to contain 2% by mass of glucose, 1% by mass of urea, 0.5% by mass of peptone, 0.3% of yeast extract, and 0.05% of cobalt chloride. Mass % medium (pH 7.0) was used for aerobic culture.

[0040] Preparation of bacterial cell suspension for preservation

[0041] The cultured microbial cells were recovered by centrifugation (12000 rpm, 20 minutes), and washed with a 0.1% sodium acrylate aqueous solution (pH 7.0). The above-mentioned aqueous solution was added to the washed bacterial cells to obtain a bacterial cell suspension (10% by mass as dry bacterial cells).

[0042] Preservation of bacterial suspension

[0043] Add 115mL (Example 1) and 70mL (Comparative Example 1) of the bacterial cell suspension prepared by the above method into a polyethylene container with a total volume of 120mL, seal it, and shake it at 30°C and 120rp...

Embodiment 3

[0048] Embodiment 3, comparative example 2

[0049] Preparation of transformant with nitrile hydratase derived from Rhodococcus rhodochrous strain M8

[0050] (1) Preparation of chromosomal DNA of Rhodococcus rhodochrous M8 strain (hereinafter referred to as M8 strain.)

[0051] The M8 strain (SU1731814) can be obtained from the Russian strain center IBFM (VKPMS-926).

[0052] M8 strain was dissolved in 100mL of MYK (0.5% polypeptone, 0.3% Bacto yeast extract, 0.3% Bacto malt extract, 0.2% K 2 HPO 4 , 0.2%KH 2 PO 4 ) medium (pH 7.0), cultured with shaking at 30°C for 72 hours. Centrifuge the culture medium, and suspend the collected cells in 4 mL of Saline-EDTA solution (0.1 MEDTA, 0.15 M NaCl (pH 8.0)). 8 mg of lysozyme was added to the suspension, shaken at 37°C for 1 to 2 hours, and then frozen at -20°C.

[0053] Next, 10 mL of a Tris-SDS solution (1% SDS, 0.1M NaCl, 0.1M Tris-HCl (pH 9.0)) was added to the suspension while shaking it steadily. Further, proteina...

Embodiment 4、 comparative example 3

[0090] to cultivate

[0091] Pseudomonas marginalis DSM16275 strain (Pseudomonas marginalis DSM16275) having nitrile hydratase activity was used to contain 0.2% by mass of glycerin, 0.05% by mass of citric acid, 0.15% of yeast extract, 0.3% by mass of potassium dihydrogenphosphate, and 0.7% by mass of dipotassium hydrogenphosphate. %, 0.0004% by mass of iron sulfate, 0.01% by mass of manganese sulfate, 1.25% by mass of methylurea, and 0.001% by mass of cobalt chloride (pH 7.0) for aerobic culture.

[0092] Preparation of bacterial cell suspension for preservation

[0093] The cultured microbial cells were recovered by centrifugation (12000 rpm, 20 minutes), and washed with a 0.1% sodium acrylate aqueous solution (pH 7.0). The above-mentioned aqueous solution was added to the washed bacterial cells to obtain a bacterial cell suspension (10% by mass as dry bacterial cells).

[0094] 100 mL (Example 4) and 80 mL (Comparative Example 3) of the bacterial cell suspension prepared ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

A method for storing microbial cells having nitrile hydratase activity in a hermetically sealable container, and a method for transporting microbial cells having nitrile hydratase using the container. The methods are characterized in that the vapor phase portion within the container is set to 2-20% (inclusive) of the total interior volume of the container, said vapor phase portion being filled with a suspension that contains the microbial cells.

Description

technical field [0001] The present invention relates to a kind of method, it is when the microbial thallus cell that has nitrile hydratase activity is filled in airtight container and is transported, by making the gas phase part inside the airtight container account for 20% or less of the total volume, thereby make nitrile hydratase The activity is maintained in a stable state for transport. Background technique [0002] Enzymes produced by microorganisms are used in various situations as catalysts for chemical transformation reactions. In particular, amides, carboxylic acids, α-hydroxycarboxylic acids, etc. which are important in the chemical industry can be produced inexpensively by using nitrile hydratase, nitrilase, etc., which have the ability to hydrate or hydrolyze nitrile groups. Furthermore, by using the above-mentioned enzymes having optically specific hydration or optically specific hydrolysis ability, it becomes possible to produce optically active carboxylic ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/78C12R1/01
CPCC12N9/96C12N1/04C12N9/78C12N9/88C12Y305/05001C12Y402/01084
Inventor 竹内雅人渡边文昭
Owner MITSUBISHI CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com