Halomonas taeanensis for degradation of phenol

A Halomonas, phenol technology, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., to achieve good salt resistance effect, good economic value and application prospects

Active Publication Date: 2017-09-15
INNER MONGOLIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are studies on the degradation characteristics of pollutants under normal conditions by Halomonas, but there are no relevant reports on the degradation of pollutants under high-salt conditions, especially the degradation characteristics of phenolic substances

Method used

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  • Halomonas taeanensis for degradation of phenol
  • Halomonas taeanensis for degradation of phenol
  • Halomonas taeanensis for degradation of phenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The isolation and identification of embodiment 1 Halomonas H17

[0024] 1. Isolation of Halomonas H17

[0025] a. Dissolve 1-5g of collected solid samples (salt lake sludge, submarine sludge, high-salt wastewater treatment sludge, etc.) or 500ml of liquid samples (salt lake water, seawater, high-salt wastewater, etc.) in 10-50ml Liquid medium (10g MgSO 4 ·7H 2 O, 0.2g CaCl 2 2H 2 O, 2-5g KCl, 2.5g tryptone, 10g yeast extract, NaCl15%, distilled water were added to 1000ml. Adjust the pH to about 7.2, sterilize at 121°C for 15 minutes), shake at 80-160 rpm for 30-120 minutes at room temperature, take it out and let it stand for 20-40 minutes, draw 100 μL with a pipette, and spread evenly on the solid medium for culture Place on a flat plate (obtained by adding 2% agar to the above-mentioned liquid medium), and place it in a constant temperature incubator at 28°C for static culture.

[0026] b. When visible colonies are grown, use an inoculation loop to pick a single...

Embodiment 2

[0036] The performance test of the degradation phenol of embodiment 2 Halomonas H17

[0037] The degradation of phenol in the medium by Halomonas H17 was detected by high performance liquid chromatography.

[0038] 1. Growth of strain H17 and characteristics of phenol degradation

[0039] Bacterial strain H17 is 200mg / L in phenol concentration, and the medium that sodium chloride concentration is 10%, the formula of described medium 1L is 10g MgSO 4 ·7H 2 O, 0.2g CaCl 2 2H 2 O, 2-5g KCl, 2.5g tryptone, 10g yeast extract, NaCl10%, distilled water were added to 1000ml. Adjust the pH to about 7.2, and sterilize at 121°C for 15 minutes.

[0040] The curves of strain growth and phenol concentration changes over time in the medium are as follows: figure 2shown. Strain H17 entered the logarithmic growth phase after a stagnation period of about 8 hours. During this period, the strain grew almost linearly with time, and the strain was in a stable growth period near 30 hours and...

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Abstract

The invention provides a strain of Halomonas taeanensis and an application thereof. The bacterial strain is named H17 and is assigned the accession number CCTCC No: M2016306. The bacterial strain can degrade phenol in a liquid culture medium containing 200 mg / L of phenol within 50 h by more than 90%. 1L of the liquid culture medium includes 10 g of MgSO4.7H2O, 0.2 g of CaCl2.2H2O, 2-5 g of KCl, 2.5 g of tryptone, 10 g of yeast extract, 10% of NaCl, and distilled water added to 1000 ml, wherein pH value is regulated to about 7.2 and sterilization is carried out at 121 DEG C for 15 min. The Halomonas taeanensis H17 has strong capability of degrading phenol, can be used for biological recovery of phenol polluted environments and has excellent economic value and application prospect.

Description

technical field [0001] The invention relates to the technical field of microorganisms and biodegradation, in particular to a halomonas and its application in degrading phenol. Background technique [0002] Phenol and its derivatives are highly toxic to humans, animals and plants, and have been included in the list of environmental priority pollutants. Phenolic wastewater mainly comes from industries such as papermaking, oil refining, synthetic fiber, synthetic rubber, and pesticides, and is the main pollutant in industrial wastewater. In addition to phenolic pollutants, these phenolic wastewater often contain a large amount of salt, making it a high-salt phenolic wastewater, which will cause serious pollution to soil, surface water and groundwater when discharged into the environment. Due to the inhibitory and toxic effects of high-salt environment on the growth of microorganisms, conventional biodegradation technology has a bottleneck problem in the treatment of such waste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01C02F101/34A62D101/28
CPCA62D3/02A62D2101/28C02F3/34C02F2101/34C12N1/02C12N1/20C12N1/205C12R2001/01C12R2001/44
Inventor 许继飞赵吉宋晓雪武琳慧刘崎峰王川包智华
Owner INNER MONGOLIA UNIVERSITY
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