Composite flora for regulating and controlling prawn culture water body

A technology of complex flora and prawns, applied in the direction of microorganism-based methods, applications, bacteria, etc., can solve the problem of no uniform standard for safety evaluation, achieve the effect of improving the ability of resisting pathogenic Vibrio and improving the treatment effect

Active Publication Date: 2021-04-30
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no unified standard for safety evaluation, which mainly includes in vitro safety evaluation and in vivo safety evaluation of probiotics for hemolysis, virulence factors, antibiotic resistance and toxic metabolite detection.

Method used

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  • Composite flora for regulating and controlling prawn culture water body
  • Composite flora for regulating and controlling prawn culture water body
  • Composite flora for regulating and controlling prawn culture water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening for Halomonas alkalophilus AC3-2 strain, Halomonas rugeria BD15-5 strain and Halomonas beautifula BD15-8

[0026] 1) Screening sample source and screening medium

[0027] In September 2017, the shrimp biofloc culture system obtained from Weihai Wendeng Taiyu Aquatic Breeding Co., Ltd. was sampled and analyzed (salinity was 30‰). The biofloc was made of bagasse, rice husk powder and Bacillus as components , which is cultivated by the floc generation device in the culture system, can effectively provide the water treatment effect of cultured prawns, and the prawns will not produce stress response.

[0028] Under sterile conditions, take 0.2 mL and spread it on a solid LB plate, store it at 4°C, and bring it back to the laboratory.

[0029] The composition ratio of the medium used in the screening process is as follows:

[0030] Enrichment medium: NaNO 2 1g, CH 3 COONa 4.8g, filtered seawater 1L, pH 7.5;

[0031] Screening medium: NaNO 2 1g, CH...

Embodiment 2

[0049] Embodiment 2: the physicochemical property of bacterial strain

[0050] Strain AC3-2 Gram-negative, non-spore-shaped, rod-shaped, fusiform, straight or curved. After the LB solid medium was cultured in a constant temperature incubator at 28°C for 24 hours, the appearance of the colony was as follows: the colony was a small round shape, milky yellow opaque, with neat edges and a smooth surface ( figure 2 ) strain's growth temperature range is 20240°C, the optimum temperature is 37°C; the growth pH range is 729, the optimum pH value is 8;

[0051] Strain BD15-8 Gram-negative, non-spore-shaped, rod-shaped, fusiform, straight or curved. After the LB solid medium was cultured in a constant temperature incubator at 28°C for 24 hours, the appearance of the colony was as follows: the colony was round, with a raised center, neat edges, smooth surface, milky yellow opaque ( figure 2 ). The growth temperature range of the strain is 22242°C, the optimum temperature is 37°C; th...

Embodiment 3

[0053] Embodiment 3: the nitrogen-reducing performance of three bacterial strains

[0054] 1) Effect of carbon source on strain growth and degradation performance

[0055] Both AC3-2 and BD15-8 strains can grow with glucose, sucrose, sodium acetate and sodium citrate as the sole carbon source. When sodium acetate was the sole carbon source, the OD of strains AC3-2 and BD15-8 600 When sodium citrate was used as the sole carbon source, strains AC3-2 and BD15-8 had the highest removal rate of nitrite nitrogen (both 100%), the highest removal rate of total nitrogen, and no nitrate nitrogen and total ammonia state Nitrogen accumulation ( image 3 A and image 3 B).

[0056] When the strain BD15-5 used sucrose and sodium citrate as the sole carbon source, the removal rates of total ammoniacal nitrogen were 84.6% and 83.1%, and the removal rates of total nitrogen were 65.4% and 63.7%. The accumulation of hydroxylamine is only 0.7mg / L ( image 3 C). Therefore, sucrose is the opt...

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Abstract

The invention provides a composite flora for regulating and controlling a prawn culture water body. The composite flora comprises a halomonas basophila AC3-2 strain, a Lugeria BD15-5 strain and a Halomonas dahurica BD15-8 strain, and the composite flora also contains strains for inhibiting common vibrios in the prawn culture process. On the basis of screening out strains which are safe to prawns and have an efficient nitrogen reduction effect from a prawn biofloc culture system, the screened strains are compounded to obtain the composite flora disclosed by the invention, so that the synergistic effect among the strains is fully exerted, the treatment effect on a culture water body is improved, and the anti-pathogenic vibrio capability of the cultured object is improved.

Description

technical field [0001] The invention belongs to the technical field of screening probiotics, and in particular relates to a composite flora used for regulation and control of prawn breeding water. Background technique [0002] The colonization of probiotics in the culture system and the formation of dominant flora are the key to the high performance of biofloc. The use of strains with strong environmental adaptability and specific functions to cultivate bioflocs can help improve the stability and efficacy of flocs. In the biofloc culture mode, adding exogenous probiotics can significantly increase the digestive enzyme activity of heterogeneous gibel carp, increase the thickness of intestinal muscular layer and submucosa, and promote the growth of heterogeneous gibel carp. [0003] Exogenous probiotics are often difficult to colonize and form a dominant flora because they are not suitable for a specific breeding environment, while the screened indigenous probiotics with spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K50/80A23K10/18A01K61/59C02F3/34C02F101/16C02F103/20C12R1/01C12R1/07
CPCC12N1/20A23K50/80A23K10/18A01K61/59C02F3/34C02F2101/16C02F2101/166C02F2103/20Y02A40/81
Inventor 单洪伟雷柯柯于鹏
Owner OCEAN UNIV OF CHINA
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