Preparation method and application of polyhydroxyalkanoate

A technology of polyhydroxyalkanoate and bacterial strains, which is applied in the field of preparation of polyhydroxyalkanoate, can solve the problems of high COD and BOD, and low conversion rate of polyhydroxyalkanoate, so as to reduce the amount of use and facilitate industrialization The effect of simple realization and preparation method

Active Publication Date: 2022-04-05
MEDPHA CO LTD
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  • Abstract
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  • Application Information

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Problems solved by technology

However, during the preparation of polyhydroxyalkanoate by Halomonas fermentation, a large amount of organic acids will

Method used

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  • Preparation method and application of polyhydroxyalkanoate
  • Preparation method and application of polyhydroxyalkanoate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This example provides a method for preparing polyhydroxyalkanoate, which is obtained by inoculating Halomonas species into a fermentation medium for fermentation. The halomonas species is obtained by activating the halomonas and culturing seeds. The Halomonas was obtained by applying the lake water of Aiding Lake in Qinghai to the separation and purification medium, and culturing it statically at 33° C. for 24 hours.

[0035] The halomonas species is obtained after seed culture is carried out through plate culture medium activation, shake flask seed culture medium and shake flask fermentation culture medium in sequence. The plate medium includes 0.5% yeast powder, 1% peptone, 50% sodium chloride, and the pH value is 8.2; the shake flask seed medium includes 0.5% yeast powder, 1% peptone, 50% sodium chloride, the pH value is 8.2; the shake flask fermentation medium includes 0.1% yeast powder, 3% glucose, 50% sodium chloride, and the pH value is 9.0.

[0036] The prepar...

Embodiment 2

[0046] This example provides a method for preparing polyhydroxyalkanoate, which is obtained by inoculating Halomonas species into a fermentation medium for fermentation. The halomonas species is obtained by activating the halomonas and culturing seeds. The Halomonas was obtained by applying the lake water of Aiding Lake in Qinghai to the separation and purification medium, and culturing it statically at 33° C. for 24 hours.

[0047] The halomonas species is obtained after seed culture is carried out through plate culture medium activation, shake flask seed culture medium and shake flask fermentation culture medium in sequence. The plate medium includes 0.5% yeast powder, 1% peptone, 50% sodium chloride, and the pH value is 8.2; the shake flask seed medium includes 0.5% yeast powder, 1% peptone, 50% sodium chloride, the pH value is 8.2; the shake flask fermentation medium includes 0.1% yeast powder, 3% glucose, 50% sodium chloride, and the pH value is 9.0.

[0048] The prepar...

Embodiment 3

[0059] This example provides a method for preparing polyhydroxyalkanoate, which is obtained by inoculating Halomonas species into a fermentation medium for fermentation. The halomonas species is obtained by activating the halomonas and culturing seeds. The Halomonas was obtained by applying the lake water of Aiding Lake in Qinghai to the separation and purification medium, and culturing it statically at 33° C. for 24 hours.

[0060] The halomonas species is obtained after seed culture is carried out through plate culture medium activation, shake flask seed culture medium and shake flask fermentation culture medium in sequence. The plate medium includes 0.5% yeast powder, 1% peptone, 50% sodium chloride, and the pH value is 8.2; the shake flask seed medium includes 0.5% yeast powder, 1% peptone, 50% sodium chloride, the pH value is 8.2; the shake flask fermentation medium includes 0.1% yeast powder, 3% glucose, 50% sodium chloride, and the pH value is 9.0.

[0061] The prepar...

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Abstract

The invention belongs to the technical field of preparation of polyhydroxyalkanoate, and discloses a preparation method and application of polyhydroxyalkanoate. The preparation method comprises the following steps: inoculating a fermentation culture medium with a halomonas strain for fermentation culture; after the fermentation culture is performed for 6-10 hours, the mass percent of the carbon source is controlled to be 1.3-1.6%, and the mass percent of the nitrogen source is controlled to be 0.015-0.05%; after the fermentation is performed for 30 to 40 hours, the mass percent of the nitrogen source is controlled to be 0.01 to 0.015 percent; and after the fermentation culture time is 45-50 hours, stopping controlling the concentration of the nitrogen source. By controlling the concentration of the carbon source, the concentration of the nitrogen source, the adding time and other factors, the preparation method of the polyhydroxyalkanoate improves the conversion rate of the polyhydroxyalkanoate, reduces the usage amount of sodium hydroxide, and also has the advantages of being simple and easy to industrialize.

Description

technical field [0001] The invention belongs to the technical field of polyhydroxyalkanoate preparation, in particular to a preparation method and application of polyhydroxyalkanoate. Background technique [0002] Polyhydroxyalkanoate is a kind of polyester synthesized by microorganisms in vivo, which has physical and mechanical properties similar to chemically synthesized plastics, as well as biodegradability, biocompatibility, optical activity, piezoelectricity, etc. that chemically synthesized plastics do not have. Excellent properties such as resistance and gas barrier. Polyhydroxyalkanoate is degradable in nature and causes little environmental pollution. It is a renewable biological resource and is conducive to sustainable development. It has great potential in many fields such as agriculture, packaging materials, drug slow-release carriers, and tissue engineering. application potential. [0003] Halomonas is often used in the fermentation of polyhydroxyalkanoate. H...

Claims

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Application Information

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IPC IPC(8): C12P7/625C12N1/20A61K47/34C08L67/04C12R1/01
Inventor 银会娟沈宏伟何世琪吕金艳张恒文孙磊
Owner MEDPHA CO LTD
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